Identification of swelling-activated Cl<Superscript>-</Superscript> current in rabbit cardiac Purkinje cells

The presence and functional role of the swelling-activated Cl^- current (I_Cl(swell)) in rabbit cardiac Purkinje cells was examined using patch-clamp methodology. Extracellular hypotonicity (210 or 135 mOsm) activated an outwardly rectifying, time-independent current with a reversal potential close to the calculated Cl^- equilibrium potential (E_Cl). The magnitude of this current was related to tonicity of the superfusate. The current was blocked by 0.5 mM 4,4-diisothiocyanostilbene-2,2-disulfonic acid (DIDS). These features are comparable to those of I_Cl(swell) found in sinoatrial nodal, atrial, and ventricular myocytes. I_Cl(swell) activation at 210 and 135 mOsm depolarized the resting membrane potential with 6 and 10 mV and shortened the action potential by 18 and 33%, respectively. DIDS partially reversed I_Cl(swell)-induced action potential changes. We conclude that I_Cl(swell) is present in Purkinje cells and its activation leads to action potential shortening and resting membrane potential depolarization, both of which can promote the development of reentrant arrhythmias.Received 20 January 2004; received after revision 17 February 2004; accepted 25 February 2004     

FK506 sensitizes mammalian cells to high osmolarity by modulating p38 MAP kinase activation

The immunosuppressants tacrolimus (FK506) and cyclosporin A (CsA) have increased the survival rates in organ transplantation. Both drugs inhibit the protein phosphatase calcineurin (CaN) in activated T cells, exhibiting similar side-effects. Diabetes is observed more often in FK506 than CsA therapy, probably due to inhibition of new molecular targets other than CaN. We studied FK506 toxicity in mammalian cells. FK506, but not CsA, regulated p38 activation by osmotic stress, and decreased viability in osmostressed cells. In addition, FK506 treatment strongly increased the phosphorylation of the eukaryotic initiation factor-2a (eIF-2a) subunit. eIF-2a phosphorylation, p38 inhibition and cell lethality were relieved by addition of excess amino acids to the medium, suggesting that amino acid availability mediated FK506 toxicity. Therefore, these FK506-dependent responses could be relevant to the non-therapeutic effects of FK506 therapy.Received 16 October 2003; received after revision 8 January 2004; accepted 14 January 2004     

复合式氧化沟处理城市废水运行条件的控制研究

采用复合式氧化沟工艺处理城市污水,并对系统的运行条件进行了探讨.实验结果表明:当水停留时间HRT≥30 h,COD、BOD5和氨氮的去除率保持在96%、97%和93%以上,当HRT分别为20 h和10 h时,COD、BOD5和氨氮分别为90%、94%、87%和86%、92%、83%;重点考察在HRT为20 h时,COD的浓度范围从125.3~492 mg/L,氨氮的浓度范围为20~32.91 mg/L,但是它们的去除率维持在90%以上,系统对污染物有更高更稳定的去除效果,而且系统对冲击负荷也有很强的适应性.     

The experimental study of interaction between shock wave and turbulence

The interaction between shock wave and turbulence has been studied in supersonic turbulent mix layer wind tunnel. The interaction between oblique shock wave and turbulent boundary layer and the influence of large vortex in mix layer on oblique shock wave have been observed by NPLS technique. From NPLS image, not only complex flow structure is observed but also time-dependent supersonic flow visualization is realized. The mechanism of interaction between shock wave and turbulence is discussed based on high quality NPLS image.     

Reliability Comparison Between Parallel and Cold-standby Two-unit System Under Poisson Shock

This paper investigates the comparison problem of the reliability index between a parallel and a cold-standby system,both of which are consisting of two identical units.On the contrary to the general intuitive result,we proved that,under the condition that the system is shocked by a Poisson stream,the life time of the parallel system is longer than that of the cold-standby one in the sense of probability.     

Study on the shock absorbing technique of high speed railway bridges

Based on the idea of "bearing function separation", a structural member called shock absorber that makes use of its plastic deformation is presented for reducing the seismic response of the bridge. The design criterion for matching material stress, strain and earthquake fortification aim, is also given. The analysis results show that the high speed railway box girder with the absorber in this paper has great reduction effect in seismic response of the bridge piers.     

NaCl和CHS对滨藜幼苗的渗透胁迫效应

在短时间内，用ＣＨＳ、ＣＨＳ并在根际加压、ＮａＣｌ溶液、ＮａＣｌ溶液并在根际加压处理滨藜幼苗。结果表明ＮａＣｌ与ＣＨＳ对地上部发的渗透胁迫效应可抑制滨藜幼苗的生长，还可减少叶蒸腾率、增加叶细胞质膜透性。     

实现准分子的一种新方法

本文较详细地介绍了什么是准分子,准分子体系的特征及当前准分子的类型.文中另一部分是通过实验,详细地介绍了一种实现准分子的新方法——激波激发.在546 nm到577 nm波长范围内观察到ArO~*准分子连续辐射,通过分析,提出ArO~*准分子的动力学过程.     

次血红素六肽对纳米金诱导线虫热激损伤的保护作用

通过考察Au纳米球壳对野生型秀丽隐杆线虫的光热转换作用, 建立一种新型的秀丽线虫热激损伤模型. 实验结果表明, 在纳米金浓度为30 μmol/L, 红外光强为3 W/cm2, 照射8 min的热激条件下加入次血红素六肽对线虫有保护作用.