hsBLyS分泌型表达载体构建及CHO表达

本实验引物延伸合成人红细胞生成素信号肽序列；信号肽序列和人可溶性B淋巴细胞刺激因子(human soluble B Lymphocyte Stimulator，hsBLyS)基因重叠延伸拼接成融合基因；融合基因插入pcDNA3、pcDNA3．1、pEFneo质粒；信号肽引物设计时，突变同义密码，最终重组质粒成为分泌表达质粒；磷酸钙共沉淀将表达质粒和标志质粒pSV-dhfr，共转染CHO-dhfr^-细胞；选择培养液筛选，氨甲喋呤扩增表达，获稳定表达rhsBLyS细胞株；ELISA检测浓缩表达上清，结果表明：rhsBLyS表达量由0．13μg/mL上升至0．55μg/mL。     

A STUDY ON FLORISTIC FEATURE AND DIVERSITY OF DEHANG REGION SEED PLANTS IN HUNAN

1IntroductionDehangisascenicspotlocatedatthesouthofWulingMTsintheCentralChil1a,between2815'and2843'N,lo93o'andlO945lE,risingfroml89.4mto964.6mabovesealevel,withanareaof225km.Thisre-gionhasacomplicatedtopography.Itissituatedinsubtropicmonsoonclimateareaandrichinlimestone.Ev-ergreebroad-leavedforest,Bambooforestandlowsimountainevergreenconiferousforestaremainvegeta-tionshere'Moreover,Miaonationalitypeoplehhvebeenlivinghereforalongtimeanditenjoysgreatnation-a1navour.Thenoraisrichandthevegeta…     

湖南植物新记录18种

报道了湖南植物分布新记录属6个:野丁香属(Leptodremis Wall.)、双参属(Triplostegia Wall.ex DC.)、幌菊属(Ellisiophyllum Maxim.)、呆白菜属(Triaenophora Soler.)、石蝴蝶属(Petrocosmea Oliv.)、克拉莎属(Cladium P.Browne);新记录种18个:薄皮木(Leptodermis oblonga Bunge)、双参(Triplostegia glandulifera wall.ex Dc.)、地海椒(Physaliastrum sinensis(Hemsl.)Kuang)、幌菊(Ellisiophyllum pinnatum(Wall.)Makino)、呆白菜(Triaenophora rupestri(Hemsl.)Solereder.)、中华石蝴蝶(Petrocosmea sinensis Oliv.)、华克拉莎(Cladium chinense Ness.),小舌紫菀(Aster albescens(DC.)Hand-Mazz.),钻形紫菀(Aster subulatus Michx.),大狼杷草(Bidens frondose L.),粗齿天名精(Carpesium trachelifolium Less.),马刺蓟(Cirsium monocephalum(Van.)Levl.),窄头橐吾(Lugularia stenocephala(Maxim.)Matsum.et Koidz.),巴东风毛菊(Saussurea henryi Hemsl.),利马川风毛菊(Saussurea leclerei Levl.),滇黔蒲儿根(Sinocenecio bodiuieri(Vant.)B.Nord.),南川斑鸠菊(Vernonia bockiana Diels),神龙黄( )菜(Youngia henryi(Diels)Babc.et Stebb.).     

Phytotoxins and their involvement in plant diseases. Introduction

Bacterial and fungal plant pathogens are known to produce diffusible toxins in infected plants. These phytotoxins are harmful to plants in very low concentrations, and many reproduce at least some of the symptoms of the relevant bacterial or fungal disease. Several phytotoxins have been shown to be involved in pathogenesis. Recent years have seen substantial progress in our knowledge about the nature, structure and mode of action of phytotoxins, and this is briefly reviewed. Finally, possible applications in fields other than plant pathology are mentioned.The Editors wish to thank Prof. A. Ballio and Prof. A. Graniti for coordinating this multi-author review.     

4-Hydroxynonenal-modified amyloid-beta peptide inhibits the proteasome: possible importance in Alzheimer's disease

The amyloid β-peptide (Aβ) is a 4-kDa species derived from the amyloid precursor protein, which accumulates in the brains of patients with Alzheimer’s disease. Although we lack full understanding of the etiology and pathogenesis of selective neuron death, considerable data do imply roles for both the toxic Aβ and increased oxidative stress. Another significant observation is the accumulation of abnormal, ubiquitin-conjugated proteins in affected neurons, suggesting dysfunction of the proteasome proteolytic system in these cells. Recent reports have indicated that Aβ can bind and inhibit the proteasome, the major cytoslic protease for degrading damaged and ubiquitin-conjugated proteins. Earlier results from our laboratory showed that moderately oxidized proteins are preferentially recognized and degraded by the proteasome; however, severely oxidized proteins cannot be easily degraded and, instead, inhibit the proteasome. We hypothesized that oxidatively modified Aβ might have a stronger (or weaker) inhibitory effect on the proteasome than does native Aβ. We therefore also investigated the proteasome inhibitory action of Aβ _1–40 (a peptide comprising the first 40 residues of Aβ) modified by the intracellular oxidant hydrogen peroxide, and by the lipid peroxidation product 4-hydroxynonenal (HNE). H₂O₂ modification of Aβ _1–40 generates a progressively poorer inhibitor of the purified human 20S proteasome. In contrast, HNE modification of Aβ _1–40 generates a progressively more selective and efficient inhibitor of the degradation of fluorogenic peptides and oxidized protein substrates by human 20S proteasome. This interaction may contribute to certain pathological manifestations of Alzheimer’s disease Received 26 September 2000; accepted 26 September 2000     

湖南通道侗族自治县种子植物属的区系研究

通道侗族自治县种子植物区系715属，归为14种区系成分，其中，泛热带分布（132属，占20％）、北温带分布（106属，占16．06％）、热带亚洲分布（100属，占15．15％）及东亚分布（87属，占13．18％）所占比例较大；热带地理成分349属，占总属数的52．88％，温带地理成分311属，占总属数的47．12％，与相邻植物区系的比较表明，通道植物区系与花坪和都庞岭的关系较为密切，而与三清山及八大公山的相似性程度稍低，在与之相比较的7个相邻植物区系中，除广西的花坪外，通道的R／T值最高，表明通道植物区系的热带性程度高于相近纬度的其他6个地区，该区植物区系总体上表现区系成分复杂多样，显示出明显的过渡性质，而在该县南部（传素—独坡一线以南）汇集有丰富的华南热带成分，显示出热带北缘性质。     

植物基因克隆的方法和策略

芬子生物学的迅速发展对克隆植物基因起了积极的推作用，本篇旨在介绍植物基因克的策略及研究进展。     

棉蝗的寄主及生活习性观察

报道了棉蝗（Ｃｈｏｎｄｒａｃｒｉｓｒｏｓｅａｒｏｓｅａ（ＤｅＧｅｅｒ））取食的３５科６８种植物。对该蝗的生活习性也作了简要描述。     

齐齐哈尔市园林绿化树种的选择和配置

本文运用大量的实例，从齐齐哈尔市园林绿化树种选择和应用的角度出发，分析了园林绿化中存在的问题，提出适地适树，在综合条件允许的情况下，扩大选择范围，改变传统配置模式，创造出更加优美的城市环境。