一株内生真菌的原生质体制备及再生过程研究

通过对酶系组成和酶解条件的研究,得出了制备禾本科植物内生真菌Neotyphodiumsp.原生质体的最佳条件:鲜菌丝由10.3%蔗糖溶液预处理,酶系组成为1.5%崩溃酶、1.0%蜗牛酶、1.0%纤维素酶和2.0%溶菌酶,酶解温度32℃,pH 6.8,酶解5 h,原生质体得率达7.3×103个/mg鲜菌丝.另外,液体再生涂布平板法结合钙离子作用可使原生质体再生率达4.72%,为不含钙离子处理组的2.5倍.     

三种方法测定碱性磷酸酶对肝疾病的诊断价值

目的研究血清碱性磷酸酶检测临床肝疾病时的方法学,提高临床诊断价值。方法收集临床不同肝疾病病人的血清,分别用3种方法测定其碱性磷酸酶的活性,并与健康对照组比较作诊断性能分析。结果各肝疾病组血清碱性磷酸酶的活性与对照组相比有极显著差异（P〈0．001）;3种方法分别测定各组之闻的血清碱性磷酸酶活性无显著性差异（P〉0．05）。2．乙基氨基乙醇、2-氨基-2-甲基-1-丙醇、二乙醇胺3种缓冲液测定试剂盒方法测定的灵敏度分别为55．20％、47．96％、51．13％,特异度分别为100％、100％、93．75％。结论血清碱性磷酸酶可以作为各类肝病的临床诊断指标;对肝疾病血清碱性磷酸酶测定用2,乙基氨基乙醇缓冲液的试剂方法测定效果较好。     

Functions and pathologies of BiP and its interaction partners

The endoplasmic reticulum (ER) is involved in a variety of essential and interconnected processes in human cells, including protein biogenesis, signal transduction, and calcium homeostasis. The central player in all these processes is the ER-lumenal polypeptide chain binding protein BiP that acts as a molecular chaperone. BiP belongs to the heat shock protein 70 (Hsp70) family and crucially depends on a number of interaction partners, including co-chaperones, nucleotide exchange factors, and signaling molecules. In the course of the last five years, several diseases have been linked to BiP and its interaction partners, such as a group of infectious diseases that are caused by Shigella toxin producing E. coli. Furthermore, the inherited diseases Marinesco-Sj?gren syndrome, autosomal dominant polycystic liver disease, Wolcott-Rallison syndrome, and several cancer types can be considered BiP-related diseases. This review summarizes the physiological and pathophysiological characteristics of BiP and its interaction partners. Received 20 November 2008; received after revision 09 December 2008; accepted 12 December 2008     

修复周围神经损伤的静电自组装PDLLA/CS/CHS复合材料

利用静电自组装和真空冷冻干燥法制备用于修复周围神经损伤的聚乳酸/硫酸软骨素/壳聚糖复合材料.用全反射傅里叶红外光谱、扫描电子显微镜等进行表征,发现复合材料表面被均匀修饰,具有明显的多级结构,内层为致密结构,外层为疏松的海绵结构,孔径10~100μm.亲水性实验发现自组装复合材料的亲水性较纯聚乳酸有了较大的提高.降解实验发现:在0~2周,材料降解液pH值急剧下降,后期下降缓慢;降解过程中pH值都在7.0左右.MTT(四甲基偶氮唑盐)实验表明复合材料具有一定的促进雪旺细胞生长的作用.     

Role of serotonin in the hepato-gastroIntestinal tract: an old molecule for new perspectives

Beside its role as a neurotransmitter in the central nervous system, serotonin appears to be a central physiologic mediator of many gastrointestinal (GI) functions and a mediator of the brain-gut connection. By acting directly and via modulation of the enteric nervous system, serotonin has numerous effects on the GI tract. The main gut disturbances in which serotonin is involved are acute chemotherapy-induced nausea and vomiting, carcinoid syndrome and irritable bowel syndrome. Serotonin also has mitogenic properties. Platelet-derived serotonin is involved in liver regeneration after partial hepatectomy. In diseased liver, serotonin may play a crucial role in the progression of hepatic fibrosis and the pathogenesis of steatohepatitis. Better understanding of the role of the serotonin receptor subtypes and serotonin mechanisms of action in the liver and gut may open new therapeutic strategies in hepato-gastrointestinal diseases. Received 15 August 2007; received after revision 1 November 2007; accepted 5 November 2007     

酸解法对甘氨酸母液脱色后废活性炭的再生研究

在用海因法生产甘氨酸的过程中,为了精制甘氨酸,需要用大量的活性炭对甘氨酸母液进行脱色,而脱色后的活性炭就成了废活性炭.为了实现废活性炭的再利用,针对此废活性炭用酸解法对其进行再生.研究了再生酸种类、浓度、用量、再生温度和再生时间等对废活性炭再生效果的影响,确定了适宜的再生条件:硫酸质量分数为70％,硫酸溶液与废活性炭的...     

黄花蒿叶片植株再生体系的建立

以黄花蒿叶片为材料,设计离体培养不同阶段的培养基配方.通过对培养结果进行观察、统计分析,结果表明:MS 2,4-D(2.0mg/L)对叶片诱导愈伤组织效果最好;MS 6-BA(1.0mg/L)为丛生芽分化培养基的适宜配方;无菌苗在MS NAA(0.05mg/L)的培养基上生根效果良好.     

粪菌移植对肺炎克雷伯菌所致小鼠肝脓肿感染的抑制作用

探讨粪菌移植(FMT)对高致病性肺炎克雷伯菌(Klebsiella pneumoniae)所致小鼠肝脓肿(KLA)感染的抑制作用及相关机制.小鼠随机分为对照(Healthy)组、肺炎克雷伯菌(Kp)组和干预(FMT)组. Kp组小鼠经口灌胃10⁶ CFU K1血清型肺炎克雷伯菌;FMT组在给菌24 h后经口灌胃给予正常小鼠粪菌悬液(300 mg/mL,0.3 mL).结果表明,与Kp组相比,FMT处理明显降低了小鼠KLA感染率;16S rRNA高通量测序分析表明,FMT显著改变了小鼠肠道菌群组成,包括增加了Alloprevotella、norank＿f＿Muribaculaceae等益生菌的丰度;同时FMT处理明显降低了小鼠血清TNF-α和胃肠内防御素分子浓度,同时增加了肠黏蛋白2(MUC2)的表达.因此,肠道菌群和免疫防御因子可能一起参与了FMT对高致病性肺炎克雷伯菌所致小鼠KLA感染的抑制作用.     

电刺激小脑顶核对大鼠心肌梗死后心脏神经再生的影响

探讨预先电刺激小脑顶核(FNS)对大鼠心肌梗死(MI)后心脏神经再生的干预作用。将90只Wistar大鼠随机分为:①MI组,结扎左冠状动脉前降支(LAD);②预先电刺激小脑顶核后再予以LAD结扎组(FNS MI组);③毁损小脑顶核后电刺激该部位,再行LAD结扎组(FNL FNS MI组)。各组又分MI后1,7,21d3个时间点。另设假手术组(Sham组)。应用免疫组织化学方法显示心脏组织胆碱乙酰转移酶(CHAT)和酪氨酸羟化酶(TH)。检测各时间点梗死区与非梗死区TH、CHAT阳性神经纤维分布密度,MI组与Sham组相比显著减少(P<0.01);FNS MI组较MI组显著增多(P<0.05);FNL FNS MI组与MI组比较无显著性差异(P>0.05)。因此,电刺激小脑顶核可增加梗死区和非梗死区心肌组织TH、CHAT染色阳性神经纤维密度,促进MI后心脏神经纤维再生。     

恩替卡韦治疗失代偿期肝硬化疗效观察

目的观察恩替卡韦治疗失替偿期乙型肝炎肝硬化的疗效。方法对2006年3月至2007年6月18例来我院就诊乙型肝炎肝硬化失代偿期患者给予口服恩替卡韦治疗剂量0．5mg／次，1次／d，基本疗程1年。观察HBV-DNA定量，肝纤维化指标（HA、LN、POⅢ、Ⅳ—C），肝功能指标（ALT、AST、TBIL）变化情况。结果所有患者临床症状明显改善，肝功能指标、HBV—DNA定量、肝纤四项指标改善有非常显著差异（P〈0．01）。结论恩替卡韦治疗乙型肝炎失代偿期肝硬化可有效抑制病毒复制，改善肝功能，阻止肝纤维化进展，防止病情恶化。