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141.
General pedigrees are very common in farm animals,and the recent availability of large panels of SNPs in domestic species has given new momentum to the search for the mutations underlying variation in quantitative traits.In this paper,we proposed a new transmission disequilibrium test approach,called the pedigree transmission disequilibrium test,which deals with general pedigrees and quantitative traits in farm animals.Compared with the existing pedigree disequilibrium test (PDT) and general linear model-based method QTDT,our approach performed better with higher power and lower type I error,especially in scenarios where the quantitative trait locus (QTL) effect was small.We also investigated the application of our approach in selective genotyping design.Our simulation studies indicated that it was plausible to implement a selective genotyping strategy in the proposed pedigree transmission disequilibrium test.We found that our approach performed equally well or better when only some proportion of the individuals in the two tails were genotyped compared with its performance when all the individuals in the pedigree were genotyped. 相似文献
142.
Lifei Liu Jingjing Lei Haiyun Liu Qinjie Zou Yuhua Sun Yancheng Xu Huimin Bi Fengjiao Deng Xueling Shao Siyang Liu 《武汉大学学报:自然科学英文版》2010,15(2):171-175
In this study, we selected 10 susceptible SNPs loci to investigate their contribution to susceptibility to type 2 diabetes in Han Chinese among Hubei population. We genotyped SNPs rs5219, rs1801282, rs1470579, rs1111875, rs1081661, rs7754840, rs4506565, rs13266634, rs4402960, and rs5643981 by using the method of polymerase chain reaction-ligase detection reaction (PCR-LDR). In a case-control study, we have genotyped the 10 candidate susceptibility SNP loci, and here, we reported that the SNP rs5219 in KCNJ1... 相似文献
143.
SHAHID Muhammad Qasim 《科学通报(英文版)》2010,55(13):1255-1262
Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S5n allel... 相似文献
144.
Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions.To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3.Unlike Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on severity.Additive effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection type.Single marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN. 相似文献
145.
WADA将基因或细胞兴奋剂定义为“能增强运动能力的基因,遗传元素和/或细胞的非治疗性的使用”.遗传学和基因学的新的研究结果不仅将用于疾病的诊断和治疗,而且还将用于试图增强机体的运动能力.用于治疗疾病的基因疗法,如贫血(EPO基因)、肌肉营养不良(IGF—I基因)和外周血管疾病(血管内皮生长因子基因)等,都可能被用于兴奋剂.为保护运动员的健康和保证公平竞争,IOC、WADA和ISF(国际体育联合会)已将提高运动能力的药物和方法定义为基因兴奋剂,并禁止其使用. 相似文献
146.
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148.
生物工程的现状与展望 总被引:1,自引:0,他引:1
叶峻 《重庆大学学报(自然科学版)》1990,13(6):31-36
阐述了生物工程在农牧、食品、医药工业、生物采矿、生物化工、环境保护、生物能源、生物医学工程等主要领域的发展现状;分析讨论了美国、日本、西欧等国家生物工程的竞争态势;展望了生物工程的未来前景。 相似文献
149.
王俐琳 《杭州师范学院学报(社会科学版)》1994,(6)
真核生物受热击及其他应激作用后能诱导产生HSP。HSP在生物体内起着重要的生理功能。研究热击反应有助于搞清植物基因表达调控的机理,这也是科学家们近几年来研究的热点。随着植物基因工程技术的完善和发展,这一研究进展很快。HSE、HSF和HSP在热击基因转录和调控过程中起着非常重要的作用。 相似文献
150.
随着无血清培养细胞时间的延长,红胞合成Rb蛋白量和磷酸化水平逐渐降低,当细胞生长停止时,Rb蛋白处于低磷外化状态.正丁酸钠可诱导HL60终端分化为单核-巨噬细胞;视黄酸、二甲基亚砜使HL60分化为粒细胞.尽管分化途径不同.但是终端分化的细胞都只能合成低磷酸化的Rb蛋白.结果表明当细胞处于生长停滞状态,细胞都只能合成低磷酸化的Rb蛋白. 相似文献