Activation of proteinkinase ERK mediates induction of macrophage MMP-12 by OxLDL

The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of maerophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot analysis.Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner.OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12.These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway.     

非线性等重检错好码的存在性的进一步分析

(二元#),*+,’非线性等重码在数字通信中被广泛地用作检错码,人们一直研究一般#)"*+" 等重码成为检错好码的充要条件-首先讨论了二元#)"*+",’等重码成为检错好码的条件"得到了二元 最优等重码成为检错好码的两个充分必要条件"以及时"二元#)"*+",’最优等重码不 是检错好码的结论,同时,还讨论了,元非线性等重检错好码的存在性,并得出了一些新的结果-     

龙羊峡水电站空耗水量的考核研究

龙羊峡水电站在运行过程中,由于调度不当会造成机组开机台数过多而空耗水量。通过空耗水量计算方法的分析,结合龙羊峡水电站的实际情况,以及对空耗水量考核方案的选择,给出了考核标准及考核等级。     

树木成长过程的计算机仿真--人工生命的一个问题

本文从已有的原始模型开始,考虑了周围环境对树木生长的影响.即设定了一些随机变量加入到原始模型中,模仿环境中诸如阳光、空气和水分等因素对树木生长的影响.除此,本文同时展示了树木及地下树根部分协同生长的情况.在建模的同时,应用了Hansrudi Noser开发的Lworld.exe软件实现了整个模型的可视化.     

Real time monitoring of nucleic acids ligation based on molecular beacon

The replication, ligation and repair of nucleic acids are essential life processes that based the survival, pro-creation and evolution of creatures. These interactions involving proteins (enzymes) and nucleic acids have fas-cinated great interest of scientists[1]. The nucleic acids ligation has been revealed that it is catalyzed by ligase and co-enzyme (ATP or NAD)[2—4]. Along with in-depth re-search, the nucleic acids ligation becomes a significant and common tool employed in bioengineerin…     

The plasma membrane proton-translocating ATPase

Living cells require membranes and membrane transporters for the maintenance of life. After decades of biochemical scrutiny, the structures and molecular mechanisms by which membrane transporters catalyze transmembrane solute movements are beginning to be understood. The plasma membrane proton-translocating adenosine triphosphatase (ATPase) is an archetype of the P-type ATPase family of membrane transporters, which are important in a wide variety of cellular processes. The H+-ATPase has been crystallized and its structure determined to a resolution of 8 angstrom in the membrane plane. When considered together with the large body of biochemical information that has been accumulated for this transporter, and for enzymes in general, this new structural information is providing tantalizing insights regarding the molecular mechanism of active ion transport catalyzed by this enzyme.     

基于改进BP算法的刚框架连接损伤识别方法

运用权值、阈值同步学习BP算法(简称为改进BP算法)对钢框架连接损伤进行识别,并且选用由结构正则化的频率变化率和少数点的振型模态分量构成的组合参数作为网络输入参数.通过对一个4层钢框架数值模拟,比较了改进算法和传统算法测试结果,从而证明改进算法更加精确、优越.     

分组变权综合评估方法及应用

在分析变权综合决策方法的基础上,将变权分析理论引入多方案多评估指标的决策分析中,提出了一种分组变权综合评估方法,建立了分组变权综合评估的数学模型,并给出了一个应用案例。     

基于U-I模型的感应电机定子磁链估计的仿真研究

定子磁场定向的直接转矩控制逐渐被广泛应用于高性能异步电机调速系统。定子磁链估计精度是影响控制系统动态、稳态性能的主要因素。由于定子电阻参数变化及低通滤波器的影响，尤其低速运行时，传统定子磁链估计方法的估计精度及快速性往往很差。提出了一个全新的改进U-I模型定子磁链估计方案，不仅解决了定子电阻整定补偿问题。而且解决了低通滤波器截止频率优化补偿问题．在此基础上，建立直接转矩控制方案的仿真系统，仿真结果表明提出的方案能够使异步电机在低速到高速范围内都能得到令人满意的性能。     

不完全信息下的群体多属性决策方法

针对群体多属性决策问题,提出一种新的不完全信息下的群体多属性决策方法。该方法假设方案在主观评价属性下的评价值采用区间数的形式来表示专家评价值的不确定性和模糊性。针对每一主观评价属性设定一组与之对应的专家重要性权重值,反映决策者在不同评价属性中的重要性程度,同时考虑到专家评价值的相似度,将专家评价值进行集结得到专家群体关于方案集的决策矩阵。计算各方案与理想方案的相似度,并以此对方案进行优劣排序。通过实例说明所提方法的可行性。