In vitro development ofXenopus skin glands producing 5-hydroxytryptamine and caerulein

The granular glands of amphibian skin synthesize and store a large amount of bioactive amines and peptides which are structurally similar to mammalian brain-gut peptides. To investigate the development of peptide- and amine-producing cells in the granular glands, pieces of dorsal skin taken at various stages fromXenopus laevis tadpoles were cultured, and the contents of caerulein and 5-hydroxytryptamine (5-HT) were measured. When pieces of skin from tadpoles at stages 57 to 60 (Nieuwkoop and Faber stages) were cultured in a medium containing 10% fetal calf serum (FCS medium) or one containing FCS treated with charcoal (chFCS medium), the caerulein and 5-HT levels were increased for the six days of the incubation period. The caerulein content was lower in the chFCS medium than in the FCS medium. Addition of thyroxine to the chFCS medium had no significant effect on the caerulein content. These results show that the caerulein-and 5-HT-producing cells of the granular glands can develop in a culture system with FCS- or chFCS-containing media, and suggest that FCS contains substances which are absorbed by charcoal and stimulate development of the amine- and peptideproducing cells of the glands. In a preliminary search for correlation between caerulein and 5-HT synthesis, addition of 5-hydroxytryptophan (5-HTP), a precursor to 5-HT, to the FCS medium increased 5-HT content and, conversely, caused significant decrease in caerulein content, suggesting that accumulation of caerulein in the granular glands is influenced by the amount of 5-HT synthesis. These studies indicate that this culture system is a useful model for investigating the development of peptide- and amine-producing cells.     

杂种石斛兰组织培养的研究

本文用引进的泰国石斛兰熊猫１号（ＰＡＮＤＡＮｏ．１）与熊猫２号（ＰＡＮＤＡＮｏ．２）杂交，并将杂交种子经组织培养，获得无病毒试管苗结果表明：在附加６－ＢＡ０．１～１ｍｇ／Ｌ和ＮＡＡ０．５～１．５ｍｇ／Ｌ的改良ＫＣ及改良ＭＳ培养基上，原球茎的发生率达９０％以上，增殖８～１０倍．生根壮苗培养阶段，以附加５％～１０％马铃薯提取液和５％～１０％香蕉汁（不加任何激素）的处理效果最佳．用冷开水代替蒸馏水，食用白糖代替试剂蔗糖，对杂交石斛兰试管苗的生长无明显影响．     

中部地区企业留人之道

以“中部崛起”为背景,阐述了在社会主义市场经济条件下,中部地区企业如何搞好人力资源开发与管理,从而留住人才,促进中部地区经济发展和社会进步。     

构建西部丝绸之路沿线非物质文化保护体系

丝绸之路是古代中国走向世界的窗口,在这条道路上古代中国得以与西方文明交融交汇,共同促进了世界文明的进展。建立一套保护丝绸之路沿线非物质文化遗产的科学办法,并对此进行研究,这不仅对这些地区的非物质文化保护具有积极的促进和示范作用,而且对于我国构建适合自己国情的非物质文化保护体系也有着积极的促进作用。     

Improved Non-Sieving Quadratic Sieve

In this paper, we give about prime numbers and Blum two theorems and one guess integers.We prove the two theorems about Blum integers.Combining the guess with the primitive non-sieving quadratic sieve,we proposed a improved non-sieving quadratic sieve（INQS）.In INQS,we not only reduce the times of squares and modulo n, but also imply another important conclusion,that is,we don＇t need to find the greatest common divisor of two integers as we do in PNQS.By some examples,we compare it with the primitive non-sieving quadratic sieve（PNQS）. It＇s faster to factor a integer by using improved non-sieving quadratic sieve than the primitive one.     

组织培养法制取鸡腿菇母种的实验研究

利用组织培养方法，以野生鸡腿菇作实验材料，筛选出较理想的母种培养基，为鸡腿菇的驯化栽培提供了母种。     

小麦花粉培养中蔗糖浓度外源激素低温前处理对启动细胞分裂的作用

以4个小麦品种为材料，对培养基中的蔗糖浓度、外源激素以及低温前处理等对高体花粉中小孢子细胞分裂启动的诱导做了深入研究，通过不同蔗糖浓度对花粉脱分化的诱导效果比较，发现3％的蔗糖浓度适用于小麦花粉培养，而较高蔗糖浓度（9％）不利于小麦花粉培养，高于9％蔗糖浓度不能启动花偻粒的脱分化及雄核发育，2，4－D在诱导花偻粒转向孢子体发育的过程中起主要作用，对幼穗花药的低温前处理有利于花粉细胞分裂的启动，适宜的胶处理条件是7℃6-12d6-9d7℃前处理于25－27℃下24－36h，更有利花粉细胞分裂的启动。     

山东史前文化发达的地理基础--兼论考古学与历史地理学的结合

山东地区的史前文化十分发达 ,这一发达与全新世中期暖湿的气候直接相关。同时 ,山东地区临海的区位、适中的海拔、多样的地表形态、放射状的水系 ,也为古人类的生存提供了良好的空间。在深入认识人类与其生存环境的关系这一问题上 ,考古学与历史地理学的结合 ,无疑会给彼此带来生机与活力     

透析美国文化与美国人的思维

美国历经二百多年的发展,如今已成为世界上科技领先、经济发达、实力雄厚的国家,并孕育了丰富多彩的文化。笔者通过透析美国文化,向人们展示了美国人所拥有的创新思维品质,并希望能把这一思维品质融入到我们的文化中来,使我们的文明古国更加生机盎然,永远屹立在世界的东方。     

旧厂房再利用的策略与方法——以厦门市文化艺术中心建设为例

厦门产业结构调整后，城区内的工业企业向城市外围搬迁，工业厂房的利用和改建已成为城市决策者需要面对的一个问题．本文以厦工西廓厂区改建工程即厦门市文化艺术中心为例，探索分析了该方案从城市、建筑、室内空间以及建筑技术等方面解决旧工业建筑利用问题的策略与方法，证明了旧建筑再利用的可行性.