二甲氧基甲烷在酸介质中的电氧化特性

DMM在pH<2.5时会发生水解,本文主要研究了DMM在酸性介质中的循环伏安行为随时间的变化情况,并用电化学方法验证了DMM的水解产物是甲醛和甲醇.     

MDR1, cholesterol certification and cell growth: a comparative study in normal and multidrug-resistant KB cell lines

The product of the MDR1 gene (P-gp) has been implicated in the transport of cholesterol from plasma membrane to endoplasmic reticulum for esterification. In previous studies on leukemia cell lines, we suggested that cholesterol esterification may regulate the rate of cell growth and that the MDR1 gene might be involved in this process by modulating intracellular cholesterol esters levels. To further investigate this matter, the rate of cell growth, cholesterol metabolism, expression of the MDR1 gene, and P-gp activity were compared in KB cell lines displaying differences in expression and function of P-gp (drug-sensitive phenotype versus MDR phenotype). The rate of cell growth correlated with cholesterol esterification in all KB cell lines, whereas the over-expression of MDR1 observed in the MDR cell lines was not always associated with an increased capacity of cells to esterify cholesterol. Two known inhibitors of P-gp activity, progesterone and verapamil, strongly inhibited both cholesterol esterification and cell proliferation in all KB cell lines, but they affected intracellular accumulation of labeled vinblastine only in MDR cell lines. These results further support a role for cholesterol esters in the regulation of cell growth and suggest that the P-gp expressed in MDR KB cells is not involved in the general process leading to cholesterol esterification. Received 14 February 2000; received after revision 10 April 2000; accepted 8 May 2000     

加大实验室的开放力度培养高素质创新人才

实验室开放是目前国内高校实验室改革和发展的大趋势,对提高学生的实践能力、动手能力、创新能力、综合素质和培养高素质创新人才具有非常重要的作用,文中介绍了国外实验室开放情况和国内实验室开放的现状,存在的问题以及西南石油大学实验室开放的措施和取得的成果。其目的是引起各高校的高度重视并制定切实可行的办法提高实验室开放的力度。     

一种新型电池的开发和研究

本文从燃料电池在国内发展的技术状况,各类型燃料电池特性对比,结合我国国情,提出了我国应研究开发熔融碳酸盐燃料电池的建议和设想。     

学术信息交流的崭新模式—开放存取

分析了开放存取的意义和现状,讨论了我国发展开放存取的策略。认为,在我国开放存取建设的进程中,要探索发展规律,科学规划,立足现实,合理整合。     

分布式数据库某些连接方式及实现

本文详细讨论包括ＡＰＩ和ＯＤＢＣ等几种分布式系统数据库的连接方法，并介绍其相应的一些实际应用技巧．     

黄鳝成体性腺组织生殖干细胞的体外培养及鉴定

通过黄鳝成体性腺组织的体外培养,探讨黄鳝成体性腺生殖干细胞体外分离培养与鉴定的方法.分离黄鳝性腺组织进行植块培养,倒置相差显微镜下观察细胞生长状况.利用H.E、油红O染色、碱性磷酸酶(AP)细胞化学染色等方法对培养细胞进行鉴定.黄鳝成体性腺组织原代培养3～5 d,可见组织周围有细胞迁出,并形成生长晕.15 d左右,生长晕直径可达3～3.2 mm,生长晕中的细胞主要包括:成纤维细胞、神经样细胞、类生殖干细胞三种类型.成纤维细胞呈不对称的长梭形,突起短而多,细胞核较浅,核仁明显,有2～3个核仁,是生长晕的主要细胞成分.神经样细胞具有放射丝状细胞突起,油红O染色呈阳性反应.类生殖干细胞呈圆形,单核或多核,核质比大,AP染色呈现出阳性反应.探索了适于黄鳝成体性腺组织体外培养的条件和方法,并对原代培养的细胞进行了初步鉴定,为进一步利用体外培养的黄鳝成体性腺生殖干细胞开展黄鳝性逆转分子机制研究奠定了基础.     

The role of repair in radiobiology

Summary Apart from cancer and mutation induction, radiobiological effects on mammals are mostly attributable to cell death, defined as loss of proliferative capacity. Survival curves relate retention of that capacity to radiation dose, and often manifest a quasi-threshold (shoulder). The shoulder is attributable to an initial mechanism of repair (Q-repair) which is gradually depleted as dose increases. Another form of repair, which is not depleted (P-repair), increases the dose required to deliver an average of one lethal event per cell (dose D₀). Neither form of repair can unambiguously be linked with repair of defects in isolated DNA. An important initial lesion may well be disruption of the complex structural relationship between the DNA, nuclear membrane and associated proteins. One form of P-repair may be restoration of that structural relationship.     

吉林郭勒二号露天矿合理工作线长度的优化

露天矿工作线长度是露天开采设计中的一个重要参数,它直接影响到露天矿的生产能力以及经济效益.考虑到吉林郭勒二号露天矿的煤层赋存条件、生产能力、开采工艺及设备类型、工作帮水平推进速度、内排土运距等因素,采用露天矿SMCAD三维建模软件,对吉林郭勒露天矿进行模拟开采.选取12个不同长度的工作线,每个方案设置21个工程位置,分析了不同工作线长度与生产剥采比、运输费用的变化关系.通过以上分析比较,对工作线长度进行了优化,确定出经济合理的工作线长度.     

Superoxide dismutase activity during the plasmodial life cycle ofPhysarum polycephalum

Summary Superoxide dismutase activity was slow throughout the cell cycle of surface cultures ofPhysarum polycephalum. This activity increased markedly when the organism was induced to spherulate. Glutathione (GSH) and hydrogen peroxide (H₂O₂) concentrations changed very little during the cell cycle. During spherulation GSH decreased; H₂O₂ and the cyanide-resistant respiration of plasmodial homogenates increased.