优先队列在物流配送管理中的应用研究

针对物流企业在配送管理业务流程中的要求与特点，提出了采用优先级队列来实现分拣配货的动态调度观点，并对涉及动态调度过程中的相关数据结构与算法进行了设计，采用此技术可以提高企业的经济与社会效益．     

高师体育教育专业毕业生就业期望的调查研究

运用问卷调查法、文献法、访谈法、数理统计法等,对福建省高师体育教育专业毕业生的就业期望进行调查,旨在分析影响体育教育专业毕业生就业中存在的问题,并提出相应的对策与建议：加强对大学生的就业指导;政府必要的就业政策引导发挥宏观调控作用;充分调动学生自主择业的能力;提高毕业生的综合素质.     

跨世纪的高校图书馆馆员素质建设思考

分析了新时期高校图书馆馆员素质现状和图书情报服务工作的特点，论述了培养，提高自身综合素质是做好服务工作的基础和关键，开展继续教育则是提高素质的重要途径。     

基于JIT的多目标并行多机调度问题的混合遗传算法

针对一类极小化 makespan和延迟区间的并行多机零件排序问题 ,设计了一个混合遗传算法 .该算法的特点是充分利用求解单机问题的最优有效算法 ,设计了一种简单的编码方案 ,简化了算法的实现难度 ,提高了算法的执行效率 .数值仿真实验表明 ,该算法具有收敛速度快、优化效果好等特点 ,且适合于求解大规模问题 .     

岗位知识含量指标体系的构建及实证

从工作分析角度入手,利用以PAQ为基础改造的《企业员工生产率调查问卷》,对不同类型的单位及工作岗位上进行知识含量调查,从中归纳出衡量一般岗位知识含量的指标体系,并将岗位按知识含量高低进行分级,初步验证了知识含量不同的岗位之间不同指标维度上的区别,从而为深入研究知识工作生产率问题奠定一个基础.     

高校学生工作创新机制探新

高等学校的任务是培养具有创新精神和实践能力的高级专门人才，发展科学技术文化，促进社会主义现代化建设。如何培养具有上述特点的人才，是高校大学生管理思路与模式改革的出发点和归宿。改革大学生管理思路与模式是大学生素质培养的关键。     

浅论聋人高等教育的专业建设

盲人,不仅是社会中最困难的群体,也是残疾人中的困难群体.多少年来,他们的生活水平一直处在社会的下层.近年来,随着特殊教育的发展,特别是特殊中、高等教育的发展,给盲人的命运带来了极大的转机.但同样接受过高等教育的聋人,他们目前的出路就远比不上同学历的盲人.由此,我们应该以思考聋人高等教育的专业建设问题来解决聋人的出路问题.     

带二维装箱约束的需求可拆分车辆路径问题模型及算法研究

车辆配送计划时通常会考虑货物易损、易碎的可能性,以及如何充分利用有限的运输资源对货物进行配送以满足顾客的需求。在车辆有限、货物易损坏、顾客需求可进行拆分等环境下,实现车辆配送总路径最短,即考虑二维装箱约束的客户需求可拆分的车辆路径问题。对问题进行详细定义,建立了2L-SDVRP模型。将遗传算法与BLF算法的结合求解模型;并用数值案例验证算法有效性。     

Preparation and cellular uptake of PLGA particles loaded with lamivudine

Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles loaded with lamivudine and coated with bovine serum albumin (BSA) were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, concentration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtaining PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of ~35%. The particles were observed by scanning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.     

Enhancement of the efficiency of PEI/liposome transfection by magnetofectins formed via electrostatic self-assembly

One of the major challenges for successful gene therapy is improving the transfection efficiency of non-viral vectors. Magnetic nanoparticles (MNPs) have been developed as enhancers of non-viral vehicles. We prepared MNPs and modified them with polyethyleneimine (PEI), citric acid (CA) or carboxylmethyl-dextran (CMD). Both positively charged MNPs (MNPs@PEI) and negatively charged MNPs (MNPs@CA, MNPs@CMD) could spontaneously form transfection complexes (magnetofectins) with plasmid DNA and PEI/liposome via electrostatic self-assembly. Our results showed as-prepared magnetofectins apparently enhanced PEI/liposome transfection efficiency and/or gene expression level into COS-7 cells with reduced transfection time from 4 h to 15 min under a magnetic field in vitro. Meanwhile, the effect of magnetofection was cell line-dependant. These results suggest that charged MNPs could improve transfection efficiency for non-viral vectors by simply mixing with them and by exerting a magnetic force. Thus such MNPs provide a convenient platform for further applications of gene delivery.