杜仲组织离体培养研究

以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS KT1 0mg/L NAA0 1～0 5mg/L;MS BA2 0mg/L NAA0 1～0 5mg/L,最佳芽丛增殖培养基为:MS KT2 0mg/L NAA0 1～0 2mg/L;MS BA1 0～2 0mg/L NAA0 1～0 2mg/L;MS KT2 0mg/L IBA0 1～0 2mg/L;MS BA1 0～2 0mg/L IBA0 1～0 2mg/L,单芽增殖培养基:MS KT1 0mg/L NAA0 1mg/L,生根培养基:13MS KT0 1mg/L NAA0 5mg/L.     

Fatty acid metabolism in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis>

Peroxisomes are essential subcellular organelles involved in a variety of metabolic processes. Their importance is underlined by the identification of a large group of inherited diseases in humans in which one or more of the peroxisomal functions are impaired. The yeast Saccharomyces cerevisiae has been used as a model organism to study the functions of peroxisomes. Efficient oxidation of fatty acids does not only require the participation of peroxisomal enzymes but also the active involvement of other gene products. One group of important gene products in this respect includes peroxisomal membrane proteins involved in metabolite transport. This overview discusses the various aspects of fatty acid -oxidation in S. cerevisiae. Addressed are the various enzymes and their particular functions as well as the various transport mechanisms to take up fatty acids into peroxisomes or to export the -oxidation products out of the peroxisome to mitochondria for full oxidation to CO₂ and H₂O.Received 19 February 2003; received after revision 27 March 2003; accepted 27 March 2003     

周期性机械拉伸对人肺上皮细胞增殖及粘弹性的影响

采用FX-4000基底加载系统对体外培养人肺上皮细胞A549施加周期性拉伸应变,通过流式细胞仪和微管吸吮技术,研究了不同波形的周期性机械拉伸对A549细胞的增殖及粘弹性的影响。结果表明:分别采用正弦波和方波,对A549细胞施加15%拉伸应变、频率30次/min周期性机械拉伸4 h,与对照组相比,正弦波拉伸对A549细胞增殖无显著影响,细胞的粘弹性参数k1和k2值有所减小,μ值无统计学差异。方波拉伸在显著抑制A549细胞增殖同时,细胞的粘弹性参数k1,k2和μ值均明显减小。从力学的角度证实了细胞骨架参与细胞生长的过程。     

移动电话射频辐射对机体免疫系统影响的研究进展

从流行病学调查、动物实验和体外研究等方面,综述了近年来国内外学者有关移动电话射频辐射对机体免疫系统影响的研究成果.目前,有关评价电磁场暴露及其危害的流行病学调查依据还很有限,动物实验和体外研究方面的实验结果也缺乏一致性,所以对移动电话射频辐射是否会影响人体健康尚无定论,主要是因为对射频场的作用机理不明确,导致无法制定测试电磁场效应的统一标准.因此,尽早明确射频场的作用机制从而确定流行病学和实验研究的生物指标,是揭示移动电话射频辐射生物学效应的必要途径.     

ISS： Efficient Search Scheme Based on Immune Method in Modern Unstructured Peer-to-Peer Networks

Flooding is the most famous technique for locating contents in unstructured P2P networks. Recently traditional flooding has been replaced by more efficient dynamic query （DQ） and different variants of such algorithms. Dynamic query is a new flooding technique which could estimate a proper time-to-live （TTL） value for a query flooding by estimating the popularity of the searched files, and retrieve sufficient results under controlled flooding range for reducing network traffic. However, all DQ-like search algorithms are ＂blind＂ so that a large amount of redundant messages are caused. In this paper, we proposed a new search scheme, called Immune Search Scheme （ISS）, to cope with this problem. In ISS, an immune systems inspired concept of similarity-governed clone proliferation and mutation for query message movement is applied. Some assistant strategies, that is, shortcuts creation and peer traveling are incorporated into ISS to develop ＂immune memory＂ for improving search performance, which can make ISS not be blind but heuristic.     

板蓝根凝集素对小鼠胸腺淋巴细胞分裂作用的电镜观察

目的：研究板蓝根凝集素引起小鼠胸腺淋巴细胞分袭增殖过程中的超微结构变化。方法：用生物化学方法提取板蓝根凝集素，并测其凝集活性，以0．054mg/kg/d的剂量灌胃，连续30d，胸腺组织经处理后，用Epon812包埋，经超薄切片后，在电镜下观察，结果：与对照组相比，实验组小鼠胸腺淋巴细胞质内的线粒体、核糖体数量均较对照组多，处于分裂前期的细胞数目较对照组多。结论：板蓝根凝集素可能具有促进小鼠胸腺淋巴细胞分裂的作用。     

血淮淋巴细胞计数与小儿先天性心脏病并发症发生的关系探讨

为探讨小儿先天性心脏病并发症的发生与血液淋巴细胞的相互关系 ,笔者将 39例住院的先天性心脏病患儿分为 2组进行比较研究 ,血液淋巴细胞计数百分率≥ 2 0 .3%者为A组 ,≤2 0 .2 %者为B组 .结果表明 :B组与A组比较 ,前者的心力衰竭、心律失常发生率显著升高 (P<0 0 5 ) .提示 :先天性心脏病患儿的血液淋巴细胞降低易并发心力衰竭、心律失常 .     

放线菌酮体外强烈抑制小鼠活化T淋巴细胞CD69表达

目的 :利用淋巴细胞的早期活化标记物CD6 9的表达 ,探讨放线菌酮 (CHX)对T淋巴细胞体外活化的影响 ,以进一步揭示CHX对免疫系统的影响 .方法 :分离小鼠淋巴结细胞 ,与CHX预孵 1h ,加入多克隆刺激剂继续培养 ,总计培养 2 4h后收获细胞 ,进行双色免疫荧光标记 ,以流式细胞术对T细胞的CD6 9分子表达情况进行分析 .结果 :在CHX(终浓度 10 μmol/L)作用下 ,ConA和PDB活化的T细胞CD6 9表达百分率依次为 12 33%± 4 75 %、13 0 7%± 11 0 5 %,与对照组比较均有显著差异 (P <0 0 1) .结论 :CHX(10 μmol/L)对ConA、PDB活化的T淋巴细胞均显示了强烈的抑制效应 .     

杜仲愈伤组织的诱导及增殖效应

以杜仲（Eucommia Umoidues Olive）的幼茎为外植体，接种于附加NAA、2，4-D、KT、6-BA及其组合的MS培养基上，均能产生愈伤组织，但MS 2，4-D的培养基上愈伤组织容易褐化，其他均可正常生长，MS NAA0.5mg/L 6-BA0.5mg/L KT1.0mg/L的培养基是适合愈伤组织的增殖培养的。