诸葛菜对水分胁迫的生理生化反应和调节适应能力

研究了水分胁迫对诸葛菜叶片的水分状况、渗透调节能力以及活性氧化谢的影响,结果表明：在水分胁迫下,诸葛菜叶片相对含水量,自由水含量下降,束缚水含量上升;可溶性糖,脯氨酸,Ｋ＾＋、Ｃａ＾２＋含量均出现明显的增加,具有一定的渗透调节能力;随着水分胁迫的加剧,Ｏ＾－２产生速率增加,丙二醛积累,导致膜损伤,质膜透性上升,超氧化物歧化酶、过氧化物酶活性均出现“先上升,后下降”的趋势,抗坏血含量则降低,表现出诸     

Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice

The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (PI). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.     

饲料脂肪水平对吉富罗非鱼幼鱼摄食后血脂,血糖的影响

为了研究饲料脂肪水平对吉富罗非鱼(Oreochromis niloticus)血液脂肪代谢的影响,选择均重为(2.63±0.16)g的健康的315尾吉富罗非鱼,随机分成3组,每组3个重复,投喂3组不同脂肪水平的等氮低脂肪组、中脂肪组、高脂肪组饲料(饲料中脂肪含量分别为3.71%、7.67%和16.55%),饲养90 d。禁食48 h再投喂,摄食不同脂肪饲料后0、6、12、24、48 h取样测定试验鱼血清中甘油三酯、胆固醇及血糖含量。结果显示:在48 h内,吉富罗非鱼血液中甘油三酯、胆固醇、血糖均呈现先上升后下降的趋势,在摄食后同一时间点,摄食高脂肪试验鱼血液甘油三酯和胆固醇显著高于低脂肪组(P<0.05),而血糖无显著变化,但有下降的趋势。结果说明:摄食后48 h内,吉富罗非鱼摄食低、中脂肪水平饲料后血液甘油三酯、胆固醇、血糖呈现单峰波变化规律,高脂肪诱导血液甘油三酯合成代谢,但没有改变其变化基本规律。     

不同生长发育期土壤绿藻——集球藻细胞形态结构研究

集球藻是一种广泛分布于生物结皮中的土壤绿藻,能够积累红色素(如虾青素)和油滴,显示出其独特的生理特性和潜在的应用价值.目前对集球藻的细胞结构以及色素积累研究非常匮乏.本文报道应用光学显微镜和透射电境观察集球藻在温室液体培养条件下,细胞形态与超微结构变化.结果表明,培养早期时营养细胞转变为生殖细胞,并通过细胞分裂产生子代...     

江蓠藻膳食纤维对衰老小鼠脂质过氧化的影响

研究江蓠藻膳食纤维的抗脂质过氧化作用.将小鼠随机分为空白对照组、模型对照组、燕麦膳食纤维组,以及江蓠藻膳食纤维高、中、低剂量组.在给药的同时,除空白对照组外,其余各组小鼠每天颈背皮下注射10%的D半乳糖0.25mL,连续给药40 d后,测定小鼠脾指数、胸腺指数,以及血清、心、肝、肾、脑中过氧化氢酶(CAT)、超氧化物岐...     

Identification of high-lipid producers for biodiesel production from forty-three green algal isolates in China

To identify some desirable algal strains for our future research and/or the production of algae-based biofuel, 43 green algal strains were successfully isolated from Chinese freshwaters, and then incubated in the laboratory bioreactors for the growth an doil accumulation investigations. During a 15 d incubation experiment, the accumulations of their biomass and total lipids, together with the lipid productivities for these green algal strains were systematically investigated and compared. Results indicated that the accumulations of biomass for the 43 algal strains ranged from 0.53 g/L to 6.07 g/L during the experiments, with the highest bio mass of 6.07 g/L for green algae Scenedesmus bijuga. The lipid content for the tested algal strains varied from 20% to 51% of the dry biomass at the end of cultiva tion experiments. Green algae Chlorella pyrenoidosa was one of the best oil producers based on our investigations, with the total lipid content of 51% of dry biom ass. Taking the growth rates and the accumulations of intracellu lar lipids into the consideration, 10 strains were considered to have significant potential for biofuel applications. In addition, the lipid productivities of the selected strains were further investigated.     

黄芩苷固体脂质纳米粒的制备及大鼠在体肠吸收研究

选用硬脂酸、单硬脂酸甘油酯混合脂质作为载体,以卵磷脂作为乳化剂制备黄芩苷固体脂质纳米粒(BC-SLN),并考察其大鼠在体肠吸收特性.采用热匀质法(hot homogenization technique,HHT)制备BC-SLN,采用循环灌注技术评价黄芩苷固体脂质纳米粒和黄芩苷溶液大鼠在体肠吸收特性.制得的BC-SLN平均粒径为(150.8±47.2)nm,zeta电位为(-39.85±0.86) mV,载药量为(4.86±0.37)%,包封率为(88.52±0.32)%,大鼠在体肠吸收实验表明,BC-SLN与黄芩苷溶液相比,吸收速率常数Ka和每小时吸收百分率p均呈增加趋势,有显著性差异(P＜0.05),黄芩苷固体脂质纳米粒能够促进黄芩苷大鼠肠吸收.     

内蒙古民族大学152名教师血脂检测结果分析

目的:了解教师血脂水平,通过健康教育改变不良生活方式,预防心脑血管疾病.方法:对152例教职工进行血脂检测;胆固醇和甘油三酯分别采用酶法测定.结果:高血脂检出率较高,60岁以前血TC(总胆固醇)和TG(甘油三酯)异常检出率随着年龄的增高而增高,且50岁前男性异常检出率高于女性,50岁以上的女性TC和TG异常率高于同年龄组的男性.结论:预防和控制高血脂,对不同性别进行针对性的健康指导和有效干预,合理饮食、加强体育锻炼,减少心血管疾病的发生.     

全麻剂作用下Ca~(2 )诱导膜融合的研究

全麻剂通过干扰膜的结构与功能而起作用，研究了四种全麻剂（氯仿、乙醚、乙醇及辛醇）对３ｍｍｏｌＣａ２＋诱导融合影响用ＰＳ：ＤＭＰＣ：Ｃｈｏｌｅｓｔｅｒｏｌ＝５：３：２的人工膜脂质体进行实验，脂质体融合用荧光共振能量转移监测，初步实验表明：在临床浓度范围内，氯仿，乙酸及辛醇能轻微地抑制膜融合，而乙醇在临床浓度附近能促进膜融合，并且随着乙醇浓度的提高其促融合效应变得十分明显，这可能是乙醇分子的极性及通过氢键与磷脂头缔结，并取代部份结构水，减弱水化排斥力而促使膜融合。