Mitotic regulation of the anaphase-promoting complex

Orderly progression through mitosis is regulated by the anaphase-promoting complex/cyclosome (APC/C), a large multiprotein E3 ubiquitin ligase that targets key mitotic regulators for destruction by the proteasome. APC/C has two activating subunits, Cdc20 and Cdh1. The well-established view is that Cdc20 activates APC/C from the onset of mitosis through the metaphase-anaphase transition, and that Cdh1 does so from anaphase through G1. Recent work, however, indicates that Cdh1 also activates APC/C in early mitosis and that this APC/C pool targets the anaphase inhibitor securin. To prevent premature degradation of securin, the nuclear transport factors Nup98 and Rae1 associate with APC/C^Cdh1-securin complexes. In late metaphase, when all kinetochores are attached to spindle microtubules and the spindle assembly checkpoint is satisfied, Nup98 and Rae1 are released from these complexes, thereby allowing for prompt ubiquitination of securin by APC/C^Cdh1. This, and other mechanisms by which the catalytic activity of APC/C is tightly regulated to ensure proper timing of degradation of each of its mitotic substrates, are highlighted. Received 8 October 2006; received after revision 24 November 2006; accepted 8 January 2007     

洗手液对豌豆根尖细胞的遗传毒性研究

以豌豆根尖细胞为材料,研究不同浓度的洗手液在不同处理时间对豌豆根尖细胞有丝分裂的影响.结果表明:随着洗手液浓度的增加和处理时间的延长,根尖细胞有丝分裂指数下降,在一定的浓度范围内,畸变率升高.并出现了染色体桥、微核、不等分裂等多种分裂异常类型.说明洗手液对豌豆根尖细胞有丝分裂有一定的抑制作用,其具有一定的遗传毒性.     

高速主轴用陶瓷滚动轴承

根据近年来国外高速轴承的研究成果，对混合式陶瓷球轴承的特点、陶瓷球的材料与加工、轴承结构及润滑等进行了详细论述与分析     

差分Painleve方程亚纯解的增长级

利用亚纯函数的Nevanlinna值分布理论的差分模拟,研究了给定的差分Painlev$\\acute{e}$方程I和差分Painlev$\\acute{e}$方程II的超越亚纯解的增长性,得到了一些有意义的结果：在给定的条件下,给出了给定的差分Painlev$\\acute{e}$方程I和差分Painlev$\\acute{e}$方程II的超越亚纯解的增长级的精确估计.     

化纤高速卷绕机锭轴传动控制方法的分析

通过对化纤长丝高速卷绕机锭轴传动方式的原理分析和研究，从不同的角度论述了几种实用的锭轴传动数学模型及其控制方法，为在生产过程中灵活实施并确保恒线速卷绕，提供了理论依据和有效手段，它是实现卷绕机高速化的一项重要技术。     

超细纺锤形CaCO3制备过程研究

在超重力反应器中,Ca(OH)2经碳化反应制得超细纺锤形碳酸钙.采用pH计、XRD、TEM、EDTA络合滴定原位检测了超重力反应沉淀法制备轻质纺锤形碳酸钙的碳化过程特性.结果发现:碳化反应前期,二氧化碳吸收为控制步骤,碳化反应后期转化为Ca(OH)2溶解控制.纺锤形碳酸钙的形成无论是形貌还是晶型都是一个渐变的过程.     

亚硫酸钠、亚硫酸氢钠对小麦幼苗生长及细胞分裂的影响

研究二氧化硫体内衍生物——亚硫酸钠、亚硫酸氢钠混合液对小麦幼苗生长和细胞分裂的影响.结果表明:低浓度(0.1mol/L)的亚硫酸钠、亚硫酸氢钠的混合液对小麦幼苗生长及细胞分裂有促进作用;高浓度(0.2mol/L)的亚硫酸钠、亚硫酸氢钠的混合液抑制小麦幼苗生长及细胞分裂.     

Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells

The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48. The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245?374 and aa 518?646). Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through G1 to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.     

机床三支承主轴刚度分析计算

本文应用最小应变能原理对机床三支承主轴进行刚度分析计算,并对机床三支承主轴和两支承主轴相对刚度分析比较,论证三支承主轴系统结构是提高机床刚度的有效途径。     

超重力反应器中碳化反应参数对碳酸钙产品的影响

结合碳酸钙的成核、生长动力学论述了超重力反应结晶法制备纺锤形碳酸钙过程中主要工艺参数对碳化过程的目标函数之一产品碳酸钙的沉降比体积值的影响,并提出必要的控制条件.同时,将超重力下合成的纺锤形碳酸钙与传统制备方法制得的纺锤形碳酸钙进行了比较,结果表明,其质量要优于传统塔式设备制备的纺锤形碳酸钙.