Poly(dimethylsiloxane) based microchip for DNA electrophoresis

A novel poly(dimethylsiloxane)(PDMS) -based microchip for DNA separation through electrophoresis has been developed using a micro-electro-mechanical-system(MEMS) technology. Unlike previous hybrid PDMS microchip, one PDMS film is first created on glass support by pressing method in our microchip. Thus, increased band-broadening phenomena, arising from the material nonuniformity at the walls of microchannel, can be avoided in electrophoresis process. A low-viscosity hydroxypropylmethylcellulose-100 (HPMC-100) is used as the separation medium for fluorescent intercalator-labeled double-stranded DNA (dsDNA) fragments. Mannitol is introduced to PDMS-based microchip as a separation medium additive to enhance separation efficiency. At applied electric field strength of 150 V/cm, excellent separations of the PCR marker could be achieved with an effective separation distance of 25mm .     

Relationship between the exposure dose of high intensity focused ultrasound and the heated necrosis element

Heated necrosis element of tissues is the basis for treating tumor by high intensity focused ultrasound (HIFU), so study on the relationship between the HIFU exposure dose and the heated necrosis element of tissues is of great significance for both HIFU clinical applications and basic research. Using Pennes and Rayleigh formulae the relationship between the exposure dose of an annular focused transducer and the heated necrosis element (I.e. Biological focused region) in a bovine liver in vitro at a depth of 2 cm is simulated in an exposure dose range with radiation sound intensity of 7000～25400 W/cm2 and exposure time of 0～20 s. The theoretical results appear to be in quite good agreement with the experimental ones. It is shown that on the basis of existing theories the heated necrosis element produced by HIFU exposure can be predicted. The deviation of the theory from the experiments under higher exposure dose has also been discussed.     

变系数耦合KdV方程组的自B(a|¨)cklund变换及多重孤立波解

通过齐次平衡原则,得到变系数耦合KdV方程组的一个自Backlund变换.通过自Backlund变换,利用ε-展式法可以完全的得到变速多重孤立波解.作为解释,我们得到了方程的二孤子解.     

露天矿生产的车辆安排

运用运筹学中的目标规划决策,建立露天矿生产车辆安排问题的一个多目标和一个单目标的数学模型,并运用目标规划模型的思想,将复杂的多目标问题转化为单目标线性规划问题,得出了露天矿生产的车辆安排计划。     

离散时变系统迭代域最优学习控制

针对线性时变离散系统，首次提出并研究了迭代域内线性二次型最优迭代学习控制问题。所得到的迭代学习控制方法在迭代域内是保性能最优的，其学习率不需主观选取，学习速度可通过改变性能矩阵参数进行调节。     

非线性中立型延迟微分方程Runge-Kutta方法的稳定性

对Rα,β类非线性中立型延迟微分方程给出了稳定及渐近稳定的充分条件,对于Runge-Kutta方法应用于上述问题得到的数值方法，获得了其稳定及渐近稳定的条件。     

分布式被动传感器网异步采样下的机动目标跟踪

针对分布式被动传感器网的特点，提出了一种异步采样条件下机动目标跟踪方法。该方法采用交互式多模型概率数据互联滤波器跟踪机动目标。为启动滤波器，采用最大似然法估计目标初始状态；为适应异步观测条件，提出了马尔可夫转移概率计算方法。仿真实验表明，在分布式被动传感器网中采用该算法能有效进行机动目标跟踪。     

自动膜片弹簧离合器的模糊-PID控制

自动离合器的起步接合过程控制是电控机械式自动变速器(AMT)开发难点的所在,其对汽车纵向驾驶的舒适性和燃料经济性都有较大影响,文中通过对熟练驾驶员驾驶过程的分析,拟合并优化离合器起步结合过程曲线,提出了改进的模糊-PID控制策略.并采用MATLAB/Simulink仿真软件建立自动离合器模糊-PID控制系统仿真模型,模拟结果显示与常规模糊控制、PID控制相比,所提出的控制策略在控制系统运动追踪特定轨迹时具有鲁棒性和实时性的特点.     

基于综合赋权的风险型多属性决策方法

讨论了属性值是有限区间上的连续型随机变量的风险型多属性决策问题。首先,通过密度函数的变换,研究了风险决策矩阵的规范化问题。基于主客观赋权的思想,建立了一个确定综合权重的模型,并给出了求解此类问题的一种方法。最后,以属性值为区间数的多属性决策问题作为特殊情况,给出了实例。     

非线性中立型延迟微分方程单支θ-方法的稳定性

对求解Rα.β类非线性中立型延迟微分方程的单支θ-方法，证明了如下结论：当1/2≤θ≤1时，单支θ-方法是稳定的；当1/2＜θ≤1时，单支θ-方法是渐近稳定的．