翻译控制肿瘤蛋白(TCTP)研究进展

翻译控制肿瘤蛋白（The translationally controlled tumor protein,TCTP）最初发现于肿瘤组织,后来又从其他组织中相继分离出来。TCTP是一类广泛存在于动物、植物及酵母中的、在序列上高度保守且具有很高表达量的蛋白家族。最初认为TCTP是一类生长相关蛋白,近年来研究表明,代TP具有多种生物学功能。     

用AFM观察小白鼠心肌核DNA片段的基因体外转录和垃圾DNA的相互作用

用AFM直接观察、体外转录等实验技术组合,发现小白鼠(Balb/C)心肌体外转录状态的核DNA片段上的各种基因,处于垃圾DNA的特定的“转录平台”上。“转录平台”上的各种核活性基因的两端的调控序列,分别与特定开关蛋白质复合体结合(即可解离的开关蛋白质),中间的编码序列分别以非共价键特异结合可完全解离的转录活性因子等多种蛋白质;这些与核基因转录相关的蛋白质均由垃圾DNA的专一性蛋白质通路分别进行特异性正负反馈调控。     

人性化管理在图书馆规章制度中的运用探析

从人性化管理的内涵出发,探讨了人性化管理在图书馆规章制度的运用,指出人性化管理是图书馆管理的主流及发展方向。     

基于径向基函数网络或模糊系统的非线性连续时间系统的自适应调节

对于一类连续时间的非线性动态系统x=f(x)+Bu+d,当系统中的非线性函数f(x)满足有界或线性增长条件(具有未知的增长系数)时,首先证明了f(x)中的x落入一紧集中,然后根据径向基函数网络或模糊系统的逼近性质,给出了两种自适应调节器的设计方法.利用李亚普诺夫稳定性理论,证明了控制算法是全局稳定的,闭环系统的状态是一致最终有界的,且控制律是稳定的.     

The regulation of ion channels and transporters by glycolytically derived ATP

Glycolysis is an evolutionary conserved metabolic pathway that provides small amounts of energy in the form of ATP when compared to other pathways such as oxidative phosphorylation or fatty acid oxidation. The ATP levels inside metabolically active cells are not constant and the local ATP level will depend on the site of production as well as the respective rates of ATP production, diffusion and consumption. Membrane ion transporters (pumps, exchangers and channels) are located at sites distal to the major sources of ATP formation (the mitochondria). We review evidence that the glycolytic complex is associated with membranes; both at the plasmalemma and with membranes of the endo/sarcoplasmic reticular network. We examine the evidence for the concept that many of the ion transporters are regulated preferentially by the glycolytic process. These include the Na⁺/K⁺-ATPase, the H⁺-ATPase, various types of Ca²⁺-ATPases, the Na⁺/H⁺ exchanger, the ATP-sensitive K⁺ channel, cation channels, Na⁺ channels, Ca²⁺ channels and other channels involved in intracellular Ca²⁺ homeostasis. Regulation of these pumps, exchangers and ion channels by the glycolytic process has important consequences in a variety of physiological and pathophysiological processes, and a better understanding of this mode of regulation may have important consequences for developing future strategies in combating disease and developing novel therapeutic approaches. Received 20 July 2007; received after revision 30 July 2007; accepted 17 August 2007     

Targeted inhibition of Livin resensitizes renal cancer cells towards apoptosis

Cancer cells are typically characterized by apoptosis deficiency. In order to investigate a possible role for the anti-apoptotic livin gene in renal cell cancer (RCC), we analyzed its expression in tumor tissue samples and in RCC-derived cell lines. In addition, we studied the contribution of livin to the apoptotic resistance of RCC cells by RNA interference (RNAi). Livin gene expression was detected in a significant portion of RCC tumor tissue specimens (13/14, 92.9%) and tumor-derived cell lines (12/15, 80.0%). Moreover, targeted inhibition of livin by RNAi markedly sensitized RCC cells towards proapoptotic stimuli, such as UV irradiation or the chemotherapeutic drugs etoposide, 5-fluorouracil, and vinblastine. These effects were specific for livin expressing tumor cells. We conclude that livin can contribute significantly to the apoptosis resistance of RCC cells. Targeted inhibition of livin could represent a novel therapeutic strategy to increase the sensitivity of renal cancers towards pro-apoptotic agents. Received 30 November 2006; received after revision 22 February 2007; accepted 20 March 2007     

农田灌溉水循环利用系统构建方法

介绍了将农田中坑塘湿地改建用于储存降雨径流和农田灌溉排水进行回用的水循环系统构建方法,在提出水循环调蓄湿地体积估算方法的基础上,构建了调蓄湿地水循环利用系统,分析了高效净污载体、微生物筛选与附着、水生植物群落配置和水动力微循环交换系统等水质净化技术,指出区域降水量和降雨历时是影响水循环调蓄湿地体积和循环水利用量的重要因素。对上海崇明水稻田循环调蓄湿地系统进行了设计与构建,循环调蓄湿地运行后共节约灌溉水量6 239.9～9 359.9 m³。     

市场机制和政府调控下的产学研合作创新网络演化博弈仿真——以新能源汽车产业为例

以新能源汽车产学研合作创新网络为实证分析对象,运用网络演化博弈理论和仿真分析方法,剖析在市场机制和政府调控下,产学研合作创新网络中创新主体合作行为与网络结构在互动过程下的演化规律。研究结果表明：市场机制和政府调控对于创新主体维护合作行为和网络集聚都具有促进作用;创新主体合作行为与网络结构之间具有协同互动关系,并且市场机制和政府调控的作用越大,两者间协同互动作用越明显。在市场机制下,市场机制作用的增强会降低网络演化的速度;只有当市场机制作用超过一定阈值时,维护合作行为才会在创新主体间涌现出来。在政府调控下,创新主体对于政府调控较为敏感;当政府调控力度处于阈值水平时,不利于创新主体间合作关系的维持。     

水肥高效利用灌排系统调控面临的问题研究

通过调查我国水肥利用的现状,指出我国农业水利中干旱缺水、非点源污染、水资源利用率低下和灌排系统不配套等问题,提出灌区水肥高效利用需深入研究的热点问题,对探讨水肥高效利用和灌排系统管理理论,指导我国灌排系统精量调控,以最少的水肥投入量获取最佳的灌区产量和经济及生态环境效益,具有重要意义.