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31.
A. M. Fenyves M. Saxer K. Spanel-Borowski 《Cellular and molecular life sciences : CMLS》1994,50(2):99-104
Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently. 相似文献
32.
B. Streit 《Cellular and molecular life sciences : CMLS》1995,51(5):425-436
The goal of this article is twofold: 1) It aims at providing an overview on some major results obtained from energy flow studies in individuals, populations, and communities, and 2) it will also focus on major mechanisms explaining community structures. The basis for any biological community to survive and establish a certain population density is on the one hand energy fixation by primary producers together with adequate nutrient supply and the transfer of energy between trophic levels (bottom-up effect). On the other hand, predator pressures may strongly control prey population densities one or more trophic levels below (top-down effect). Other interpopulation effects include competition, chemical interactions and evolutionary genetic processes, which further interact and result in the specific structuring of any community with respect to species composition and population sizes. 相似文献
33.
本文合成了通式为REL3(RE3+=Pr3+、Sm3+、Tb3+,,L=对茴香酸根)的二元配合物.用元素分析、电导、IR、UV、TGDTA等对其结构和性质进行了表征.该类配合物属桥式双齿配位 相似文献
34.
以满江红鱼腥藻(Aac)提取总DNA为模板,通过PCR技术,扩增得到其谷氨酰胺合成酶基因(glnA)上游区.经酶切得到409bp的片段,并将其连接到pBluescriptⅡks+上测序.将测序结果与Anabaena7120调控序列比较,有982%的同源性.在上游调控中也具有niflike和E.colilike启动子,值得注意的是,调节蛋白VF1的结合位点正好位于niflike启动子上.所克隆的片段不但对蓝藻固氮、泌氨的基因调控研究具有意义,也对表达载体的构建具有实用价值. 相似文献
35.
Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression
vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine
deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence
analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide
by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank. 相似文献
36.
《科学通报(英文版)》1998,43(5):363-363
During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade. 相似文献
37.
38.
The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and
3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned
into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd,
and the expressed amount reached maxium in 4 h with IPTG induction.
Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee
Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938) 相似文献
39.
对采用注射法导入外源基因的棉铃,在激素种类、不同生态条件、结铃部位、结铃性、整枝方式等方面进行分析,探讨了导入棉铃落铃机理和提高导入棉铃成铃率的技术途径。 相似文献
40.
万士梅 《复旦学报(自然科学版)》1998,37(4):450-454
通过微弹轰击获得的含有bar基因的转基因燕麦植株自交产生转基因后代。利用PCR方法检测bar基因在后代中的传递情况。 相似文献