计算力学量平均值的一种方法

论述了量子力学中的海尔曼-费曼定理的内容和应用范围，重点阐明应用海尔曼-费曼定理求力学量平均值的方法，并探讨了粒子在中心力场中的运动．     

关于严格循环权位移的一个问题

给出了一个内射单侧权位移,其权序列空间H~2(β)≠H~∞(β),但对任意φ(z)属于H~∝(β),其部分和序列按算子范数收敛于φ(z).从而否定回答了Shields在文献[1]中的第12问题.     

Expression and Characterization of HGV E2 cDNA in Pichia pastoris

A 559 base pair fragment of cDNA locating at the putative E2 region of GBV-C/HGV was in-serted into Pichia pastoris expression vector pPICgK in the reading frame of α-factor secreting signal pep-tide. The recombinant expression plasmid pPIC9K-E2 was introduced into P. pastoris GSll5 with electro-poration and recombined with the host genome by homological recombination. The His+Mut+ recombinantyeasts were selected and cultivated in the BMMY medium. After 3 days induction with 0. 5% methanol,the target protein(E2) accumulated up to 30% of total proteins in the supernatant. The expressed E2 pro-tein was proved possessing antigenicity and high specificity with Western blot and ELISA probed with serafrom the immunized rabbits and the patients infected by GBV-C/HGV.     

关于极值问题的一个注记

本首先把一元函数极值从本质上推广到多元函数极值，进一步给出了以往教材中没有提到的，关于三元函数极值的有效的具体判别方法。     

耦合拟不动点定理

根据耦合拟不动点和上半连续算子的定义，讨论了在一定连续条件下集值混合单调算子的耦合拟不动点存在性问题，证明了，若A是非空弱闭值上半连续增算子，则A存在不动点以及若A是非空弱闭值上半连续混合单调算子，则A存在偶合拟不动点等结论．     

对我国电子竞技运动发展现状的思考

我国电子竞技运动的发展目前还处于初级阶段,没有统一固定的专业组织,没有规范有序的竞赛市场,广大电子竞技爱好者的运动水平难以获得大的提高. 要想改变现状,跟上世界电子竞技运动的发展步伐,我们必须创新理念. 首先,获得政府的支持和观众的广泛参与,争取企业的赞助,制定政策,完善赛制,使电子竞技迈上发展的正常轨道;其次,走职业化、产业化道路,探索产业赢利模式,培育和开发市场;第三,博采众长,形成中国电子竞技运动的特色,打造自己的品牌,促进整个信息产业和体育事业的发展.     

扩展乘数法逼近问题

本文建立了扩展乘数法逼近的定量化定理;改进了徐利治与Хлодовский的一些结果。     

Fecundity of the blood-feeding insectRhodnius prolixus increases in successive periods of egg production

The fecundity of the blood-feeding insect,Rhodnius prolixus, was observed to increase in successive periods of egg production, each period being triggered by a single large blood meal. As previously published, the fecundity of mated animals was significantly higher than that of unmated animals for the first period of egg production. For a second period of egg production, fecundity increased significantly in both mated and unmated animals. By the fourth period, fecundity had returned to first-feed values for mated animals, but remained high for unmated animals, and the fecundity of mated and unmated animals was not significantly different. Thus, during successive periods of egg production, the processes which maintain fecundity of unmated animals below that of mated animals are overcome.     

Feller—Trotter算子的局部饱和定理

利用Bajsanski-Bajanic抛物线引理建立Feller-Trotter算子的局部小“ο”饱和定理,进一步地应用所得到结论建立了(C_)类算子半群概率表示的局部饱和定理。     

重组大肠杆菌利用醋酸及乳酸为碳源合成PHB

以廉价的工业副产物醋酸及乳酸为碳源,对产PHB重组大肠杆菌进行培养,考察醋酸及乳酸的添加对重组大肠杆菌生长及PHB产量的影响。将来自Cupriavidusnecator的PHB合成操纵子phaCAB基因簇克隆至pBAD载体,得到产PHB菌株BL21＿pBAD＿phaCAB,以阿拉伯糖为诱导剂,在大肠杆菌中进行重组表达。分别使用LB及M9培养基,对重组菌株BL21＿pBAD＿phaCAB进行培养,研究其生长速度及PHB产量,探索产PHB重组大肠杆菌最适培养基。以添加0.04 g/L乳酸、1.2 g/L乳酸、0.02 g/L醋酸、0.6 g/L醋酸、0.04 g/L乳酸+0.02g/L醋酸、1.2g/L乳酸+0.4g/L醋酸的M9培养基(均含2 g/L葡萄糖)为实验组,以M9培养基(含2g/L葡萄糖)为对照组,考察醋酸及乳酸的添加对重组大肠杆菌生长及PHB产量的影响。分别取第6,12,24和36小时的培养液,分析其葡萄糖、醋酸及乳酸含量的变化。结果表明,低氮型M9培养基更适合产PHB重组大肠杆菌在低糖培养环境中生长。在葡萄糖消耗殆尽后,大肠杆菌能够以醋酸及乳酸为碳源进行代谢,因此在培养基中...