特异性抗体和特异性免疫复合物的检测对结核病的诊断意义

以ＥＬＩＳＡ检测１０８例活动性肺结核、３９例稳定期肺结核、３０例肿瘤、１０１例非结核性肺部疾病患者及２０２例正常人血清的抗结核菌纯蛋白衍生物（ＰＰＤ）ＩｇＧ抗体（简称抗－ＰＰＤ）和循环免疫复合物（ＣＩＣ）中的抗－ＰＰＤＩｇＧ抗体（简称ＣＩＣ－ＰＰＤ）。结果表明，活动性肺结核患者的抗－ＰＰＤ和ＣＩＣ－ＰＰＤ水平明显高于正常人和各疾病组患者的水平（Ｐ＜０．００１），但与其病变范围无关。单一测定活动性肺结核患者的抗－ＰＰＤ，敏感性为８５．２％，特异性为９８．５％；测定ＣＩＣ－ＰＰＤ，敏感性为８６．１％．特异性９６．９％。以两者任一项阳性作为诊断活动性肺结核的标准，则敏感性为９３．５％。此外，对１１例活动性肺结核患者的动态观察表明，随着治疗天数的增加和临床症状的改善，患者的抗－ＰＰＤ和ＣＩＣ－ＰＰＤ水平会逐渐降低，前者于治疗后第２０～２４周降至正常水平，后者则于治疗后第１２～１６周降至正常值。研究结果提示测定患者的抗－ＰＰＤ和ＣＩＣ－ＰＰＤ的水平，可用于结核病诊断，对结核病的疗效判定亦有帮助。     

Structural characteristics and biological functions of the HIV-1 gp120 V3 region

Recent studies demonstrate that the V3 loop of HIV-1 gp120 plays an important role in the attachment of HIV-1 to the target cells. Several amino acids in this domain are involved in the interaction of gp120 with the co-receptors. The V3 loop elicits one of the earliest antiviral antibody responses in HIV-1 infection and has been identified as the principal neutralizing determinant (PND). A subset of antibodies to V3 loop show a broad range of neutralizing activity. Unfortunately, this loop undergoes broad mutation and is one of the hypervariable regions. Mutations of some amino acids in this PND could affect syncytium formation, virus infectivity and neutralization. Knowing the structural characteristics and biological functions of the V3 region could help us to understand mechanism of HIV infection and to develop new strategy against HIV-1. In this review, the structural characteristics, variation and biological functions of the V3 loop as well as immunological responses to the V3 loop are discussed.     

Candidate Multi-Peptide-Vaccine Against Classical Swine Fever Virus Induces Strong Antibody Response with Predefined Specificity

IntroductionClassical swine fever virus(CSFV) is a pestiviruswhich causes significantmortality and morbidity inpigs.An epidemic of CSFV in a high pig densityarea can result in devastating financial losses,because few infected pigs can be effectively cured.In many countries in Europe and Asia,classicalswine fever (CSF) is controlled by vaccinationwith commercially available vaccine strains,suchas the Chinese vaccine strain(C-strain,i.e.,hogcholera lapinized virus) orsome modified-live vir…     

Inhibition of IgM antibody-mediated aggregation ofTrypanosoma gambiense in the presence of complement

This paper deals with the immune reaction betweenTrypanosoma gambiense and monoclonal IgM mouse antibody at equivalence with or without rabbit complement. Antibody-mediated trypanosome clumps formed in the absence of complement, and were readily dissociated by complement to become free. In the presence of complement, on the other hand,T. gambiense were not aggregated by the antibody. Free parasites adhered readily to cultured peritoneal macrophages. Complement-mediated dissociation of the clumped trypanosomes in the equivalence area released a large number of previously bound surface antigens. These antigens were capable of binding again to fresh IgM antibody. Experimental results further indicated that the complement system caused a functional alteration, changing the multivalent nature of the IgM antibody in the immune complex into a univalent one. This phenomenon is of great advantage to the infected host in clearing pathogens in vivo, as it allows more antibodies to attach to trypanosomes and subsequently initiate complement activity.     

谷氨酰胺对杂交瘤细胞生长代谢的影响

研究了不同浓度的谷氨酰胺对分泌抗小细胞肺癌单克隆抗体的2F7杂交瘤细胞生长、葡萄糖消耗、乳酸及氨的生成等代谢过程的影响。在细胞培养箱中,用含不同浓度谷氨酰胺的培养液培养2F7杂交瘤细胞,培养条件为pH 7.2～7.4,温度37℃,5%CO_2、95%空气。实验表明,适宜于2F7杂交瘤细胞生长的谷氨酰胺浓度为2.5 mmol/L,谷氨酰胺浓度与细胞的葡萄糖消耗呈反比,与乳酸生成浓度呈反比,与氨的生成浓度呈正比。     

单克隆抗体亲和层析纯化基因工程人γ-干扰素研究

用抗基因工程人γ－干扰素单克隆抗体（２Ａ１２）细胞株亲和层析从表达人γ－干扰素的大肠杆菌抽提液中纯化经稀释复性后的γ－干扰素。一步纯化后的γ－干扰素含量达９５％以上，蛋白质的比活性达 １．２×１０ｕ／ｇ，收率为７８％。洗脱液用 ０．５ｍｏｌ／ＬＮａＣＩ的 ＰＢＳ溶液，洗脱率达９２．８％。     

鸡ND—IBD二联高免卵黄抗体的研制与应用

应用秦皇岛市流行的IBDV,配合ND弱毒疫苗免疫蛋鸡制备出ND—IBD高免卵黄抗体,经各项检验、初步应用符合治疗和预防ND、IBD的要求,具有特异、快速、成本低、易制备的特点。     

单核增多性李氏杆菌多克隆抗体的制备及纯化

采用家兔背部两侧多点皮内基础注射,静脉加强注射,利用饱和硫酸铵(SAS)盐析和G25分析法制备与纯化抗体,制得高纯度、高滴度(1：2560)的多抗,以此作为检测李氏杆菌的诊断试剂。     

白斑综合征病毒膜蛋白VP124参与病毒的感染

3种白斑综合征病毒膜蛋白(VP39、VP124和VP187)各自特异性抗血清用于体外中和试验,研究它们是否参与病毒的感染,进一步用定量PCR检测中和试验中病毒的感染情况.结果表明,WSSV的感染能够被抗VP124抗体中和,VP124在病毒的感染中有起作用.     

矽肺病人的免疫状态

本文检查了矽肺病人的多种免疫状态,发现患者同围血中T细胞百分率平均值比对照组显著降低,而B细胞百分率呈有意义增高;血清蛋白、r、α_1、α_2球蛋白有随病程进展而增高趋势;血清免疫球蛋白IgG和IgA含量的增高亦与病程相一致,至Ⅱ期矽肺二者含量增高显著,但IgM含量未见明显变化,仅在矽肺合并结核组,IgG、IgA、和IgM含量呈明显增高;患者组类风湿因子(RF)和抗核抗体(ANA)合并阳性率(24.5％)比接尘组(13.3％)为高;血中循环免疫复合物(CIC)阳性检出率较高,但Ⅰ期矽肺组与对照组相比,未呈统计学差别;患者血中总补体(CH_(50))和C_8含量未见明显变化。根据患者的T细胞检出率降低,B细胞增高、体液免疫结果与细胞免疫相一致,均和病程进展而呈现规律性变化,以及自身抗体增高等免疫现象,似与自身免疲性疾病有类同之处。随着杂交瘤技术的广泛应用,联系近年国外关于石棉肺免疫学研究,对T细胞亚群变化进行了讨论,这方面的深入研究,对阐明矽肺发病机理是有意义的。对矽肺免疫的进一步研究,从实验设计上也提出了有益的建议。