MGB probe assay for rapid detection of mtDNAl1778 mutation in the Chinese LHON patients by real-time PCR

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.     

全混洗光互连的矩阵处理与研究

引入矩阵的方法分析处理全混洗互连变换，用矩阵方程形式直观地表达了一些已知的，以及一些新的全混洗互连性质，并阐明了其物理意义，同时提出了全混洗互连、逆全混洗互连的光学实现方法。     

从断层岩中方解石的{01■2}双晶及主晶光轴探讨小关冲断层的主应力方位

本文根据特纳提出的方解石双晶纹动力学分析原理及方法,利用宝兴龙门山北东向断裂带小关冲断层岩中的方解石光轴及{01■2}双晶,建立了主压应力轴σ_1(C轴)、主张应力轴σ_3(T轴)方位,得出的平均方位是,σ_1 127°∠39°,σ_3 309°∠47°,和野外地质调查结果基本吻合。     

基于多元线性逆滤波器的视顶盖神经元集群亮度信息解码研究

通过解码鸽子视顶盖(the optic tectum,OT)神经元集群亮度信息的方法,研究了OT区神经元集群编码亮度信息的神经机制。首先设计了具有瞬态闪变特性的亮度视觉刺激模式,采用微电极阵列记录了多通道的锋电位(spike)发放序列;然后提取了神经元集群的spike发放率特征,构造多元线性逆滤波器解码视觉刺激亮度;最后采用互相关和信息论的方法对解码结果进行了分析。结果表明:采用的多元线性逆滤波器有效解码了OT区神经元集群编码的亮度信息。通过对解码参数变化与解码精度的分析可知神经元集群数目13个、bin的宽度5 ms、刺激后15 ms、刺激后持续时间35 ms具有最高的解码精度。通过神经元集群中逆滤波器形态的分析发现,单神经元在亮度信息的编码过程中受周围神经元的动态调制。通过对解码精度与刺激闪变频率关系的分析,发现在刺激闪变频率19 Hz到53 Hz的范围内,重建质量较好,在刺激闪变频率为33.5 Hz时,重建质量达到最优。     

毛细管电泳-光纤光谱联用技术检测结晶紫

开发新型毛细管电泳-光纤光谱联用技术并应用于结晶紫的快速检测。以结晶紫为对象,考察了电泳和溶液条件对结晶紫的分离分析信号的影响。结果表明,检测结晶紫的最佳条件为:流动相是0.1 mol/L磷酸缓冲溶液(pH 4.0),检测波长590 nm,分离电压3 kV,进样时间60 s.在最佳条件下,吸光度与0.8~10μg/mL结晶紫呈现良好的线性关系,相关系数为0.9954,检出限为0.8μg/mL.方法成功地应用于水体中结晶紫的快速检测。     

Growth, structural, optical, thermal and dielectric properties of a novel semi-organic nonline ar optical crystal: Dichloro-diglycine zinc II

Dichloro-diglycine zinc II(DCDGZ II),a semi-organic nonlinear optical material has been synthesized and single crystals were grown from the aqueous solution up to dimensions 20×10×3 mm3.The title compound,DCDGZ II(C4H10Cl2N2O4Zn H2O) crystallizes into monoclinic structure with the space group of C2/c.The unit-cell parameters were found to be a=14.4191(7),b=6.9180(2),c=12.9452(6) and Z=4.In the crystal structure,DCDGZ II layer is building up alternatingly with layers of water in which the zinc ions lie on a twofold axis.Theoretical calculations for polarizability,which are useful for device fabrication were made using Clausius–Mosotti equation and Penn analysis and the results were compared.Fourier transform infrared(FTIR) spectroscopic studies were performed for the identification of the different functional groups presented in the compound.The UV–vis–NIR absorption spectrum reveals that the lower UV cut-off wavelength is 240 nm.The optical band gap of the crystal was estimated as 2.2 eV.The surface morphology,thermal behaviour,dielectric properties have been studied using SEM,TG/DTA and LCR HITESTER analyzer.The nonlinear optical property of the crystal was also confirmed using Kurtz powder technique.     

采用光纤网络的工业多媒体通信研究

讨论了在光纤网络环境下进行多媒体应用的若干问题。根据实际要求,着重讨论了在工业局域网上运行的,对工业现场进行动态实时监控的新型多媒体监控系统,这套系统包括以图像采集卡为核心的视频处理部分,实现网络传输的通信部分以及系统总控软件。     

光网络中的全光上下话路(OADM)技术

对目前的OADM方案进行了综述,归纳为4类形式,对各类OADM的结构和主要性能做了介绍和评估,并对2种OADM的结构进行了实验研究,最后结合一个OADM的实例做了说明。     

基于全温范围的多通道光纤陀螺自动检测系统研究

介绍了一种基于全温范围的多通道光纤陀螺自动检测系统的方案,给出了系统的硬件环境设计思想, 进行了多路陀螺测试数据采集模块、环境实验机控制模块、自动转台控制模块、测试报告打印模块的设计和 实现。本系统可实现全温范围内多路陀螺同时测试,提高了光纤陀螺的测试效率,可为光纤陀螺工程化服 务。     

旋转中心偏移对反射光调制强度的影响

为了研究新型的旋转中心偏移传感器,在Gauss光束分析法的基础上,提出了漫反射强度调制型光纤传感器测量旋转中心偏移的计算模型。该模型从数学上描述了旋转中心偏移和反射光强度调制之间的关系,定量地得出了光纤束结构参数对传感器灵敏度和线性范围的影响。建立了高精度旋转中心偏移测量实验系统,对模型进行了实验验证。在旋转中心偏移较小的情况下,模型与实验结果相符。