小儿断指再植39例治疗体会

目的：总结小儿断指再植经验。方法：从1990年7月-2006年4月进行小儿断指再植39例42指，对各类型的小儿断指再植进行探讨。结果：小儿断指再植总成活率90．48％。结论：严格断指再植适应证；彻底的清创；精确无误的吻合血管；认真细致地修复各种组织；果断的处理血管危象；早期功能锻炼等，是提高断指再植成活率及功能康复的重要因素。     

Cholesterol content and cholesterol esterifying activity of various organs in guinea pigs

Summary In various organs of the guinea pig, the total cholesterol content of an organ was significantly correlated with the percentage of esterified cholesterol present in this organ. Cholesterol esterifying capacity was shown in most organs, with highest activities in the adrenals, the spleen and the liver. The significant correlation found between the cholesteryl ester content of an organ and its acyl cholesterol acyltransferase activity suggests a possible role of this enzyme in determining the level of the total and esterified cholesterol in a tissue.This work was supported by a grant Crédit aux Chercheurs du Fonds National de la Recherche Scientifique of Belgium.Acknowledgments. I am indebted to Prof. C. Harvengt for his stimulating interest and helpful suggestions. I wish to thank Y. van Nieuwenhuyze and J. Costermans for their valuable laboratory assistance and Dr H. Baudon for pathological studies.     

应用多元回归方程和列线图估测淮南猪瘦肉量的研究

本文根据淮南猪原种场纯繁群1987——1989年的胴体测定资料,在对屠宰后几个与胴体瘦肉量有关的易测性状进行相关分析和通径分析的基础上,建立了估测淮南猪瘦肉量的“最优” 回归方程:(?)=1.9162 0.0717x_l 0.3973x_2 0.0937x_3(F=17.594,(?)=0.0916公斤),同时,制成了一种简化的列线图,应用该多元回归方程和列线图估测瘦肉量,不仅可节省组织剥离花费大量的人力和物力,而且准确性好,具有较广泛的适用范围和较大的实用价值。     

Minoxidil-induced cardiac hypertrophy in guinea pigs

To investigate whether during cardiac hypertrophy changes occur in contractile protein composition and in mechanical and energetic properties of the myocardium, contractile protein composition, isometric force and adenosine triphosphate (ATP) consumption were studied in control and hypertrophied guinea-pig hearts. Cardiac hypertrophy was induced by adding minoxidil (120 or 200 mg/l) to the drinking water. Protein analysis was performed by one-dimensional gel electrophoresis. The myosin heavy-chain (MHC) composition was determined in an enzyme-linked immunosorbent assay (ELISA). ATP consumption and force development were simultaneously measured during isometric contraction in chemically skinned trabeculae. Histochemical analysis of cross-sectional area of cardiomyocytes and interstitial space was performed on the left ventricular tissue of 200 mg/l minoxidil-treated and control guinea pigs. Minoxidil treatment (120 and 200 mg/l) significantly increased left ventricular dry weight normalized for body weight by 19 ± 4 and 24 ± 4%, respectively. No significant differences were found in the cellular cross-sectional area, while interstitial space was slightly decreased in minoxidil-treated hearts. In left ventricular trabeculae of 200 mg/l minoxidil-treated guinea pigs, ATPase activity was slightly less than in those of control guinea pigs, whereas force did not differ significantly. Calcium sensitivity of force and ATPase activity were not affected by minoxidil treatment. Gel electrophoresis revealed no difference in contractile protein composition, but a tendency towards a lower amount of α-MHC in the minoxidil-treated hearts was found in ELISA. Received 1 February 1999; accepted 15 March 1999     

在役压力容器缺陷评定研究进展

回顾和总结了在役压力容器缺陷评定和容限分析的发展现状 ,并主要对失效评定图、概率方法、智能方法 (尤其人工神经网络方法 )和疲劳工程评定方法新进展进行了综述 .提出了今后的发展方向和需要着重开展的工作 .     

猪肝组织肝小叶平均间距的一种估计方法

利用AR倒谱法对猪肝组织的回波信号进行了分析,对猪肝组织中肝小叶的平均间距进行了估计,结果表明：AR倒谱能有效的反映猪肝组织的微观结构特征,说明AR倒谱是软组织中散射信号分析与软组织散射子平均间距估计的一种有效方法。     

贵州小型猪心肌细胞超微结构的观察

观察贵州小型猪心肌细胞的形态和结构。方法应用透射电镜进行观察。结果贵州小型猪心肌细胞的肌丝有规律排列形成的周期性横纹明显可见,可分辨出Z线I、带、A带、H带和M线;在肌丝周围有丰富的线粒体;肌浆网不丰富,横小管十分细小,未见二联体;在Z线处,可见心肌细胞之间含有3种连接方式的特有连接结构——润盘。结论贵州小型猪心肌细胞的超微结构与人类极为相似,但也有其自身的特点。     

藏猪血液蛋白多态性的研究

采用聚丙烯酰胺凝胶垂直平板电泳法研究了四川、云南两地藏猪的Ｔｆ、Ｐａ、Ｐｏ、Ａｍ Ⅰ、Ａｍ Ⅱ、Ｃｐ、Ｈｐ、Ｆｓ基因座的血液蛋白多态性,结果表明：（１）所测基因座均呈现多态性;（２）两地藏猪间存在由于不同地理环境、选育水平等所导致的地方类群差异,但基因频率差异不显著．     

小型猪2 型糖尿病模型研究进展

摘要: 小型猪在生理学、解剖组织学、饮食结构和营养代谢等方面与人高度相似,已成为人类疾病模型研究的关注热点。本文在简要介绍2 型糖尿病发生机制和啮齿类动物模型的局限后,重点阐述了高脂高能量饮食和药物诱导小型猪2 型糖尿病模型的特点,及基因工程小型猪模型的研究进展和存在不足,并对小型猪2 型糖尿病模型的发展进行了展望。     

局部非冻结性冷暴露对豚鼠周围神经形态结构的影响

在３６ 只成年雄性豚鼠上观察了动物后半身受到４ ±２ ℃局部冷暴露后周围神经形态结构的变化。结果表明：冷暴露后坐骨神经内的微血管充血;有髓纤维从施兰氏切迹和郎飞氏结附近开始呈现进行性的髓鞘变性,以暴露后３ 天最为严重。此时电镜显示髓鞘板层分离、断裂,间距明显增大。冷暴露后７ 天显示纤维再生与变性并存。与细纤维相比,粗纤维较早发生变性,也更为明显。在非冷暴露部位的上肢桡神经上未观察到上述变化