Proteomic analysis of low-abundant integral plasma membrane proteins based on gels

To characterize low-copy integral membrane proteins and offer some methods for human liver proteome projects, we fractionated highly purified rat liver plasma membrane (PM). PM was purified through two sucrose density gradient centrifugations, and treated with 0.1 M Na₂CO₃, chloroform/methanol and Triton X-100. Proteins were separated by electrophoresis and submitted to mass spectrometry analysis. Four hundred and fiftyseven non-redundant membrane proteins were identified, of which 23% (105) were integral membrane proteins with one or more transmembrane domains. One hundred and fifty-three (33.5%) had no location annotation and 68 were unknown-function proteins. The proteins from different fractions were complementory. A database search for all identified proteins revealed that 53 proteins were involved in the cell communication pathway. More interestingly, more than 50% of the proteins had a protein abundance index concentration of less than 0.1 mol/l, and 12% proteins a concentration 100 times less than that of arginase 1 and actin. Received 15 March 2006; received after revision 17 May 2006; accepted 10 June 2006 L.-J. Zhang and X.-e Wang are contributed equally to this work.     

Hitting malaria before it hurts: attenuated <Emphasis>Plasmodium</Emphasis> liver stages

Continuous natural exposure to Plasmodium transmission by infectious Anopheles mosquitoes leads to a gradual acquisition of immunological competence against malaria. The partial immunity, observed in adolescents and adults living in endemic areas, reduces morbidity and mortality without preventing parasite infection. In experimental animal models, long-lasting sterilizing immunity can be achieved with genetically attenuated Plasmodium liver stages. Can these findings be translated to accomplish sterile protection against natural malaria transmission in the high-risk group, young infants in sub-Saharan Africa?     

基于二维规则网格的SIRS病毒传播模型

提出了一个基于二维规则网格的SIRS疾病传播模型,在模型中,研究了群体密度d, 传播效率λ及个体的游动对疾病传播的影响。理论分析和仿真模拟表明该疾病传播模型存在一个临界值(λd)c,只有当群体传播效率和群体密度的乘积λd大于(λd)c时,疾病才能在群体中持续稳定地传播。 另外,研究还发现当群体密度不太大的时候个体的游动更有利于疾病的传播。 根据这些研究结果最后给出了相应的疾病预防和控制措施。     

高频电流模四象限CMOS模拟乘法器

本文提出了一种新型四象限CMOS模拟乘法器,其核心组成为工作于三极管区的NMOS晶体管和高频CCII(第二代电流传输器)电路.基于2μmP阱CMOS工艺参数的模拟结果显示,当电源电压为±5V,在±4V的输入范围内,其非线性误差小于2.8‰,运算误差小于3.3‰.X输入端的-3dB带宽为22MHz,Y输入端的-3dB带宽为82MHz.     

浅议营造工程量清单计价的环境

文章从提高计价人员素质、强化设计及保证设计质量、规范工程变更程序三方面进行分析,阐述了营造工程量清单计价环境的重要性。     

提高学生生产实习教学质量的探讨

对如何进一步提高生产实习教学质量及改进生产实习教学方法进行了探讨 ,提出充实生产实习教学内容及加强实习管理的措施 ,采取集中—分散生产实习相结合的教学模式 ,并通过实践取得了较好效果     

发电机原动系统仿真器程序设计

研制了一种新型的基于80C196KC单片机的原动机仿真系统的软件．对系统中的调速器,利用灵活的单片机的软件去实现其功能,不仅使硬件线路大为简化,并且克服了模拟电路结构复杂、电子元器件容易老化、易受环境温度影响和抗干扰能力差等缺点,提高了整个系统的控制精度、可靠性和稳定性．此外还设置了液晶显示器和人机通讯接口,可将原动机仿真系统的运行参数以数字信号的形式传输到监控系统或者PC机,操作直观、便于监视．微机软件编写采用了模块化,使软件的编写更方便、简单,软件已完全通过调试,达到了预期的效果。     

大鼠肝再生中Cyclin D1、PCNA的动态变化

采用蛋白质印迹技术分析了肝切除不同恢复时期cyclin D1 和PCNA的含量变化,结果显示两者表达趋势基本一致,呈显著正相关(r=0.619**,P<0.01),提示cyclin D1 和PCNA在肝再生中细胞周期的 G1/S期转换和S 期DNA合成中发挥关键作用.     

在系统可编程模拟电路ispPAC及其应用

介绍了在系统可编程模拟电路ispPAC的结构及开发过程,并给出了利用ispPAC10来设计双二阶低通滤波器的实例。     

ISD2500系列语音芯片及其在微机系统中的应用

应用直接模拟存储技术,在一个集成电路芯片内,直接完成语音模拟信号的录入、存储和调出,这是当前国际电子领域,在固态语音集成电路方面的最新成果,以ISD2500系列语音芯片为例,介绍了该种语音芯片的特点、使用方法及在单片机应用系统中的应用。