当前计算机病毒的特点和编制技术介绍

本文介绍了计算机病毒的特征及当前计算机病毒所表现出来的新特点 ,简要说明了编制计算机病毒所使用的新技术 ,最后还简述了防范计算机病毒的基本方法     

单胺氧化酶活性分析在HBV感染者中的应用

目的分析乙肝病毒(HBV)感染者血清单胺氧化酶活性,研究其临床应用价值.方法应用终点比色法检测血清单胺氧化酶(MAO)活性;ELISA法检测乙肝病毒标志物;PCR法检测HBv DNA;常规方法检测ALT.结果 ALT异常组,大三阳HBsAg(+),HBeAg(+),Anti-HBc(+)和小三阳HBsAg(+),Anti-HBe(+),Anti-HBc(+)合并HBV DNA阳性的HBV感染者MAO显著升高,而小三阳并HBV DNA阴性者MAO升高不明显;ALT正常组仅40例大三阳患者MAO升高明显,其他各组MAO大多在正常范围内.结论 HBV感染后,只要有病毒复制,就会破坏肝细胞,引起MAO升高.MAO活性分析对肝脏病情的判断有重要价值.     

小型局域网的计算机病毒防范要点

在分析Windows NT网络、简单对等网络、NetWare网络及Unix/Linux网络等结构特点的基础上，对常见小型局域网提出不同的计算机病毒防范要点。     

沙棘果水溶性多糖Hn的分离纯化与抗病毒研究

沙棘果皮沸水煮提得到粗多糖，经纸层析及气相色谱分析，该粗多糖组成为Ａｒａ，Ｆｕｃ，Ｇａｌ，Ｍａｎ，Ｇｌｃ和ＧａｌＡ，粗多糖经酸性乙醇分级得四个级分，将对柯萨奇病毒Ｂ３有初步抑制作用的ＨＲ３用ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５柱层析进一步分级，得到２个级分Ｈｎ和Ｈａ，Ｈｎ是由Ａｒａ，Ｇａｌ，Ｍａｎ和Ｇｌｃ组成的中性杂多糖，对柯萨奇病毒Ｂ３具有明显的抑制作用。     

颗粒体病毒对小菜蛾种群控制作用的评价

在组建小菜蛾自然种群生命表的基础上，应用种群趋势指数和干扰作用控制指数，评价小菜蛾颗粒体病毒对小菜蛾种群的控制作用．试验结果表明：施用颗粒体病毒制剂后，其种群趋势指数为5．99，干扰作用控制指数为023．即，其下代数量明显下降．可见，小菜蛾颗粒体病毒对小菜蛾种群的控制作用是明显的．     

斜纹夜蛾NPV染病幼虫发育与温度的关系

在不同温度下，以不同剂量的ＳｌＮＰＶ饲食斜纹夜蛾ＳｐｏｄｏｐｔｅｒａｌｉｔｕｒａＦ．幼虫．结果表明，１～６龄染病幼虫的发育起点温分别是１８．７９，１６．２９，１４．１９，１０．８７，８．３９，７．０３℃；１～４龄染病幼虫的发育最适温分别是３２．８５，３３．４８，３４．２２，３１．７５℃．１～６龄染病幼虫的取食最适温分别是２６．５，２５．５７，２７．９０，２７．８２，２９．０１，２８．８５℃．建立了１～６龄染病幼虫取食白菜量的温度依赖模型，死亡速率模型，以及依赖于温度和ＳｌＮＰＶ饲食量的发育速率模型     

一种简便提纯白斑综合症病毒核衣壳的方法

通过人工感染 ,获得具典型白斑综合症的病虾 ,取病虾的鳃和胃组织冰浴匀浆 ,通过 3次冷冻差速离心 ,获得大量较纯的病毒核衣壳 ,其大小为 (5 8～ 75 )nm× (36 0～ 42 0 )nm。     

Tobacco curly shoot virus iso-lated in Yunnan is a distinct species of Begomovirus

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).     

Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells

Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability.