浅析公司、企业人员受贿罪行为表现

针对新刑法罪名———公司企业人员受贿罪的确定,阐述该罪的特征及行为表现,并进一步提出构成本罪几个关键情节的认定,以助于划清罪与非罪,此罪与彼罪的界限     

Immune responses to DNA vaccines

DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promoter, have been shown to induce protective immune responses to a number of pathogens, including viruses, bacteria and parasites. They have also displayed efficacy in treatment or prevention of cancer, allergic diseases and autoimmunity. Immunologically, DNA vaccines induce a full spectrum of immune responses that include cytolytic T cells, T helper cells and antibodies. The immune response to DNA vaccines can be enhanced by genetic engineering of the antigen to facilitate its presentation to B and T cells. Furthermore, the immune response can be modulated by genetic adjuvants in the form of vectors expressing biologically active determinants or by more traditional adjuvants that facilitate uptake of DNA into cells. The ease of genetic manipulation of DNA vaccines invites their use not only as vaccines but also as research tools for immunologists and microbiologists. Received 26 October 1998; received after revision 3 December 1998; accepted 3 December 1998     

物理实验课程体系改革的探索与建设

结合工作实践,探讨了物理实验教学中存在的问题,提出了物理实验教学的改进模式,论述了高校基础实验室规范化管理的必要性和具体内容。     

谈日语中的称呼表现

语言的价值取向取决于该语言的文化背景，日本人不以辈份、年龄来表示对对方的敬意。在家中，一般以孩子的称呼为基准；在外边，多以对方的职务、职业来称呼，有时在其姓名后加[さん]或[君]。     

关节水平的“共驱动”研究

本文以主动肌和拮抗肌的协同作用机理为依据,从表面肌电(EMG)信号的频率参数和肌肉平均传导速度两个方面,对近年来提出的正常人体神经肌肉系统控制的新理论——“共驱动”概念,在关节水平上进行了探讨和研究,实验结果表明:膝关节的主动肌和拮抗肌在持续恒力收缩下,其表面EMG信号功率谱的归一化中值频率随着时间的增加而同时下降;两路EMG信号的归一化中值频率之间的互相关系数随着收缩力的减小而减小,肘关节的肱二头肌不论是作为主动肌还是拮抗肌,在持续恒力收缩下,由表面EMG信号估计得到的肌肉的平均传导速度均随着收缩时间的增加而下降;肱二头肌作为拮抗肌时,平均传导速度明显高于其作为主动肌时的平均传导速度。     

重组人甲状旁腺激素(1-34)在大肠杆菌中的表达与纯化

将化学合成的rhPTH(1-34)基因用PCR扩增后,克隆至表达载体pET-35b( ),使rhPTH(1—34)融合于纤维素结合结构域(CBDdos)的羧基端,并得到高效表达．融合蛋白经纤维素树脂亲和层析纯化后,经Factor Xa裂解释放出rhPTH(1-34),再通过纤维素树脂亲和层析、C4反向高效液相色谱纯化得到rhPTH(1-34)纯品．每升培养液可获取3mg高纯度的rhPTH(1-34)．经质谱测定,所得样品的分子量为4117．0Da,与rhPTH(1—34)理论分子量一致．     

意义的生成与表达——论梅洛-庞蒂早期意义与表达问题

梅洛-庞蒂通过改造格式塔心理学有关概念,指出意义内在于人类的行为结构,诞生于人类原初的知觉经验;通过对知觉经验的现象学分析,揭示出意义内在于人类的表达活动并与之同时发生.他进一步认为,语言表达活动奠基于又超越了身体表达活动,这一举措强调了知觉经验的首要地位,堵塞了一切纯粹思维、纯粹思想的超越之路,同时,知觉经验世界与语言世界的划分也为他试图沟通这两个领域带来了麻烦.     

Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.     

The molecular mechanisms of congenital hypofibrinogenaemia

Congenital hypofibrinogenaemia is characterized by abnormally low levels of fibrinogen and is usually caused by heterozygous mutations in the fibrinogen chain genes (, and ). However, it does not usually result in a clinically significant condition unless inherited in a homozygous or compound heterozygous state, where it results in a severe bleeding disorder, afibrinogenaemia. Various protein and expression studies have improved our understanding of how mutations causing hypo- and afibrinogenaemia affect secretion of the mature fibrinogen molecule from the hepatocyte. Some mutations can perturb chain assembly as in the 153 Cys Arg case, while others such as the B Leu Arg and the B414 Gly Ser mutations allow intracellular hexamer assembly but inhibit protein secretion. An interesting group of mutations, such as 284 Gly Arg and 375 Arg Trp, not only cause hypofibrinogenaemia but are also associated with liver disease. The nonexpression of these variant chains in plasma fibrinogen is due to retention in the endoplasmic reticulum, which in turn leads to hypofibrinogenaemia.Received 17 December 2003; received after revision 19 January 2004; accepted 21 January 2004