High molecular transport proteins for JH-III in insect hemolymph

Summary Using two independent techniques, ultracentrifugation in a KBr-gradient, and native pore polyacrylamide gel electrophoresis in combination with [³H]-epoxyfarnesyldiazoacetate photoaffinity labeling, we showed that in the hemolymph ofPeriplaneta americana, and probably also inLeptinotarsa decemlineata JH-III binds to the lipophorin, whereas inLocusta migratoria JH-III binds to a different protein.     

重组人甲状旁腺激素(1-34)在大肠杆菌中的表达与纯化

将化学合成的rhPTH(1-34)基因用PCR扩增后,克隆至表达载体pET-35b( ),使rhPTH(1—34)融合于纤维素结合结构域(CBDdos)的羧基端,并得到高效表达．融合蛋白经纤维素树脂亲和层析纯化后,经Factor Xa裂解释放出rhPTH(1-34),再通过纤维素树脂亲和层析、C4反向高效液相色谱纯化得到rhPTH(1-34)纯品．每升培养液可获取3mg高纯度的rhPTH(1-34)．经质谱测定,所得样品的分子量为4117．0Da,与rhPTH(1—34)理论分子量一致．     

浅析商标反向假冒--我国商标立法缺失及对策

将别人附贴在商品上的商标撤掉,再换上自己的商标出售该商品的行为称为"商标反向假冒".该行为侵犯商标权人的商标与商品的不可分割权,损害商标权人的长远利益,属商标侵权行为.基于我国<商标法>及<商标法实施细则>在此方面的缺失,我们应借鉴国外禁止商标反向假冒立法之先进经验,把"商标反向假冒"明确纳入立法范畴.     

Binding of estradiol to synaptosomal mitochondria: physiological significance

The subsynaptosomal distribution and specific binding of 17beta-estradiol in vitro to mitochondria isolated from presynaptic nerve endings of female rat brain were examined. 17Beta-estradiol is (i) distributed unequally in synaptosomes and mitochondria posses the highest capacity to bind estradiol with respect to the available amount of the hormone. (ii) Estradiol binds specifically to isolated synaptosomal mitochondria. A Michaelis-Menten plot of specific binding was sigmoidal within a concentration range of 0.1-5 nM of added estradiol, with a saturation plateau at 3 nM. Binding of higher estradiol concentrations demonstrated an exponential Michaelis-Menten plot, indicating non-specific binding to mitochondria. Vmax and Km for the sigmoidal-shape range were estimated as 46 +/- 6 fmol of estradiol/mg of mitochondrial proteins and 0.46 +/- 0.07 nM free estradiol respectively. (iii) Estradiol binding is not affected by the removal of ovaries. The results show that inhibition of Na-dependent Ca2+ efflux from mitochondria by estradiol occurs according to an affinity change of the translocator for Na+, at the same estradiol concentrations that show specific binding to mitochondrial membranes. These data imply that physiological concentrations of estradiol, acting on mitochondrial membrane properties, extragenomically modulate the mitochondrial, and consequently the synaptosomal content of Ca2+, and in that way exert a significant change in nerve cell homeostasis.     

一种基于网格的数据集成服务系统分析

网格技术越来越多的应用于仿真领域中。仿真程序包含很多数据密集型应用,它们需要使用大量分布的数据库资源。如何访问和操作这些异构的数据库资源是应用程序的关键问题。在利用现有的访问网格中异构数据源方法的基础上,提出了基于网格服务的异构数据源集成体系结构,可以在应用过程中动态绑定数据源;同时在选择数据服务的时候采用基于Qos的选择模型,可以有效的避免服务失效的现象。     

驾驶困倦预警系统中眼部状态识别的研究

为判断驾驶员是否处于疲劳驾驶状态,通过对人脸正面图像中的眼部状态的研究,提出了一种判断算法.首先基于灰度投影曲线和人脸几何特点提取驾驶员的眼部区域,然后利用事先提取的驾驶员睁眼时的瞳孔区域模板在该区域进行匹配,通过匹配初步判断出驾驶员是否属于疲劳驾驶.实验结果表明,该算法简单高效,在满足一定外部条件下,误判率较低.     

图书馆管理手段的探讨

论述了图书馆管理中期刊装订的必要性以及期刊流通的必然性,分析了期刊装订与期刊流通的辨证关系,确定了期刊装订与期刊流通在期刊工作中的重要地位.     

A Statistical Method for Analysis of Technical Data of a Badminton Match Based on 2-D Seriate Images

The use of computer vision technology to collect and analyze statistics during badminton matches or training sessions can be expected to provide valuable information to help coaches to determine which tactics should be used by a player in a given game or to improve the player＇s tactical training. A method based on 2-D seriate images by which statistical data of a badminton match can be obtained is presented. Image capture and analysis were performed synchronously using a multithreading technique. The regions of movement in the images were detected using a temporal difference method, and the trajectories of the movement regions were analyzed using sedate images. The shuttlecock trajectory was extracted from all detected trajectories using various characteristic parameters. The stroke type was determined by comparing the shuttlecock trajectory data with a set of stroke definition data. The algorithm was tested at a training center, and the results were compared with baseline data obtained by expert visual inspection using four video samples, which included approximately 10 000 frames. The shuttlecock trajectory and stroke type were detected correctly in almost 100% of the analyzed video sequences. The average speed of the automated analysis was approximately 40 frames/s, indicating that the method can be used for real-time analysis during a badminton match. The system is convenient for use by a sports coach.     

从“A像B一样C”式比喻的语义搭配看比喻的构成

在“A像B一样C”式比喻性短语作状语的句子中，C与A、B之间的语义搭配呈现出纷繁复杂的情况。C与A、B之间既可以都构成正常搭配，也可以只与其中一个构成正常搭配，而与另外一个形成超常规搭配。与此相对应，比喻句中本体、喻体的构成也并不完全一致。本文运用认知语言学及转换生成语言理论，结合比喻句中的语义搭配来分析比喻中本体的位置及比喻的不同作用。在划分比喻本体和喻体时，要注意其形象的完整性，要和全句的语义搭配关系、语法结构关系结合起来进行分析。划分的方法和标准也应具体句子具体分析，不可一概而论。     

足球比赛中快速进攻传接球失误的原因分析

对足球比赛中快速进攻传接球失误的原因进行了研究,发现运动员传接球技术掌握程度和传接球的时空把握是影响快速进攻传接球成功的主要因素.为训练及比赛中培养运动员快速传接球的意识,提高足球比赛中传接球的应变能力和成功率提供参考.