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Cone snails (Conus) elaborate a series of conotoxin (CTX) peptides in their venoms to paralyze their prey. Many of these toxins specifically block ion channels in vertebrate and invertebrate neuromuscular systems[1]. The pattern of Cys residues is quite conserved in most CTXs and can be arranged in three major frameworks: CCCC (a-CTX), CCCCCC (m-CTX), CCCCCC (w-CTX). These peptides have been used as important tools in receptor and ion channel studies[2]. Among these toxins, w-CT… 相似文献
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对国内A型肉毒杆菌毒素基因测序,分析了解毒素基因结构并推测其氨基酸序列。提取肉毒杆菌总DNA,利用自行设计的引物对目的基因进行PCR扩增,把目的基因导入E.Coli JM109,筛选含目的基因的阳性克隆菌进行测序和分析,从而了解毒素基因结构并推测其氮基酸序列。对A型肉毒杆菌毒素基因的核苷酸序列成功测序并与GenBank中的相应序列比较,其序列同源性为96%。推测其氨基酸序列同源性为100%。成功地对A型肉毒杆菌毒素的基因进行了测序,这为肉毒杆菌毒素中毒的快速诊断,以及进一步探求以基因工程方法生产此毒素奠定基础。 相似文献
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Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy 总被引:3,自引:0,他引:3
An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 10^7 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection. 相似文献