ND-EDS二联油乳疫苗免疫对比及田间免疫试验

以自制 ND- EDS二联油苗为主进行免疫试验 ,并以同种二联油苗、ND和 EDS单油苗作对照 ,测定各种疫苗免疫后 ND、EDSHI抗体消涨规律 ,并进行强毒攻击试验。在此基础上 ,用自制二联苗实地免疫产前蛋鸡 2 6 830只 ,进行田间免疫试验。结果表明 :自制二联苗免疫效果良好 ,免疫期为 36 0 d左右 ,免疫期内对 ND和 EDS强毒攻击的保护率分别为 10 0 %和 95.8%     

Epidemic Prediction of Hemorrhagic Fever with Renal Syndrome Using Grey Multivariable Model

ＥｐｉｄｅｍｉｃＰｒｅｄｉｃｔｉｏｎｏｆＨｅｍｏｒｒｈａｇｉｃＦｅｖｅｒｗｉｔｈＲｅｎａｌＳｙｎｄｒｏｍｅＵｓｉｎｇＧｒｅｙＭｕｌｔｉｖａｒｉａｂｌｅＭｏｄｅｌＧＥＮＧＪｉａｎｐｉｎｇ（Ｆｉｒｓｔｔｅａｍ，ＦｏｕｒｔｈＭｉｌｉｔａｒｙＭｅｄｉｃａｌＵ...     

Polymorphisms in genes involved in folate metabolism as maternal risk factors for Down syndrome in China

Objective： To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase （MTHFR）, methionine synthase reductase （MTRR）, the central enzymes in folate metabolism that affects DNA methylation and synthesis, and the risk of Down syndrome in China. Methods： Genomic DNA was isolated from the peripheral lymphocytes of 64 mothers of children with Down syndrome and 70 age matched control subjects. Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR 677C→T, MTRR 66A→G and the relationship between these genotypes and the risk of Down syndrome was analyzed. Results： The results show that the MTHFR 677C→T polymorphism is more prevalent among mothers of children with Down syndrome than among control mothers, with an odds ratio of 3.78 （95% confidence interval （CI）, 1.78-8.47）. In addition, the homozygous MTRR 66A→G polymorphism was independently associated with a 5.2-fold increase in estimated risk （95% CI, 1.90-14.22）. The combined presence of both polymorphisms was associated with a greater risk of Down syndrome than the presence of either alone, with an odds ratio of 6.0 （95% CI, 2.058-17.496）. The two polymorphisms appear to act without a multiplicative interaction. Conclusion： MTHFR and MTRR gene mutation alleles are related to Down syndrome, and CT, TT and GG gene mutation types increase the risk of Down syndrome.     

Fluorescent immunosensor based on fluorescence resonance energy transfer between CdSe/ZnS quantum dots and Au nanorods for PRRSV detection

An ultrasensitive and selective sandwich-type fluorescent immunosensor based on fluorescence resonance energy transfer (FRET) between CdSe/ZnS quantum dots (QDs) and Au nanorods (AuNRs) was developed for the rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV). Modified CdSe/ZnS QDs and AuNRs were bioconjugated with primary antibodies M and GP5, respectively. When the target antigen was present, a well-known sandwich-type form CdSe/ZnS QDs-M antibody-antigen-GP5 antibody-AuNRs was produced, and thus reducing the distance between the QDs and NRs. As a result, FRET occurred and the fluorescence intensity of CdSe/ZnS QDs decreased, and this decrease was used to determine the antigen concentration. Ultrasensitive detection of PRRSV at low concentrations in swine serum was achieved with a limit of detection of 0.55 TCID₅₀/mL and a linear detection range of 10¹–3.5 ?× ?10⁴ TCID₅₀/mL. Therefore, the fluorescent immunosensor is selective and highly sensitive; specifically, it can detect PRRSV at low concentrations in swine serum over a large concentration range. This proposed detection method can facilitate the development of high-performance fluorescent immunosensors for the detection of PRRSV and other antigens.     

艾滋病的流行现状和治疗进展

目的：综述艾滋病的流行现状和治疗进展。方法：检索有关献进行综合分析。结果：艾滋病是HIV引起的改命性慢性传染病，流行形势严峻，各国感染人数不断增加，有的地方已经到了失控的地步。结论：目前的治疗不能根除体内的HIV，高效抗逆转录病毒治疗(HAART)，可产生完全的病毒抑制，显延长病人的生命。     

外感湿热咳嗽的辨证施治探微

湿热咳嗽是临床上常见,而且常常被误诊误治的一种咳嗽,此证与热邪、湿邪、寒邪和气虚、阴虚相关;其病因病机和症状复杂,容易误诊。如果诊断上以苔腻为据;治法以宣降肺气,清热利湿;用千金苇茎汤为主方治疗可获良效。     

西藏地区首例妊高征并发HELLP综合征的诊治体会

HELLP综合征是妊高征的一种特殊形式或并发症，国内仅见个案报道，临床表现极不典型，可严重危害母儿生命，及时诊断处理能取得较好预后，应引起广大临床工作者的重视，以免延误诊治。     

尿β_2－微球蛋白和N－乙酰－B－D氨基葡萄糖苷酶测定与肾小球疾病预后的关系

检测５２例原发性肾小球疾病尿β_２－微球蛋白（β_２－Ｍ）和Ｎ－乙酰－β－Ｄ氨基葡萄糖苷酶（ＮＡＧ），探讨其与肾小球疾病恢复过程与预后关系，并设健康儿对照。结果示尿β_２－Ｍ的与ＮＡＧ均值在肾小球疾病全病程均高于对照组，差异有非常显著性意义，从肾小球疾病组内不同病期看，急性肾炎（ＡＧＮ）不同病期均值随疾病恢复而递减，最后接近正常值；肾病综合征（ＮＳ）缓解初期均值较活动期高，以后逐渐下降，但始终未降至正常。２９例病程４个月内其均值已正常，但仍见波动。从肾小球疾病恢复期测定值看，尿β_２－Ｍ、ＮＡＧ仍未恢复正常者分别为４４％、５４．７％。表明肾小球疾病恢复过程中，尽管临床症状已消失，尿蛋白已阴转，仍有近一半病例肾功能尚未恢复正常。尿β_２－Ｍ的与ＮＡＧ检测可反映肾小管和肾实质受损程度，若尿β_２－Ｍ与ＮＡＧ持续升高，可能提示病情迂延或慢性经过。     

心外膜兴奋传导计算机自动检测装置的研制

心外膜标测是准确定位传导副路径或予激综合症的重要方法。本文叙述作者研制的用计算机标测、显示并画出心外膜传导等时图。该系统具有以下特征,(1)在测量完成后,显示心外膜标测图,(2)以5ms或10ms间隔画出彩色等时图,(3)采用近点加权插值函数作出等时图,(4)由于使用集成电路和微型计算机,可使仪器小型化,从而能搬进手术室。文章提供了系统组成、电路设计及用狗进行实验的结果。