Rapid induction of mRNAs forPC3 by partial hepatectomy and epidermal growth factor

The expression of immediate early gene plays a pivotal role in rat hepatocyte proliferation from G₀ to G₁ phases and the progression through G₁ phase of the cell cycle within several hours after 2/3 hepatectomy. We investigated the different gene expressions within 1 h after 2/3 hepatectomy by representational difference analysis. Sequence analysis indicated thatPC3 induced by NGF was a kind of immediate early gene and might be correlated with liver regeneration. Moreover, we found that 2/3 hepatectomy could induce the expressing ofPC3 mRNA by Northern blot with a peak 1–2 h after surgery. In primary cultures of rat hepatocytes, addition of EGF resulted in rapid and transient induction ofPC3 mRNA. It was first reported thatPC3 gene belongs to immediate early gene associated with liver regeneration.     

Mechanism for chronic pain generation

Neuropathic pain and the other abnormalities of sensation induced by axon injury or by peripheral nerve inflammation should result from functional compensations of the injured neurons during their regeneration. Ectopic distribution of proteins related to Na⁺, K⁺ and Ca²⁺ channels as well as of receptors on both membranes of injured axon and its cell body becomes a main pacemaker from which spontaneous ectopic afferent of primary sensatory neurons and crosstalk between neurons occur. Abnormal ectopic afferent activities lead to disorders of the sensation, such as hyperalgesia, allodynia, spontaneous pain and paraesthesia. Administration of some ion channel agents and/or α2-adrenergic blockers has shown efficiency in preventing neuropathic pain development and in relieving neuropathic pain.     

寄生虫与宿主的相互作用

通过对寄生虫与宿主的相互关系及影响因素进行研究,以有效地控制寄生虫病的发生.结果:不同种类的寄生虫具有不同的亲和性,可在机体的不同脏器或组织寄生.当有两种或两种以上的寄生虫感染时,它们通过相互协同或相互拮抗,共同参与寄生虫抗宿主反应,引起机械性损伤、营养不良、变态反应等.同时,机体对入侵的寄生虫产生一系列免疫反应,及时清除入侵的寄生虫,并具有抵抗再感染的能力,或形成免疫耐受.     

肥胖,高脂血症性脂肪性肝炎模型的建立

为建立大鼠肥胖、高脂血症性脂肪性肝炎（ＮＡＳＨ）的动物模型。取雄性ＳＤ大鼠１９只,随机人分为模型组（ｎ＝１０）和正常组（ｎ＝９）。模型组喂高脂饮食,即普通饲料基础上加１０％猪油,２％胆固醇。正常组喂普通饲料。观察一般情况血清肝功能、血脂,并行病理学检查。模型组体重、肝指数、血清总胆固醇、游高脂肪酸及转氨酶均显著高于正常组;病理学均出现弥漫性肝细胞脂肪变性,且１００％存在小叶内炎症,８０％出现汇管区     

逆行胰胆管注射牛磺胆酸钠诱导重症急性胰腺模型多器官损害观察

摘要 探索逆行胰胆管注射牛磺胆酸钠诱导大鼠重症急性胰腺炎模型及胰腺外多器官损害情况。采用12周龄SD大鼠35只,随机分为对照组和实验组,各3组,实验组逆行胰胆管注射牛磺胆酸钠诱导大鼠重症急性胰腺炎模型,对照组仅开腹后拨动十二指肠降部。观察造模后6小时、12小时胰腺和腺外器官病理和血清学指标变化、腹水及48小时内生存率。结果：SAP模型大鼠48小时内90%死亡,胰腺进行性水肿、坏死,大量腹水渗出,心、肺、肝、肾均有不同程度受损。SD大鼠逆行胰胆管注射牛磺胆酸钠诱导的动物模型,是研究重症急性胰腺炎胰腺坏事、水肿和多器官进损害的良好模型。     

冬奥会运动相关头部损伤与赛事场馆医疗保障

冬季奥林匹克运动头部损伤多见于高山滑雪、单板及自由式滑雪和冰球项目中,正确佩戴头盔、设置缓冲装置能够有效降低颅骨骨折发生率以及头部损伤严重程度.国内大型冰雪运动场地和赛事场馆周边的医疗基础相对薄弱,应汲取既往赛事和国外先进经验,结合实地情况从冬季运动的损伤发生特点出发,联合高水平医疗单位,提高诊断处置和转运效率,完善分...     

小儿肺炎并发心肌损伤心肌钙蛋白I的检测意义(附46例分析)

目的:探讨小儿肺炎并发心肌损伤心肌钙蛋白I的检测意义。方法:采用全自动化学免疫系统检测4 6例肺炎患儿心肌钙蛋白I(CTnI)、肌酸激酶同功酶(CKMB) ,并与2 6例正常小儿进行对照比较。结果:肺炎患儿与正常小儿CTnI、CKMB值比较均有非常显著性差异(P <0 0 1) ,且肺炎越重,越易造成心肌损害,二者值越高。两两比较,除重症肺炎组与普通肺炎组CKMB差异无显著性外(P >0 0 5 ) ,其余均有非常显著性差异(P <0 0 1)。结论:CTnI可作为监测小儿肺炎并发心肌损伤的重要标志物。     

研究汽车碰撞中头颈部动态响应的有限元模型的建立和验证

建立并验证了一个基于人体解剖学结构的头颈部三维有限元模型．该模型由颅骨、脑、颈椎骨、椎间盘、肌肉、韧带和小关节组成,总节点数为17758,单元数21803,模型生物材料特性分别用弹性和粘弹性模型描述．整个头颈部模型应用关国海军生物力学实验室前碰撞志愿者实验及查尔摩斯大学后碰撞滑车实验的数据进行了验证．采用该模型计算了头颈部的加速度、角速度等运动曲线及HIC值．验证结果显示该模型具有较好的生物逼真度,可用于研究在汽车碰撞事故中头颈部损伤生物力学问题和开发损伤防护装置．     

碱性成纤维细胞生长因子与脑缺血再灌注损伤

碱性成纤维细胞生长因子是由多种细胞分泌的具有多种生物学功能的细胞因子。它不仅能够促进周围神经的再生和延长外周神经元的存活,而且在动物脑缺血再灌注模型中具有重要的抗损伤作用,为临床上脑缺血再灌注损伤疾病的治疗带来了光明的前景。     

脂肪肝的发病机理和治理研究进展

综述了不同诱因所致脂肪肝的发病机制和治疗,为临床合理选择药物提供了方案。