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931.
932.
Protein-protein recognition is an important step in biological processes, which still largely remains elusive. The inter-residue contact potential, CPij, describes the propensity of contact between two types of residue. In this study, several different CPij variants were examined with the objective of discriminating the binding potential of surface pairs. Using solvent mediated inter-molecule contact potential (SM-IMCPij), an evaluation model was deduced and tested. Using the evaluation model it was found that the SM-IMCPij gives a better performance than either residue mediated IMCPij(RM-IMCPij) or folding-residue contact potential (FCPij). The results suggest that the evaluation model provides a fast, effective, and discriminative method for the evaluation of proposed binding interfaces. 相似文献
933.
瓶插杜鹃花的保鲜研究 总被引:1,自引:0,他引:1
汪劲松 《湖北师范学院学报(自然科学版)》2002,22(1):76-77
游离脯氨酸含量的升高和可溶性蛋白的下降均标志着切花的衰老,切花杜鹃950mg/L6-BA 200mg/L8-HQS 100mg/LCA 2%蔗糖)处理后,可以明显降低游离脯氨酸上升的幅度和延缓可溶性蛋白降解的速度,从而延缓切花衰老。 相似文献
934.
Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes 总被引:3,自引:0,他引:3
Rodriguez AM Monjo M Roca P Palou A 《Cellular and molecular life sciences : CMLS》2002,59(10):1714-1723
The brown adipose tissue (BAT) thermogenic response to diet-induced obesity and cold has been found to be gender dependent.
In the present work, we aimed to investigate the effects of the main physiological male and female sex hormones, i.e. testosterone,
progesterone and 17-β-estradiol, on the expression of uncoupling protein 1 (UCP1) – the main mediator of BAT thermogenesis
– and on UCP2 and lipid accumulation in rodent brown adipocytes differentiated in culture. Testosterone-treated cells showed
fewer and smaller lipid droplets than control cells and a dose-dependent inhibition of UCP1 mRNA expression, under adrenergic
stimulation by norepinephrine (NE). These effects were reverted by the androgen receptor antagonist flutamide, suggesting
they are dependent, at least in part, on the androgen receptor. Progesterone- and 17-β-estradiol-treated cells showed more
and larger lipid droplets and progesterone stimulated NE-induced UCP1 mRNA expression at the lower concentration tested, but
not at higher concentrations, suggesting that for brown adipocytes, this hormone is dose dependent. 17-β-Estradiol did not
have any remarkable effect either on UCP1 or UCP2 mRNA expression. Interestingly, the specific progesterone receptor antagonist
RU486 induced UCP1 and UCP2 mRNAs, including UCP1 mRNA expression in non-NE-treated brown adipocytes, suggesting a profound
effect of this anti-progestagen on brown adipocyte thermogenic capacity. Thus, are conclude that testosterone, 17-β-estradiol,
progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid
accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response.
Received 24 June 2002; received after revision 20 August 2002; accepted 26 August 2002
RID="*"
ID="*"Corresponding author. 相似文献
935.
Sauvage E Kerff F Fonzé E Herman R Schoot B Marquette JP Taburet Y Prevost D Dumas J Leonard G Stefanic P Coyette J Charlier P 《Cellular and molecular life sciences : CMLS》2002,59(7):1223-1232
Penicillin-binding proteins (PBPs) are membrane proteins involved in the final stages of peptidoglycan synthesis and represent the targets of beta-lactam antibiotics. Enterococci are naturally resistant to these antibiotics because they produce a PBP, named PBP5fm in Enterococcus faecium, with low-level affinity for beta-lactams. We report here the crystal structure of the acyl-enzyme complex of PBP5fm with benzylpenicillin at a resolution of 2.4 A. A characteristic of the active site, which distinguishes PBP5fm from other PBPs of known structure, is the topology of the loop 451-465 defining the left edge of the cavity. The residue Arg464, involved in a salt bridge with the residue Asp481, confers a greater rigidity to the PBP5fm active site. In addition, the presence of the Val465 residue, which points into the active site, reducing its accessibility, could account for the low affinity of PBP5fm for beta-lactam. This loop is common to PBPs of low affinity, such as PBP2a from Staphylococcus aureus and PBP3 from Bacillus subtilis. Moreover, the insertion of a serine after residue 466 in the most resistant strains underlines even more the determining role of this loop in the recognition of the substrates. 相似文献
936.
The intracellular signaling pathways mediating the nuclear exclusion of the androgen receptor (AR) by melatonin were evaluated
in PC3 cells stably transfected with the AR. The melatonin-induced nuclear exclusion of the AR by melatonin (100 nM, 3 h)
was blocked by LY 83583 (an inhibitor of guanylyl cyclases). 8-Bromo-cGMP (a cell-permeable cGMP analog), mimicked the effect
of melatonin, as did ionomycin (a calcium ionophore) and PMA [an activator of protein kinase C (PKC)], and their effects were
blocked by GF-109203X (a selective PKC inhibitor). BAPTA (an intracellular calcium chelator) blocked the effects of melatonin
and 8-bromo-cGMP but not of PMA. Inhibition or activation of the protein kinase A pathway did not affect basal or melatonin-mediated
AR localization. We conclude that the melatonin-mediated rise in cGMP elicits AR nuclear exclusion via a pathway involving
increased intracellular calcium and PKC activation. These results define a novel signaling pathway that regulates AR localization
and androgen responses in target cells.
Received 31 July 2001; received after revision 18 September 2001; accepted 30 October 2001 相似文献
937.
D.T.A. Lamport 《Cellular and molecular life sciences : CMLS》2001,58(10):1363-1385
This review of the living cell wall [1] and its protein components is in two parts. The first is anecdotal. A personal account
spanning over 40 years research may perhaps be an antidote to one stereotypical view of scientists as detached and humorless.
The second part deals with the meaning of function, particularly as it applies to hydroxyproline-rich glycoproteins. Function
is a difficult word to define objectively. However, with help from such luminaries as Humpty Dumpty: "A word means what I
want it to mean, neither more nor less," and Wittgenstein: "Giving examples of usage ... is the only way to talk about meaning,"
it is possible to construct a ziggurat representing increasingly complex levels of organization from molecular structure to
ecology. Forty years ago I suggested that hydroxyproline-rich structural proteins played a key role in cell wall functioning.
But because the bulk of the wall is carbohydrate, there has been an understandable resistance to paradigm change. Expansins,
paradoxically, contribute greatly to this resistance because their modus operandi as cell-wall-loosening proteins is based
on the idea that they break hydrogen bonds between polysaccharide chains allowing slippage. However, this view is not consistent
with the recent discovery [Grobe et al. (1999) Eur. J. Biochem 263: 33-40] that β-expansins may be proteases, as it implies that the extensin network is not a straightjacket but a substrate for expansin
in muro. Such a direct role for extensins in both negative and positive regulation of cell expansion and elongation may constitute
a major morphogenetic mechanism operating at all levels of plant growth and development. 相似文献
938.
939.
Mechanisms of self-incompatibility in flowering plants 总被引:14,自引:0,他引:14
Self-incompatibility is a widespread mechanism in flowering plants that prevents inbreeding and promotes outcrossing. The
self-incompatibility response is genetically controlled by one or more multi-allelic loci, and relies on a series of complex
cellular interactions between the self-incompatible pollen and pistil. Although self-incompatibility functions ultimately
to prevent self-fertilization, flowering plants have evolved several unique mechanisms for rejecting the self-incompatible
pollen. The self-incompatibility system in the Solanaceae makes use of a multi-allelic RNase in the pistil to block incompatible pollen tube growth. In contrast, the Papaveraceae system appears to have complex cellular responses such as calcium fluxes, actin rearrangements, and programmed cell death
occurring in the incompatible pollen tube. Finally, the Brassicaceae system has a receptor kinase signalling pathway activated in the pistil leading to pollen rejection. This review highlights
the recent advances made towards understanding the cellular mechanisms involved in these self-incompatibility systems and
discusses the striking differences between these systems.
Received 10 May 2001; received after revision 20 June 2001; accepted 20 June 2001 相似文献
940.