Gene product expression of cyclin D<Subscript>2</Subscript> and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy

The current study was to investigate mRNA expression of cyclin D₂ and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy. Cultured cardiac myocytes (CM) and fibroblasts (FC) obtained from 1-day-old Sparague-Dawley rats were used in this study. We have determined (1) hyperplasia by cell growth curve and fluorescence activated cell sorting (FACS); and (2) ultrastructure by electron microscope observation; and (3) expresions of cyclin D₂ mRNA and p16 mRNA by using in situ hybridization and image analysis. The results were shown (1) Results of cell growth curve and FACS analysis showed CM could proliferate in the first 3 cultured days (4 days in postnatal development). But the ability decreased quickly, concomitant with the differentiation. (2) The ultrastructure of CM showed the large amount of myofilaments and mitochondrion and FC showed moderate amount of rough endoplasmic reticulum. (3) The expression of cyclin D₂ mRNA in 3&amp;#8722;, 4&amp;#8722;, 5&amp;#8722;day CM group was 0.89 times (p&amp;lt;0.05), 0.80 times (p&amp;lt;0.05) and 0.56 times (p&amp;lt;0.01) of that in 1-day group respectively. P16 mRNA in 2&amp;#8722;, 3&amp;#8722;, 4&amp;#8722;, 5&amp;#8722;day CM group were 1.63 times (p&amp;lt;0.01), 1.72 times (p&amp;lt;0.01), 1.99 times (p&amp;lt;0.01) and 2.84 times (p&amp;lt;0.01) of that in 1&amp;#8722;day group respectively. It can be concluded that cultured neonatal rat cardiac myocytes could proliferate during the first 3 cultured days, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 have the key roles during the transition from myocyte hyperplasia to hypertrophy. Biography: Zhang Yu-xia (1974-), female, Master, research direction: cardiovascular pathology.     

关于p-adic变量函数的微分

给出了p-adic变量p-adic值函数及p-adic变量实值函数的导数定义,将黎曼积分中下列公式推广到p-adic积分中去(1)有限增量公式;(2)积分变量替换公式;(3)微分链导法则.     

序列空间l_(p)的两个提升性质

证明了Banach空间序列{E_n}的l_(p)乘积l_(p)(E_n)的连续对偶空间为m_(p)(E_n~*),l_(p)(E_n)为r-凸空间当且仅当l_(p)为r-凸空间(0相似文献   

数坛怪杰爱尔特希

匈牙利数学家爱尔特希(Erdos,P.)是世界沃尔夫数学大奖得主之一.本文仅从几个侧面,介绍其数学生涯,评述其数学思想、工作特色.     

变p值二叉树形冲突分解算法分析

&nbsp;提出了一种二叉树形冲突分解的改进算法,通过对随机争用多址访问系统中的信息分组冲突分解的研究,给出了冲突信息分组平均分解次数的解析式和系统吞吐量的解析式,仿真实验表明,仿真实现结果与理论值相吻合.     

Functional and physical communication between oncoproteins and tumor suppressors

The discovery of oncogenes (c-onc&amp;#8217;s) and tumor suppressors (TS&amp;#8217;s) has led to the concept that cancer arises from defects in each of these classes of genes or their products. More recently, it has been appreciated that c-onc and TS proteins often affect one another&amp;#8217;s functions. Within this context, I review the two classical TS&amp;#8217;s, p53 and the retinoblastoma protein, and the consequences of their inactivation. The various forms of genomic instability (GI) that underly the high mutation rates of transformed cells are then discussed. Particular emphasis is placed upon the concept that GI is not only an integral part of the transformed state but is a prerequisite. Increased oxidative DNA damage, and/or an inabiliy to repair it, can lead to GI. The review then discusses recent observations showing that loss of the TS protein peroxiredoxin 1 (prdx1) and increased expression of the c-onc protein c-Myc, each leads to increased oxidative DNA damage. The critical nature of the c-onc-TS interaction is underscored by that occurring between prdx1 and c-Myc, with the former protein regulating the production of DNA-damaging reactive oxygen species by the latter. The intimate association between these proteins and others serves as a paradigm for the exquisite balancing act that c-onc&amp;#8217;s and TS&amp;#8217;s must maintain in order to properly control normal DNA replication and cellular proliferation while simultaneously minimizing the acquisition of potentially neoplastic mutations. Received 10 May 2005; received after revision 3 July 2005; accepted 19 July 2005     

探讨P.Erd(?)s对Bertrand假设的初等证明及对其结果的改进

在原有Bertrand假设的基础上,利用并改进P.Erdos的证明方法,得到了n充分大时比(n,2n]较小的区间(n,n+k](其中n/2相似文献   

几类分裂图的（2，1）-全标号

对与频道分配有关的一种染色问题——（p,1）-全标号进行研究,结果表明,图G的（P,1）-全标号是一个映射厂：y（G）uE（G）-{0,1,…,后},使得：G的任两个相邻的顶点得到不同的整数;G的任两个相邻的边得到不同的整数;任一个点和与它相关联的边得到的整数至少相差P-（P,1）-全标号的跨度是指两个标号差的最大值。图G的（P,1）．全标号的最小跨度叫（P,1）一全标号数,记作A：（G）。根据分裂图的特征,利用穷染法,得到了几类分裂图的（2,1）-全标号数。     

乳腺癌患者p185蛋白的表达

目的探讨p185蛋白对乳腺癌的诊断意义.方法采用免疫组化方法对乳腺癌、乳腺增生患者乳腺组织进行p185蛋白检测.结果 30例乳腺癌组织p185蛋白表达,其中12例阳性,阳性率为40%,26例乳腺增生组织未发现p185蛋白表达.结论乳腺癌与乳腺增生组织p185蛋白的表达有显著差异(P<0.05),因此组织p185蛋白表达在鉴别乳腺癌和乳腺增生方面具有重要意义.     

104Cd核子顺排特征的微观研究

应用微观相互作用玻色子-费米子模型的形式体系, 构造出唯象核芯 两准粒子模型的一个微观实现,微观sdIBM-2 2q.p方案. 基于唯象模型和实现方案, 导出原子核的一个d玻色子从退耦、拆散、占据闯入轨道,直到参加顺排所需的最小能量值;据此讨论了核高自旋态的微观结构及其拆对顺排次序.对104Cd核的计算结果显示微观方案较满意地描述了该核的低自旋态和部分高自旋态;核的一个d玻色子的突然拆对不会引起前后状态的显著改变. 最后,本方案指认:这些高自旋态是构建于ν(h11/2)2和π(g9/2)2上的顺排态;而实验上观测到的81 、82 和83 态则很可能分别属于两准质子顺排态、两准中质子顺排态和基态.