Effects of medium nutrition on cell growth and isocamptothecin A and B production by suspension cell culture of Camptotheca acuminata

The effects of initial sucrose concentration, nitrate to ammonium ratio, total N concentration and phosphate concentration in medium on cell growth and isocamptothecin A and B synthesis by suspension call culture of Camptotheca acuminata were investigated in 250 mL shake flasks. 30 g L^-1 sucrose concentration was beneficial to secondary metabolites synthesis. The cell growth and metabolites synthesis were also affected by the ratio of NO3^-/NH4^＋ , and nitrate was tavourable for cell growth. The maximum dry weight was achieved when nitrate was used as the sole N souree. The effect of total initial N on the cell cultures was also investigated with NO3^-/NH4^＋ ratio of 1 ： 2. The final dry cell weight was similar throughout culture period and 50 mM initial N was favourable for secondary metabolite synthesis. 50 mM initial phosphate concentration facilitated both cell growth and secondary metabolites synthesis.     

接种密度对Vero细胞在微载体表面生长的影响

研究了接种密度对Ｖｅｒｏ细胞在微载体表面生长行为的影响,发现培养过程中所获得的细胞最高密度随接种密度的增加而有所提高,当细胞的表面接种密度高于１．０×１０＾５ｃｅｌｌｓ／ｍｇＭＣ时,因贴壁后细胞扩展受到限制而不利于进入对数生长期。就整个Ｖｅｒｏ细胞培养过程,适宜的表面接种密度为３×１０＾－４－７×１０＾４ｃｅｌｌｓ／ｍｇＭＣ,此时可以获得较高的比生长速率和细胞增殖倍数。     

传染性法氏囊病病毒在鸡胚细胞上的优化繁殖

研究了传染性法氏囊病病毒在鸡胚细胞上繁殖上的优化条件，结果表明，鸡胚细胞体外增殖培养后对ＩＢＤＶ的敏感性并不减弱，而且有提高。维持培养基中血清浓度的变化对病毒的繁殖影响不大。     

Scientists and their cultural heritage: Knowledge,politics and ambivalent relationships

For many years, scientific heritage has received attention from multiple actors from different spheres of activity—archives, museums, scientific institutions. Beyond the heterogeneity revealed when examining the place of scientific heritage in different places, an authentic patrimonial configuration emerges and takes the form of a nebula of claims and of accomplishments that result, in some cases, in institutional and political recognition at the national level, in various country all around the world. At the international level, the creation of the international committee dedicated to University Museums and Collections (UMAC) within the International Council of Museums (ICOM) certainly testified from this raising interest in academic heritage and the existence of a specific community concern with it.This article presents numerous initiatives for the preservation of scientific heritage in France, with the goal of analysing the relationship scientists have with their heritage. We argue that scientific communities have a special relationship with heritage, which is characterized by a number of ambiguities. We show that such ambivalences allow analysis of identity, discipline, professional, and social issues operative in defining heritage and being redefined by heritage. To explore these dimensions, we have chosen to present three different case studies. The first traces the institutional uses of heritage by a scientific institution, the Commissariat à l’énergie atomique (CEA), through the transformation of the first French atomic reactor (ZOE) into a museum. The second example describes the initiatives of French astronomers from the mid-1990s to construct a national programme for the protection of astronomy heritage. Lastly, we recount the case of universities, with the example of the Université de Strasbourg.     

英汉动物习语的文化内涵对比

动物词汇作为构成习语的一个重要成分,具有独特的作用和丰富的表现力,是语言词汇不可分割的一个重要组成部分。然而,由于各民族不同的地理环境、文化传统、社会习俗和思维方式,动物被赋予了或同或异的联想意义,因而其动物习语具有独特的民族文化色彩。现运用对比研究法,从动物的联想意义、英汉习语中动物形象的相似和差异,英汉含动物词汇的习语所用设喻形象与喻意对比以及习语的来源,用以研究习语的文化内涵。这将对到位的理解、恰当的运用动物词汇并真正领悟习语中的动物文化大有助益。     

论体育人的文化

在社会主义现代化与体育事业蓬勃发展的今天,体育文化人概念的提出,在理论上具有前瞻性,在实践上具有可操作性.它确立了人的主体地位,强调了人的全面发展,突出了体育的教育功能.体育人的文化是体育与德育、智育、美育的有机结合.做体育文化人不仅仅是体育工作者的个人修养准则,它应该成为高校体育院校的人才培养模式,使体育人成为具有丰富的知识底蕴和良好的人文素养的社会有用之才.     

从主观文化类同到文化利用——科学之于基督教与科学之于儒学的共同特征

当科学作为外来文化与基督教和儒学分别相遇时,基督教和儒学的捍卫者采取的反应十分相似：无论是基督教父的“希腊哲学源自《旧约》”说还是中国儒士的“西学中源”说都是先让科学“认祖归宗”,这在客观上确立了科学在本已文化中的地位;基督教父的“婢女传统”和中国儒士的“中体西用”说则进一步限定了科学与本己文化之间的从属关系。从“希腊哲学源自《旧约》”说和“西学中源”说到“婢女传统”和“中体西用”说,反映了一个从主观文化类同到文化利用的文化交流过程。     

红山诸文化"神鸟"崇拜与萨满"鸟神"

鸟崇拜是人类原始图腾崇拜的普遍形式.距今8000～5000年的红山诸文化曾经有过十分发达的“神鸟“崇拜.在考古发现的遗物中有许多各类鸟形纹样、鸟形雕塑和以石、陶、泥、木、玉等材质制成的鸟形器和佩饰物.鸟是北方诸族萨满的主神或主要保护神,在北方古代游牧民族中鸟崇拜和鸟祖神话十分流行.红山诸文化的“神鸟“崇拜与北方民族的“鸟神“崇拜,特别是萨满“鸟神“有诸多相似之处.     

理工科学生英语口语现状分析及对策

着力从客观方面总结并分析理工科学生张不开口说英语的主要原因,提出语言本身如词汇、语音及语调、语法与语用等方面的应对策略,旨在有目的地提高大学英语教学。     

休闲文化与信息化对公共图书馆建设的影响

随着社会生活的变化和科学技术的发展,公共图书馆面临着许多新问题。本文通过对休闲文化和信息化对公共图书馆的影响的分析,对公共图书馆的建设进行了初步探讨。