发酵花生秧粉对育肥猪生产性能的影响及其促生长机理

研究经复合菌剂发酵后的花生秧粉对育肥猪生产性能的影响及其促生长机理,探讨利用发酵花生秧粉进行育肥猪节粮健康养殖的可行性.以大白×长白×杜洛克三元杂交猪为供试猪,共分为3组,对照组饲喂常规饲粮,实验组Ⅰ饲喂90％常规饲粮+10％发酵花生秧粉,实验组Ⅱ饲喂80％常规饲粮+20％发酵花生秧粉,实验为期40 d,测定并计算各组供试猪的料重比与增重成本,同时采集各组供试猪的新鲜粪样,测定粪样中菌群结构,常规消化酶活力以及常规营养物质含量的变化情况.结果显示:实验组工、Ⅱ与对照组相比,其料重比分别降低11.70％和4.96％,增重成本分别下降9.45％和0.48％,全程成本分别降低4.71％和9.23％;实验组Ⅰ、Ⅱ菌群数量与对照组相比,其大肠杆菌数量分别降低48.75％ (P＜0.01)和40.99％ (P＜0.01),双歧杆菌数量分别提高1.89％ (P＞0.05)和22.78％ (P＜0.05),乳酸菌数量分别提高8.17％ (P＞0.05)和17.13％(P＜0.05),芽孢菌株数量分别提高18.14％ (P＜0.05)和5.42％(P＞0.05);实验组Ⅰ、Ⅱ消化酶活力与对照组相比,纤维素酶活力分别提高15.68％ (P＜0.01)和4.10％ (P＞0.05),蛋白酶活力分别提高2.41％ (P＞0.05)和6.28％ (P＞0.05),淀粉酶活力分别提高17.07％ (P＜0.01)和14.02％ (P＜0.05),脂肪酶活力分别提高2.58％ (P＞0.05)和4.78％(P＞0.05);实验组Ⅰ、Ⅱ试猪粪便中常规营养物质含量与对照组相比,粗蛋白含量都降低9.09％ (P＜0.01),粗纤维含量分别降低5.71％(P＞0.05)和2.86％(P＞0.05),木质素含量都降低4.00％ (P＞0.05),有机酸含量分别降低7.56％(P＜0.01)和9.33％ (P＜0.01),粗脂肪含量分别降低6.67％ (P＞0.05)和14.67％ (P＜0.01),而灰分含量变化不大.研究结果表明:利用发酵花生秧粉饲喂育肥猪可促进动物生长,且大大降低生产成本,为发酵花生秧粉在育肥猪的饲喂养殖中奠定了理论基础.     

浅析智猪博弈与网络产品价格策略

分析了网络企业提供免费服务的原因,并结合智猪博弈提出网络产品价格策略的建议。     

开展农村养殖技术承包促进社会主义新农村建设——优种瘦肉型商品猪增产技术推广

阐述了农村养殖技术承包的目的、内容、方式，提出了承包的技术措施，分析了承包后带来的经济效益。     

痛苦的哲学家和快乐的猪——浅谈电视剧《金婚》中两代人的爱情观

郑晓龙导演的电视剧《金婚》是一部以爱情和婚姻为主题的片子。该剧通过对两代人爱情婚姻生活的演绎,展示了两代人不同的爱情观——上一辈人对爱情认真而执着,而下一辈人则快乐并且游戏着。面对着爱情婚姻各种各样的病痛,郑晓龙通过《金婚》告诉我们,婚姻的美满需要双方的付出和精心的呵护,只有这样,婚姻才有可能进入"金婚"期。     

DNA fragmentation in leukocytes following repeated low dose sarin exposure in guinea pigs

The objective of this study was to determine levels of DNA fragmentation in blood leukocytes and parietal cortex from guinea pigs following repeated lowlevel exposure to the chemical warfare nerve agent (CWNA) sarin. Guinea pigs were injected (s.c.) once a day for 10 days with saline, or 0.1, 0.2, or 0.4 LD₅₀ (50% mean lethal dose) sarin dissolved in sterile physiological saline. Blood and parietal cortex was collected after injection at 0, 3, and 17 days recovery and evaluated for DNA fragmentation using single-cell gel electrophoresis (Comet assay). Cells were imaged using comet analysis software and three parameters of DNA fragmentation measured: tail length, percent DNA in the tail, and tail moment arm. Repeated low-dose exposure to sarin produced a dose-dependent response in leukocytes at 0 and 3 days post-exposure. There was a significant increase in all measures of DNA fragmentation at 0.2 and 0.4 LD₅₀, but not at 0.1 LD₅₀. There was no significant increase in DNA fragmentation in any of the groups at 17 days post-exposure. Sarin did not produce a systematic dose-dependent response in parietal cortex at any of the time points. However, significant increases in DNA fragmentation at 0.1 and 0.4 LD₅₀ were observed at 0 and 3 days post-exposure. All measures of DNA fragmentation in both leukocytes and neurons returned to control levels by 17 days post-exposure, indicating a small and non-persistent increase in DNA fragmentation following repeated low-level exposure to sarin. Received 23 July 2007; received after revision 23 August 2007; accepted 3 September 2007 Research was conducted in compliance with the Animal Welfare Act, and other Federal statutes and regulation relating to animals and experiments involving animals and adheres to the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH publication 85-23. The views of the authors do not purport to reflect the position of the Department of the Army or the Department of Defense (para 4-3), AR 360–365.     

青藤祛湿外敷散对豚鼠的长期毒性研究

考察青藤祛湿外敷散反复外敷给药对豚鼠的长期毒性,以评价其长期用药的安全性.设计一个对照组和高、中、低三个给药组,豚鼠每日外敷给药1次,连续13周.试验期和恢复期分别对豚鼠的外观行为、体重、血液学及血液生化学、病理组织学等指标进行检测.与对照组相比,各给药组的检测指标统计学分析均无显著性差异.青藤祛湿外敷散经13周反复外敷给药,未发现毒性靶器官.可确定青藤祛湿外敷散最大毒性剂量为20.250 g/(kg·d).     

低聚木糖对仔猪和生长肥育猪生产性能的影响研究

研究表明,添加低聚木糖可使仔猪日增重平均提高0.69%,采食量平均减少20.53%,饲料效率平均提高20.32%,仔猪腹泻率平均降低20.51%.在生长肥育猪日粮中添加微生态制剂、低聚木糖、微生态制剂与低聚木糖合用分别使生长肥育猪日增重6.5%,7.5%,14.0%;日采食量提高4.8%,5.3%,10%;料肉比分别下降1.6%,2.2%,3.6%;提高经济效益0.82%,0.6%,1.35%.     

外源hDAF基因对转基因猪影响的初步研究

应用显微注射方法将外源hDNA基因导入湖北白猪的受精卵,获得了相应的转基因猪,4．2,8．0kb两种导入外源基因片断移植后死胎率分别为20．00％,39．71％,差异不显著,8．0kb基因产仔畸形率为11．76％,而4．3kb基因无畸形,结果表明,原核基因对转基因猪有明显的致畸作用,G0代转基因猪60日龄重,6月龄重对照组差异均不显著,表明外源基因的整合位点较理想。     

民族地区可持续发展的博弈分析

我中国绝大多数民族地区经济发展落后,人口、资源和环境等与经济社会发展不相协调,面临着生态破坏、环境污染等问题.因此,民族地区可持续发展变成了是一个囚徒困境博弈过程或智猪博弈过程,甚至有时出现变成了逆向选择的后果.为避免这种博弈就成为民族地区小康社会建设重大而紧迫的问题.     

豚鼠气管平滑肌细胞培养

目的 建立一种简便、快捷的豚鼠气管平滑肌细胞培养方法。方法 幼年豚鼠气管平滑肌组织块原代培养 法。结果 豚鼠气管平滑肌组织块贴壁5～8d后可见细胞自组织块周围长出,细胞长梭形,核位于中央,呈辐射状 向四周生长。两周后细胞长满瓶壁。经α-平滑肌肌动蛋白单克隆抗体(α-smooth muscle actin monoclonal antibody, α-SMA)免疫组化染色鉴定,获得的细胞为平滑肌细胞。结论 豚鼠气管平滑肌组织块培养法可快速获得数量充足 的气管平滑肌细胞。