人类心房肌细胞的原代培养与鉴定

探索人心房肌细胞的原代培养方法。取心外科手术患者(5/12~57岁,平均6.5岁)常规切除的右心耳,利用酶消化法原代培养心房肌细胞并进行光镜和免疫细胞化学鉴定。结果急性分离的细胞成圆形,1 d后开始贴壁。贴壁的心肌细胞部分聚集成团,成梭形或三角形。随着培养时间延长,细胞逐渐向四周伸展。免疫细胞化学分析显示90%以上的细胞呈横纹肌肌动蛋白染色阳性。说明利用酶消化法成功培养出人心房肌细胞,为深入研究人心房肌细胞基因与功能打下了基础。     

c-fos和CYP17对多囊卵巢综合征(PCOS)患者卵巢颗粒细胞雄激素分泌过多的影响及机制

 为研究多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中c-fos 及CYP17 基因表达的变化,探讨c-fos 及CYP17 基因对PCOS 卵巢颗粒细胞雄激素分泌的影响及可能的作用机制,对拟行IVF/ICSI-ET 的PCOS 患者及非PCOS 患者分为对照组(非PCOS 患者)和PCOS 组(PCOS 患者),两组患者的卵巢颗粒细胞进行体外培养48 h,利用放射免疫法检测细胞上清液中的雌二醇(E2)、孕酮(P)、睾酮(T)的水平;利用2-NBDG 标记葡萄糖检测颗粒细胞的葡萄糖摄取能力;采用免疫蛋白印迹(WesternBlot)法分别评估颗粒细胞中c-fos 及CYP17 基因的表达变化。结果发现,PCOS 组患者与对照组患者相比,颗粒细胞培养上清液中睾酮水平升高(P<0.05)、孕酮水平降低(P<0.05)、雌二醇水平无明显变化(P>0.05),且颗粒细胞对2-NBDG 标记葡萄糖摄取明显降低(P<0.05),提示PCOS 患者卵巢颗粒细胞雄激素分泌异常增高,并存在糖代谢异常。与对照组患者相比,PCOS组患者颗粒细胞CYP17 基因表达升高(P<0.05),c-fos 基因表达降低(P<0.05),提示颗粒细胞c-fos 基因异常低表达,可能降低了对CYP17 基因的抑制作用,使CYP17 高表达,可能是卵巢颗粒细胞雄激素分泌增加的原因之一。     

双酚A类同系物的雌激素效应及对MCF-7细胞的毒性

双酚A(bisphenol A, BPA)及其类似物作为聚碳酸酯的主要合成原料, 一直是环境污染的重要问题, 其环境分布及人体暴露是当今科学研究的热点. 为评估环境中BPA 类物质的毒性效应, 采用MTT 比色法检测了7 种BPA 类同系物对人雌激素受体(estrogen receptor,ER)缺失乳腺癌细胞MCF-7(ER–) 增殖活性的影响. 通过检测细胞乳酸脱氢酶(lactate dehydrogenase, LDH)露出率评估了目标物对MCF-7 细胞膜的损伤, 结果表明, 在0.01～1.00 μmol/L 浓度范围内, 受试化合物对MCF-7 细胞的毒性较小, LDH 少量露出; 而在10 和100 μmol/L 浓度下, 细胞增殖受到显著抑制, 细胞膜通透性显著改变, LDH大量露出. 同时采用ER 基因双杂交酵母实验检验了这几种BPA 类同系物的雌激素效应大小, 通过Logistic 模型对曲线进行了拟合, 并以模型估算的半数透导活性(EC50) 评估了7 种BPA 类同系物的雌激素效应活性, 其大小依次为TDP>BPAF>4Cl-BPA>BPC>BPA>BPE>BPAP. 这说明其雌激素效应机制可能是通过和ER 结合而产生的.     

涡虫成体干细胞研究进展

干细胞研究是当代生命科学研究的重点和热点之一,涡虫因具有结构简单、再生速度快、基因与脊椎动物同源性高等特点而成为研究再生的良好动物模型.涡虫的这种再生能力与其体内具有被称为neoblasts的多能干细胞群有关.文中结合近几年的研究成果从neoblasts与涡虫的再生关系、neoblasts的分子标记及研究方法3个方面进行综述,以促进neoblasts的研究.     

太极拳锻炼对免疫平衡影响及其机制研究进展

通过整理与分析国内外文献,分析了太极拳锻炼影响CD4+/CD8+细胞平衡与T1/T2平衡的作用与机制.研究认为,太极拳锻炼可引起CD4+细胞数量与CD4+/CD8+细胞比值升高,该变化可能与MDC数量的增高促进了Th细胞的分化过程有关;太极拳锻炼可提升Th1细胞数量与Th1/Th2细胞比例以及Ⅰ型细胞因子IFN-γ,IL-2含量与IFN-γ/IL-4比值,诱发T1/T2平衡向T1方向漂移.该变化的机制可能是Treg数量的增高抑制了Th细胞的分化过程,从而降低了IL-4对Th1细胞分化的交互抑制作用.     

T细胞介导炎症与血液肿瘤发生相关性研究进展

慢性炎症是肿瘤发生过程中一个重要因素,越来越多的研究显示T细胞介导炎症与多种肿瘤发生密切相关。本文主要综述近期有关T细胞介导炎症与血液肿瘤发生的相关性及其分子机制的新进展。     

半互穿网络法制备有机/无机杂化阴离子交换膜

以1,2-双(三乙氧基硅基)乙烷杂化改性的聚乙烯醇(PVA)为聚合物基底,以溴化聚乙烯亚胺(BPEI)为阳离子聚电解质,采用半互穿网络法制备新型阴离子交换膜.对含水率、离子交换容量及膜的形态进行了考察,并测试了膜的电导率及甲醇渗透率以研究其在直接甲醇燃料电池中的应用.测试结果表明,BPEI质量分数在40％时,PVA与BPEI互容性较好,无明显相分离现象产生.BPEI质量分数为20％的阴离子交换膜具有较好的综合性能,其在30℃下的电导率和甲醇渗透率分别为1.32×10-2 S/cm和7.88×10-7 cm-2·s-1.     

Effect of Jiaji electroacupuncture in transected rat spinal cord

We evaluated the effect of Jiaji electroacupuncture on cell proliferation and the expression of markers of endogenous neural stem cell activation after complete spinal cord transection. Female Wistar rats were assigned to 4 groups (n = 24 each): a sham-operated group, a control group, a Jiaji electroacupuncture group, and a Jiaji electroacupuncture preconditioning group. Motor function was significantly improved in the acupuncture groups compared to the control group at 7 and 14 d. Numbers of bromodeoxyuridine (BrdU)-, nestin-, and glial fibrillary acidic protein (GFAP)-positive cells were significantly greater in the acupuncture groups than in the controls at each time point. Expression of nestin and GFAP mRNA was significantly higher in the acupuncture groups than in the controls at each time point. Thus, Jiaji electroacupuncture and preconditioning may promote the proliferation of endogenous neural stem cells after spinal cord transection.     

Luminescent and photovoltaic properties of poly（9,9-dioctylfluorene- co-bithiophene） in organic electronic devices

We studied the luminescent and photovoltaic properties of poly(9,9-dioctylfluorene-co-bithiophene)(F8T2) based on ITO/PEDOT:PSS/F8T2/Bphen/LiF(0 or 1 nm)/Al and ITO/PEDOT:PSS/F8T2:PCBM/Bphen/Al.A stable and bright yellow emission was obtained from polymer F8T2,and the electroluminescence power reached 45 ?W at a 15 V driving voltage.Polymer F8T2 shows a broad absorption band from 400 to 500 nm,and has a shorter absorption edge at about 560 nm compared to that of the typical electron donor P3HT(650 nm).The photoluminescence quenching of F8T2 occurs with only a small fraction of blended PCBM due to the effective exciton dissociation at the interface between F8T2 and PCBM.Polymer solar cells(PSCs) using F8T2:PCBM as the active layer show a low power conversion efficiency(PCE) of 0.10% with an open circuit voltage(Voc) of 0.91 V and short circuit current density(Jsc) of 0.23 mA/cm2.The PSCs using F8T2:P3HT:PCBM as the active layer have a Voc of 0.85 V and Jsc of 3.02 mA/cm2,improving the PCE by about 0.90%.We attribute the improved cell performance to the higher number of photons harvested by P3HT molecules.     

High conversion efficiency of pristine TiO<Subscript>2</Subscript> nanotube arrays based dye-sensitized solar cells

We prepared highly-ordered titanium dioxide nanotube arrays (TNAs) by anodizing Ti foils in F-containing electrolytes.The crystalline nature and morphology of the TNAs were studied using X-ray diffraction patterns and scanning electron microscopy.We found the morphology of TNAs affects the light-to-electricity conversion efficiency (η) of dye-sensitized solar cells (DSSCs).The efficiency of DSSCs reached 5.95% under the condition of light illuminated from the counter electrode.The high efficiency of TNA-based DSSCs was attributed to the neat top surface of TNAs,which allows more dye molecule loading on the surface of the TiO 2 nanotubes,and fewer electron recombination centers and a low interface resistance of integrated TNAs.