矿质元素、维生素、激素对柱状田头菇菌丝体生长的影响

钾、镁、钙以及铜、锌、锰、铁等矿质元素对柱状田头菇菌丝体生长有明显的促进作用 ,而钠、钼等对菌丝体生长的作用较少 .硫胺素、肌醇、吡哆醇、核黄素、泛酸钙等对菌丝体生长均有不同程度的促进作用 ,其中尤以硫胺素的作用最为显著 .0 .5μg·g- 1的NAA、1.0 μg·g- 1的IAA、0 .2 μg·g- 1的GA3 和 0 .2 5μg·g- 1的 2 .4 -D均能促进该菇菌丝体的生长 ,但 1.0 μg·g- 1的6BA和高浓度的NAA却对生长有阻碍作用     

土曲霉M11液体发酵时茵丝球形态与发酵产酶的关系

在不同的培养条件（如接种量、初始pH、温度、转速、不同碳氮源等）下,对土曲霉M11菌丝球的形态与产酶量的关系进行了深入研究,可以观察到有3种基本的菌丝球形态：丝状体、团块和球形,并且大小与致密度有明显差异．结果表明,即使在不同培养条件下,大小一致、致密的球形菌丝球（直径1～2mm）都具有最高的产酶量,菌丝球的形态与真菌产酶量密切相关．因此,在工业发酵产酶过程中,对菌丝球形态的检测,可以作为一种关键的指标来检测发酵的效果．     

金福菇液体培养条件的优化

采用液体摇瓶培养法,以菌丝体鲜质量为指标从培养温度、起始pH值、摇床转速等方面对金福菇的液体培养条件进行了优化.结果表明,金福菇液体培养适宜的条件为pH值6.0～7.0、培养温度30～32℃、摇床转速160～180 r/min以及250mL三角瓶中装液量100mL.     

深层培养条件下金针菇菌丝生长的营养要求

本文研究了不同碳、氮源其及浓度，培养时间对金针菇２０６４２菌株深层培养下菌丝生长量的影响。结果表明，其最佳碳、氮源分别为玉米粉和黄豆饼粉，浓度分别为３％和１．５％；培养７２小时，菌丝量最多。     

高温平菇菌株对比试验研究

通过对5个高温平菇菌株菌丝生长速度和出菇产量的生物统计学比较,筛选出适合河南省春季栽培的高温优质平菇菌株苏研7号、优伏1号．试验结果同时显示子实体产量和菌丝生长速度无相关性．     

白玉菇液体发酵菌丝体生长和产胞外多糖培养基的优化

白玉菇液体发酵培养基的最优配方可为白玉菇工业扩大培养提供基础资料。以葡萄糖、酵母粉、KH₂PO₄和MgSO₄为主要因素,选择胞外多糖含量和菌丝体生物量为试验指标,采用L₉(3⁴)正交试验进行液体发酵培养基的优化。通过抽滤分离菌丝体,烘干称重,计算菌丝体生物量;用苯酚-硫酸法测定胞外多糖含量。试验测得通过对试验结果进行综合加权评分,确定了白玉菇液体培养基的最优配方为葡萄糖 3%、酵母粉 0.15%、KH₂PO₄ 0.30%、MgSO₄ 0.20%。     

培养条件对柱状田头菇菌丝生长的影响

本文通过不同温度、pH值、CO2浓度、相对湿度、培养料含水量和光照等多种处理，研究了上述培养条件对柱状田头菇[Agrocybe cylindracea(Dc.ex Fr.)Mairde]苗丝生长的影响。结果表明柱状田头菇菌丝体生长的最适温度为25℃；最适的pH值为5．5，适宜范围为4～8；当CO2浓度超过0.18％时菌丝生长明显地受到抑制：最适相对湿度为96％，适宜范围为85％--100％；在培养料的料水比为1：1．4时，菌丝生长最快；黑暗条件下，苗丝体生长快速，强光条件下菌丝体生长将受到一定程度地抑制。     

蛹虫草高产菌丝体和虫草菌素的深层培养工艺优化(英文)

蛹虫草Cordyceps militaris是当前用于功能食品和药物开发上较受国内外关注的真菌之一。本研究通过1次一因素法和正交实验设计优化了蛹虫草产虫草菌素和菌丝体的深层培养工艺。最适合菌丝生长的pH值和温度为6.0和20℃,而最适合虫草素积累的pH值和温度为5.0和26℃。蔗糖、蛋白胨、MgSO4、K2HPO4和NAA分别是最适合虫草素积累的碳源、氮源、无机盐和生长因子;培养基的成分对蛹虫草生物量的影响次序为:蔗糖>蛋白胨>MgSO4·7H2O>K2HPO4>NAA;培养基的成分对蛹虫草产虫草素的影响次序为:蛋白胨>K2HPO4>NAA>MgSO4·7H2O>蔗糖。最适合蛹虫草菌丝生长的培养基配比为4%蔗糖,2.5%蛋白胨,0.05%MgSO4·7H2O,0.15%K2HPO4,2.0mg/l NAA;最适合蛹虫草深层发酵产虫草素的培养基配比为4%蔗糖,1.5%蛋白胨,0.05%MgSO4·7H2O,0.05%K2HPO4,4.0mg/l NAA的培养基;在最佳蛹虫草深层发酵产虫草素的工艺条件下,CM8菌株胞外虫草菌素产量达到961.21 mg/l,胞内虫草菌素的含量达到12.87mg/g;菌丝体的产量为22.97 g/l,本研究得出的结论对深层培养提高蛹虫草虫草菌素和菌丝体的产量有借鉴意义。     

Sclerotial and low aflatoxigenic morphological variants from haploid and diploidAspergillus parasiticus

Summary Serial transfer of mycelial macerates of a wild type, haploid, aflatoxigenic strain ofAspergillus parasiticus in a defined liquid medium resulted in the production of three new morphological classes: a sclerotial form with high aflatoxin production, and two variant forms (fan andfluff) with lowered sporulation, no sclerotia, and attenuated levels of aflatoxin. A genetically marked diploid containing mutant markers for aflatoxin pathway intermediates yielded the same three morphological classes upon serial transfer of macerated mycelia. When these diploid variants were treated with a haploidization agent, and the phenotypes of the resultant segregants scored, a low frequency of colonies producing aflatoxin pathway intermediates was recovered. These genetic data indicate that the structural genes for the aflatoxin pathway are present but somehow attenuated in thefan andfluff strains.This work was supported by a Cooperative Agreement from the U.S. Department of Agriculture, (58-7B30-3-556).     

Properties and characterization of Au3+-adsorption by mycelial waste ofStreptomyces aureofaciences

Mycelial waste of Streptomyces aureofaciences procured from the aureomycin fermentation industry is used as biosorbent for Au³⁺  The properties of Au³⁺ adsorption by the mycelial waste are studied. The results indicate that the optimum pH value of Au³⁺ adsorption is 3.5. The biosorption is a rapid and non-temperature-dependent process. The bio-sorptive capacity with 45.6 mg/g and efficiency with 91.2% are achieved under the conditions of pH 3.5 and 30 ℃ for 45 min, in which the ratio is 50 mg/g dry weight for the concentrations of initial Au³⁺ and the mycelial waste. The Au³⁺ ions adsorbed on the mycelial waste can be eluted. The observation in a transmission electron microscope shows that the Au³⁺ ions can be reduced to Au particles by the mycelial waste and the Au⁰ can become gold crystals with differentforms and sizes. X-ray photoelectron spectroscopy analysis further proves that the Au³⁺ can be reduced to Au⁰ by the mycelial waste.