Cardiac protective role of a novel erythrocyte-derived depressing factor on rats and its Ca^2＋ mechanism

The cardiac protective role of a novel erythro-cyte-derived depressing factor (EDDF) on spontaneous hy-pertensive rats (SHR), calcium overload (CaO) rats and Wistar rats and its mechanism was evaluated. Mean artery pressure (MAP), heart rate (HR) and LVdp/dtmax were measured by physiological recorder. The effect of EDDF on the Ca2+-ATPase activity in myocardial sarcoplasmic reticu-lum (SR) of CaO rats was determined by inorganic phos-phate assay. Calcium transport in myocytes was measured by 45Ca2+ radioactive isotope measurement. The phosphoryla-tion levels of extracellular signal-regulated protein kinases (ERK1/2) in myocardial tissue of SHR and CaO rats were measured by Western blot method. And the ultrastructures of cardiac muscle cells were observed with the transmission electron microscope. The results indicated that EDDF could significantly decrease MAP, HR and LVdp/dtmax in a dose dependent manner (P < 0.05). It seems that the mechanism might relate with activating the Ca2+-APTase, enhancing the uptake and release of Ca2+ from SR (P < 0.05), decreasing the phosphorylation levels of ERK1/2 of myocytes (P < 0.01) and lightening the ultrastructural lesion of cardiac muscle cells. In CaO rats, the Ca2+-ATPase activity decreased clearly com-pared to control (64.99 7.16 vs 94.48 7.68 nmol·min-1 ·mg-1 protein, P < 0.01), while EDDF (100 mg/mL) could significantly increase the activity (87.93 ?9.54 vs 64.99 ?7.16, P < 0.05, n = 7). Both uptake and release rate of Ca2+ (祄ol 45Ca2+/g protein/min) from myocardial SR of CaO rats re-markably decreased compared to control (32.40 ?2.70 and 15.46 ?1.49 vs 61.09 ?10.89 and 25.47 ?4.29, P < 0.05); EDDF (100 mg/mL) could significantly stimulate their activi-ties (50.48 6.76 and 21.76 2.75 vs 32.40 2.70 and 15.46 1.49, P < 0.05). EDDF could evidently down-regulate the phosphorylation of ERK1/2 in myocardial tissue from SHR and CaO rats (P < 0.01), lighten the ultrastructural lesion of cardiac muscle cells of SHR as well. It is concluded that EDDF seems to play protective roles on both structure and function of heart, which closely related with amelioration of Ca2+ transport and inhibition of Ca2+-MAP kinase pathway.     

NMR study on self-assembled cage complex of hexamethylenetetramine and cucurbit[n]urils

Self-assembled complexes between cage com-pounds cucurbit[n = 5—8]urils and hexamethylenetetramine were studied by using NMR techniques. Experimental results reveal that hexamethylenetetramine can lid cucurbit[5]uril to forming self-assembled capsules in which nothing is encap-sulated yet; the cavity of the cucurbit[7]uril can accommo-date a hexamethylenetetramine molecule to form a self- assembled host-guest inclusion. Moreover, both the cavity interaction of the cucurbit[7]uril with hexamethylenetetra-mine稨Cl and the portal interaction of the dipole carbonyl of the cucurbit[7]uril with hexamethylenetetramine稨Cl lead to form self-assembled capsules in which the hexamethylene-tetramine稨Cl are encapsulated in the hexamethylenetetra-mine稨Cl lidded cucurbit[7]uril. Although the structures of the portal and cavity to cucurbit[5]uril are similar, there is no obvious interaction between decamethylcucurbit[5]uril and hexamethylenetetramine, and also between cucurbit [6]uril or cucurbit[8]uril and hexamethylenetetramine.     

超稳Y-沸石催化合成乙酰乙酸乙酯缩酮

用脱铝超稳Ｙ－沸石作催化剂合成了乙酰乙酸乙酯与乙二醇缩酮和丙二醇缩酮,其产率可达到８０％和９６％,考察了催化剂组成和反应时间对反应的影响规律,发现产物比反应物更易在催化剂表面吸附,对反应机理作了讨论。     

论吕叔湘的语文教育思想

吕叔湘语言思想主要包括：１、语文性质论：“语文”指“语言与文字”,即口头语言与书面语;２、语文教育目的论：提高运用语言方案的技能,养成运用语言文字的良好习惯,达到动用语言文学的高境界;３、语文教学论：以学生练习为主,营造良好的语文环境,教会学生自学。吕先生对语言研究有“业余”的特点,其思想既有作为一个旁观者的冷静观察和思考,也不免有重复前人成说、缺少系统研究等特点。     

嫦娥一号的奔月日记

北京时间2007年10月24日18时05分，长征三号甲火箭托举着我国第一颗月球探测卫星——嫦娥一号在西昌卫星发射中心，从三号甲塔架点火发射。11月5日上午11时，嫦娥一号顺利被月球“捕获”成为一颗绕月卫星。在经过三次近月制动后，嫦娥一号进入开展科学探测的工程轨道，随后将进行为期一年的对越科学探测。这是探月三部曲的第一步，也是中国空间探测历史上的新的里程碑。本文介绍了嫦娥一号从发射到2007年11月26日的每一次重要事件。     

[CrMo_6O_(24)H_6]~(3-)掺杂聚苯胺材料的制备及荧光性质

在盐酸介质中,以(NH4)2S2O8为氧化剂,杂多阴离子[CrMo6O24H6]3-为掺杂剂合成出了聚苯胺掺杂材料,用FT-IR,XRD进行了表征,测定了该材料的电导率、常见溶剂中的溶解度及其荧光性质.该材料的电导率为1.4×10-2S·cm-1,最高溶解度可达到0.60mg·mL-1,在以260nm和332nm为激发波长,可分别在425nm和567nm处得到荧光发射峰。     

树的拓扑指标Z_1(G)

分子图的拓扑指标Z1(G)是反映分子物理和化学性质的重要拓扑指标,在n个顶点的树中具有最大Z1(G)和最小Z1(G)的树的基础上确定了n个顶点的树中具有次大,第三大及第四大Z1(G)的树.     

满足左正则性条件的IC富足半群

一个有限半群是满足左正则性条件的IC富足半群当且仅当它是一个幂等元形成左正则带的纯整超富足半群,但满足左正则性条件的无限IC富足半群不都是幂等元形成左正则带的纯整超富足半群.     

多元函数的对称性研究

在n维欧氏空间内,给出了多元函数分别关于点、n-1维超平面对称的充要条件。     

有向线图存在Hamilton圈和Hamilton路的一个充要条件

有向图D的有向线图是以A（D）为顶点集，弧集为{（xy，yz），xy∈A（D），yz∈A（D）}的有向图，用L（D）表示D的有向线图。文章证明了连通有向线图存在Hamilton圈当且仅当它有圈因子；连通有向线图存在Hamilton路当且仅当它有1-路圈因子。