鼻咽癌放疗后鼻咽局部复发的早期CT诊断

追踪观察了鼻咽癌放疗后的患者４３例，其中证实复发者１１例，鼻咽显示异常，但经追踪观察而否定复发者３２例，将复发与未复发病例的各种ＣＴ征象进行比较分析，提示在鼻咽癌放疗后局部复发的早期诊断中，“茎突内侧软组织增厚致密”为一重要征象，而“顶后壁增厚、侧壁增厚和咽隐窝变浅或消失”信     

An immunohistochemical study of the clearance of apoptotic cellular fragments

We investigated the distribution and fate of apoptotic bodies during human development and in the adult, using an antibody (M30) that recognizes a neo-epitope formed early in the apoptotic cascade by caspase cleavage of cytokeratin 18. In the fetus, we found extensive accumulation of M30-positive, non-phagocytosed fragments in the red pulp of the spleen, subcutaneous and submucosal vessels, the interstitium of the lung, and the glomerular mesangium of the kidneys. In the liver, M30-immunoreactive fragments were found inside macrophages in the sinusoids. The number of these fragments and the intensity of the immunostaining increased with the gestational age of the fetus. In the adult, M30-positive fragments were barely detectable in normal tissues. However, many pathological situations, including both chronic degenerative processes and metastatic cancer, were associated with accumulation of M30-positive fragments in the red pulp of the spleen. In the liver and kidney, no fragments could be detected. Remarkably, 13 of the 16 patients with metastasized cancer showed pronounced accumulation of M30-positive fragments containing hematoxylin-reactive material in the red pulp of the spleen. In the non-cancerous cases, such DNA-containing fragments were only seen in 9 of 94 cases. The results show that when apoptotic activity is high, as during development in the fetus or during metastasis and other pathological processes in the adult, the phagocytic clearance of apoptotic bodies can be overloaded. These apoptotic fragments then accumulate in the spleen. The visual detection of apoptotic fragments is concluded to reflect increased cell turnover. Received 1 July 2002; accepted 1 July 2002     

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Vectors pose most pivotal problem of gene therapy[1]. Because of the high transfection efficiency both in vitro and in vivo, the viral vector has been employed in 70% clinical trials of gene therapy (http://www.wiley.co.uk/ genmed/clinical). However, thei…     

平阳霉素碘油乳剂治疗肝海绵状血管瘤28例报告

目的 :探讨平阳霉素碘油乳剂栓塞治疗肝海绵状血管瘤 (CHL)的疗效及安全性。方法 :全组 2 8例 39个肝海绵状血管瘤 ,瘤体直径 3 0～ 2 8 0cm ,平均为 (6 3± 4 5 )cm。超选择插管至肿瘤供血动脉 ,注入平阳霉素碘油乳剂。其中 4例术后 3个月再次行栓塞治疗。结果 :术后病灶内碘油沉积良好 ,无严重并发症 ;2 8例 39个病灶术后 3个月、6个月复查结果显示 :瘤体缩小 >5 0 %者 18例 (6 4 3% ) ,缩小 30 %～ 5 0 %者 10例 (35 7% )。结论 :平阳霉素碘油乳剂栓塞治疗CHL安全有效 ,可作为CHL的首选治疗方法     

食管癌切除术后心律失常的分析治疗

对452例食管癌切除术后并发心律失常的69例病人的发病原因及治疗进行了分析.在 69例病人中,窦性心动过速 32例,占 46.4％;房性早搏 9例,占 13.1％;房室交界性早搏4例,占5.8％;室上性心动过速11例,占15.9％;室性早搏8例,占11.6％;房颤4例,占5.8％;室性心动过速1例.     

脂肪肝超声图像的定量分析

脂肪肝定量分析是对脂肪肝诊断的一种客观方法，可减少定性分析中人为因素所带来的偏差。对多例B超图像提取特征参数，并进行肝肾对比，从而在统计意义上分析正常肝与脂肪肝的参数差异性。     

大鼠肝细胞核仁超微结构与rDNA分布位点

以常规电镜及NAMA－Ur DNA特异染色技术对Wistar大鼠肝细胞核仁的超微结构和rRNA的分布进行了观察。常规电镜技术表明大鼠肝细胞核仁纤维中心（FC）是电子透明区，数量较多，形状不规则。密集纤维组分（DFC）是围绕FC的环状结构部分，电子密度很高。FC中的染色质存在着一个介于集缩和解集缩状态之间的变化过程。NAMA－Ur DNA特异染色技术表明核仁内rDNA呈分散性分布，主要分布于DFC中（包括FC）的边缘），同时观察到与核仁相随染色质相连的核仁内rDNA呈念珠状结构。     

用荷瘤裸鼠模型研制抗人肝癌单克隆抗体

1985年Watanabe等报道:用去胸腺载瘤小鼠的脾细胞与小鼠骨髓瘤细胞SP 2/0融合获得抗结肠癌单克隆抗体之后,利用荷瘤裸鼠模型成功研制抗人肿瘤单抗的报道已相继出现:但所获单抗多为IgM,IgG类的单抗得率不高,本工作采用多次手术大部切除瘤块的方法延长荷瘤裸鼠的成活期,获得一株抗人肝癌IgG类单抗、其研制过程和结果报道如下。     

Ventral neural tube cells differentiate into hepatocytes in the chick embryo

A population of ventral neural tube cells has recently been shown to migrate out of the hind brain neural tube via the vagus nerve and contribute to the developing gastrointestinal tract. Since liver is also innervated by the vagus nerve, we sought to determine if these cells also migrate into the liver. Ventral neural tube cells in the caudal hindbrain of chick embryos were tagged with a replication-deficient retroviral vector containing the LacZ gene on embryonic day 2. Embryos were processed for detection of labeled cells on embryonic day 5 and 11. Labeled cells were seen in the liver on both days and identified as hepatocytes. Previously, it was believed that all hepatocytes develop from the gut endoderm. Results of the present study show an additional source for the formation of liver cells. Received 25 August 1998; received after revision 5 November 1998; accepted 5 November 1998