寄生于大鳍鳠的杜父鱼驼形线虫的生态研究

杜父鱼驼形线虫的总感染率为10.8%.年平均感染强度为1.5(1～3),相对密度为0.2.该线虫在宿主种群中为聚集分布.水温在16℃左右时.感染率、平均感染强度和相对密度最高,分别为40%,3和0.6;宿主年龄对该线虫的种群数量有明显影响.该线虫种群有明显的季节变化,它使被感染的低龄(1～3)鱼的肥满度平均下降6.3%.     

新疆辣椒寄生线虫鉴定初报

1990～1991年,我们从伊犁、昌吉、阿勒泰、阜康、奎屯、石河子等地共采集了辣椒根及根围土共96个线虫标样,并进行了室内形态鉴定,归属于10个属14个种。     

农杆菌介导的花生抗线虫基因遗传转化体系初步研究

白皮秋花生1012种子萌发3d,获得花生无菌苗,切取子叶、胚轴、子叶柄作为外植体。用农杆菌介导法将外植体侵染20-30分钟转入培养基MS+5mg/L BA+0.5mg/L NAA+100μmol/L AS暗光共培养3d,转至加有280mg/L Cef的培养基P2或P2上诱导芽伸长,长至1-2cm时转至促根培养基MS+0.2mg/L BA+75mg/L Kan+280mg/L Cef上促根,经27天观察有5株再生苗长根。结果表明培养基P2上芽诱导率较高,长势也较好,同时乙酰丁香酮对芽诱导率起促进作用。     

自由生活线虫的研究进展

近年来自由生活线虫作为生物学研究的模式动物日益受到重视,尤其是新新华美小杆线虫因其易保存、生活周期短、基因组小等原因被广泛应用于生物学研究的各个领域.本文就新华美小杆线虫在发育生物学、基因和分子生物学方面与其它后生物的相似性和同源性以及转基因线虫在毒力学方面的应用进行综述,旨在表明新华美小杆线虫将成为理想的模式动物为生命科学研究开辟一个广阔的前景.     

利用食线虫真菌防治植物寄生线虫的研究进展

植物寄生线虫每年给农业、林业造成的损失十分巨大,食线虫真菌是植物寄生线虫生物防治中极其有用的生物资源.综述在利用这类真菌研发线虫生防菌剂中涉及的应用基础研究进展,包括食线虫真菌资源,食线虫真菌侵染线虫的分子机制,生物防治现状,杀线虫毒素概况,以及线虫生防中需要解决的生态学问题.     

Two new species of the genus Paracyatholaimus Micoletzky (Nematoda: Cyatholaimidae) from the Yellow Sea

Two new free-living marine nematode species of Paracyatholaimus Micoletzky, 1923 Paracyatholaimus qingdaoensis sp. nov. and Paracyatholaimus huanghaiensis sp. nov. from the Yellow Sea, China are described. Paracyatholaimus qingdaoensis sp. nov. is characterized by cylindrical body with conical tail; spicules reverse S-shaped, distally pointed; gubernaculum plate, with two cusps at distal end, parallel to the spicules; 10 thick setose precloacal supplements in a 5 + 5 arrangement, anterior group of five supplements located at a protuberance. Paracyatholaimus huanghaiensis sp. nov. is characterized by a cylindrical body with conic-cylindrical tail; slender spicules curved, distally pointed; gubernaculum handle-shaped, expanding distally to a plate, with some sawteeth at distal end; three papillose precloacal supplements, each papilla protruding conically with cuticularized narrow ducts. A key for identification of species of Paracyatholaimus is proposed. http://www.zoobank.org/urn:lsid:zoobank.org:pub:0A8E40E5-AE7A-47DA-B755-61E1D088C1E9     

中国辽宁咽滑刃线虫的形态与分子系统学描述

2018年7月自辽宁省沈阳市枯死华山松(Pinus armandii Franch.)体内分离得到一种非常见的线虫，经光学显微镜初步观察到该线虫在枯死松树内种群单一且数量较多，为研究其与松树枯萎的关系，从形态学和分子生物学特征对该线虫进行系统的描述说明，以确定该线虫的种属，更好地进行线虫的鉴定与分类，并对咽滑刃属线虫的数据库进行补充，便以期为松树枯萎病的相关研究提供理论参考。     

Two new species of Lauratonema (Nematoda: Lauratonematidae) from the intertidal zone of the East China Sea

Two new species of free-living marine nematodes of the genus Lauratonema Gerlach, 1953 from an intertidal sandy beach of the East China Sea are described and illustrated. Lauratonema macrostoma sp. nov. is characterized by a spacious buccal cavity, depth 2.1–2.3 times width; cup-shaped amphids; small blade-like spicules (14–16 µm long, 0.55–0.65 anal or cloacal body diameter [a.b.d.]) and unequal length of outer labial setae and cephalic setae (13–17 µm and 9–12 µm respectively). Lauratonema dongshanense sp. nov. can be distinguished by the small buccal cavity with a strong cuticularized transverse strip structure; cup-shaped amphids; small blade-like spicules (14–15 µm long, 0.58–0.67 a.b.d.), unequal length of outer labial setae and cephalic setae (8–10 µm and 5–7 µm respectively); the presence of a small precloacal papilla. A dichotomous key for the genus Lauratonema is given.

http://zoobank.org/urn:lsid:zoobank.org:pub:B5BA6C15-E72A-4C62-97B4-1823E71A9355     

克线丹颗粒剂对甘薯茎线虫病的防治效果

试验研究了室内、室外不同剂量的克线丹颗粒剂对甘薯茎线虫病的防治效果。室内试验结果表明:各处理中以药土比1∶1200处理防治效果最佳,对甘薯茎线虫的致死率最高,校正死亡率达100%;田间试验结果表明:各处理中以45.0 kg/hm2和37.5 kg/hm2克线丹颗粒剂对甘薯茎线虫病的防治效果最佳,防治效果分别达到了97.68%和91.64%。     

松材线虫病木中真菌的研究

研究了江苏南京和浙江舟山地区松材线虫病木标本,并进行分离培养,得到10多种真菌,其中Ophiostoma ips,Ophiostoma lignicola,Sphaeropsis salicicola,Dothiorella gregaria和Haplosporella minor出现的最多;在Ophiostomaspp.和Cephalosporium zonatum的培养皿中出现大量的线虫。在天牛的蛹室和虫道中有大量的长喙壳真菌存在,并与线虫的关系密切。实验证明Sphaeropsis salicicola,Dothiorella gre-garia、Haplosporella minor能引起严重的松木变色。