纳洛酮治疗病毒性脑炎30例临床观察

对30例病毒性脑炎昏迷病儿，在常规治疗基础上加用纳洛酮，结果辅用纳洛酮治疗病毒性脑炎的疗效明显优于对照组（P＜0．01）：治疗组与对照组平均住院天数分别为11．3±2．07天，14．2±0．86天（P＜0.05）有显著性差异。     

地高辛掺入法检测HCV RNA含量在肝病诊断中的应用

目的：研究血清HCVRNA含量与HCV感染者肝病程度的关系。方法：应用地高辛掺入法检测血清HCVRNA含量。在PCR过程中将DIG－dUTP掺入到PCR产物中去，再用PCRELISA法检测PCR产物，结合标准参考样本而达定量目的。结果：丙肝后肝硬化组血清HCVRNA含量显著高于丙型肝炎组。结论：地高辛掺入法检测血清HCVRNA含量可以为HCV感染者的病情判断提供科学依据。     

Metabonomic analysis of hepatitis B virus-induced liver failure： identification of potential diagnostic biomarkers by fuzzy support vector machine

Hepatitis B virus （HBV）-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 24 HBV-indueed liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry （GC-MS） to generate metabolite profiles. The 24 patients were further grouped into two classes according to the severity of liver failure. Twenty-five eommensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor （KNN） and fuzzy support vector machine （FSVM） combined with exhaustive search （ES）, were employed to identify a subset of metabolites （biomarkers） that best predict liver failure. Based on the achieved experimental dataset, 93.62% predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyeerie acid, cis-aeonitie acid and citric acid, are identified as potential diagnostic biomarkers.     

SlNPV PK基因的部分序列分析

ＳｌＮＰＶ基因组ＤＮＡ的ＨｉｎｄⅢ－Ｘｂａ片段上含有部分的ＰＫ基因，ＳｌＮＰＶＰＫ的氨基酸序列与ＨａＮＰＶ，ＨｚＮＰＶＳｐｌｉＮＰＶ，ＡｆＮＰＶ，ＡｃＮＩＶ，ＬｄＮＰＶＰＫ氨基酸序列的同源性分别为４５％，４４％，８９％，３７％，３８％和３７％，其上含有蛋白激酶的特征序列ＩＶＨＡＮＤＶＫＬＥＮＶＬ。     

两种抗原不同方法检测猕猴B病毒抗体的初步探讨

分别以人单纯疱疹病毒１型（ＨＳＶ－１）作抗原，采用玻片免疫酶法（ＥＩＡ）和以Ｂ病毒作抗原，采用ＤＩＡｄｏｔ法检测了６０份猕猴血清中的Ｂ病毒抗体。结果ＥＩＡ法检测的阳性率为５％，ＤＩＡｄｏｔ法检测的阳性率为１０％，两者的符合率为９５％。这一结果可为猕猴生产和出口单位提供参考。     

黄瓜花叶病毒的侵染扩散及病变细胞的电镜观察

在电镜下观察黄瓜黄叶病毒的侵染扩散部位发现：病毒粒体入侵细胞及复掣一子 体的扩散同烟草花叶病毒一样是以整体形式向细胞壁靠拢，附着，并通过胞间连丝进入易感细胞，同时还发现少量病毒粒体似有直接穿过胞壁入胞的现象。     

广州天河区手足口病流行病学和临床上的某些特点

自1988年3月至1989年2月,对广州天河区5所幼儿因的1502例婴幼儿进行一年的连续观察,发现手足口病(Hand-Food and Mouth disease,HFMD)62例,发病率为4.13％。发病在5岁以下者占79.09％。发病时间集中在3～7月,呈局部性小流行。首次发现在广州流行的手足口病的病原为柯萨奇A16病毒。皮肤接触感染可能是传染途径之一。口腔粘膜疹在早期常呈细小红斑和瘀点。有些皮疹呈不规则形的灰白色小疱,似烫伤样,无明显红晕。诊断本病应以临床特点为主。一例手足口病与水痘伴发,提示两者之间缺乏交叉免疫能力。