NAP1基因和ARHP基因启动子区的生物信息学分析

采用进化足迹法,利用生物信息学在线软件,分析了鼻咽癌相关肽基因NAP1和成人视网膜假定蛋白基因ARHP的启动子结构,在人和小鼠NAP1基因的启动子保守区内预测出38个共同的转录因子结合部位,在人和小鼠ARHP基因的启动子保守区内预测出44个共同的转录因子结合部位,分析结果为NAP1基因和ARHP基因调控区的分析与鉴定,基因表达调控机制的研究提供了重要的参考.     

甜菜夜蛾核多角体病毒基因组13.7～21.6 m.u.区域的分析

甜菜夜蛾核多角体病毒(Spodoptera exigua multicapsid nucleopolyhedrovirus,SeMNPV)基因组13．7～21．6m．u．区域是SeMNPV的重组热点区。以SeMNPV美国分离株SeUS1为模板,长片段PCR对SeUS1的13．7～21．6m．u．区域扩增,得到11．3kb,2．0kb和0．7kb3个片段。对照已发表的SeMNPV基因组序列,11．3kb片段是具完整SeMNPV序列基因型的产物,2.0kb和0．7kb片段表明SeUS1中还存在两种缺失突变株。对2．0kb和0．7kb片段的序列分析揭示了这两株突变株在缺失部位的DNA一级结构。对三株重组SeMNPV病毒(SeXDl、SeXD2和SeXD3)的分析发现,它们和野生型病毒SeUS1中的一个基因型一样,均缺失了SeMNPV nt 18513～29105之间长10593bp的片段,暗示SeUS1中一株自发形成的缺失突变体在离体细胞中具有复制优势。将该片段基因排列与其它10种基因组序列已测定的杆状病毒比较,发现两个在GroupⅡ NPVs中保守的基因簇。最大简约性法对部分基因的系统发育分析表明,缺失片段中某些基因可能存在于杆状病毒分化之前的病毒基因组中。     

Mitochondrial DNA analysis of Bronze Age horses recovered from Chifeng region, Inner Mongolia, China

In this study, mitochondrial DNA (mtDNA) analysis was carried out on 9 Bronze Age horses recovered from Dashanqian and Jinggouzi archaeological sites in Chifeng region, Inner Mongolia. China to explore the origin of Chinese domestic horses. Both mtDNA 16S rRNA gene and control region (D-loop) fragments of ancient horses were amplified and sequenced. The analysis of the highly conservative 16S rRNA gene sequences indicated that the burial environment of Chifeng region is suitable for the preservation of ancient DNA (aDNA). Combing 465 mtDNA D-loop sequences representing different breeds from East Asia, Central Asia, Near East and Europe, we constructed a phylogenetic network to investigate the relationship between ancient and modern horses. The phylogenetic network showed that the 9 horses were distributed into different modern horse clusters which were closely related to them representing a certain geographical distribution. Our results showed that the maternal genetic line of the ancient horses in Chifeng region was highly diversified, which contributed to the gene pool of modern domestic horses and suggested a complex origin of domestic horses in China.