Expression of recombinant human lysozyme in the milk of transgenic mice

Human lysozyme is a 130-aa (amino acid) alkaline polypeptide, and has both anti-bacterial and anti-viral properties which make it an important component of human natural immunity system. As a first step toward the ultimate goal of improving the anti-bacterial properties of bovine and ovine milk, a transgenic mouse that contains the genomic DNA sequence of the human lysozme gene has been generated for the first time. From 83 mice generated by microinjection, a total of 6 positive transgenic mice were identified by PCR and Southern blot. F1 mice positive for transgene in lines were also detected by PCR. This shows that transgene could be transmitted from founder transgenic mice to their offspring. Recombinant human lysozyme (rHlys) was found in the whey of 3 female positive transgenic mice by Western blot. The highest concentration of rHlys for transgenic mice was 0.2mg/mL. The antibacterial activity of the whey for transgenic mice was highly enhanced up to 0.4 times as much as that of human, while that of non-transgenic mouse was very low. Although the lysozyme activity of transgenic mice is still lower than that of human, the rHlys exhibits the same specific activity as that of human lysozyme. It provides a strong basis for further studies into the possible application of rHlys express in mammary gland.     

矿化垃圾反应床处理渗滤液的微生物学特性

研究了矿化垃圾生物反应床处理渗滤液时,不同床层高度、气温状况和水力负荷下矿化垃圾微生物学特性的变化规律.结果表明：床体浅层（0-20 cm）通透性好,细菌总数是床体厌氧区的数十倍,生理生化过程强烈,酶活性高,是污染物降解的最佳区域;气温的日变化对细菌总数影响不大,但在25-30℃时有利于维持微生物数量及其重要酶的活性;反应床对水力负荷有一定的耐冲击能力,但当矿化垃圾与渗滤液的体积比小于20（水力负荷超过16 cm.d^-1）时,床层微生物及酶面临胁迫环境,出水水质急剧变差.     

等离子束表面冶金涂层技术研究与应用

对等离子束表面冶金涂层技术与其他相近技术进行了比较。等离子束表面冶金涂层技术是一种快速非平衡冶金反应过程,类似粉末冶金,原则上可不受组成物的相溶性、熔点、密度等性质的限制,因此不需要预制合金雾化粉,可利用任意粉末的任意配比,获得通常冶金方法不能得到的(组织)物相。等离子束表面冶金涂层技术效率高,操作简便,成本低,应用前景广阔。     

自振阀式低压脉冲射流调制器振动特性仿真研究

基于脉动低压波克服井底压差提高钻速的机理，并根据自振阀式低压脉冲射流调制器的工作原理，利用Matlab环境下的Simulink系统建立了调制器液压振动力学的计算机仿真模型，对调制器的阀系运动进行了运动仿真。结果显示，调制器上阀和下阀协调振动具有可行性，调制器振动频率随钻头压降的增大线性增加，随钻井液流量、下阀质量、上阀行程的增加而减小。     

Hurst’s analysis to detect fluctuation of the jet in a two-dimensional fluidized bed

The experimental study of vertical jet in a two-dimensional fluidized bed is presented. The time series of vertical jet penetration depth are acquired by image processing. The fluctuation of jet which is studied by means of Hurst’s analysis becomes weaker with increase in jet velocity, fluidization number, particle diameter and static bed height. They are found that the signal is persistent and the fractal dimensional of vertical jet is between 1. 19 and 1. 64. Foundation item: Supported by Doctoral Foundation of China Biography: Zhou Ya-ming (1971-), male, born in 1971, Ph. D Candidate, majored in basic theory of two-phase fluid and fluidized bed.     

紊动射流对叶栅内气相流场的影响

从空气动力学的角度提出了“气垫防磨”理论，并以气垫叶栅作为研究对象，在不同的主流与射流速度比、不同的缝隙数和缝隙宽度等条件下，利用IFA300型热膜风速仪系统对叶片表面附近的流场进行了测量，得出了各工况下的气垫厚度，结果表明，增加射流速度、缝隙宽度和缝隙数量，气垫厚度也相应增加，并且气垫的厚度主要由叶片前半部分的射流决定，在理论上论证了垫防磨的可行性。     

湍流浸没射流反应区结构的数值计算

介绍HC1气体以湍流浸没喷入NH_3水溶液容器内而发生化学反应的反应区结构的数值计算。通过化学平衡的计算,求得以混合分数为基础的状态关系;采用局部均相流动模型(LHF)、κ-ε-g湍流模型、截尾正态分布模型和法夫雷(Favre)均化的榆运方程组,得到反应区内空隙率、温度、气体穿透距离和各组分的浓度等参数值。     

低弹网络丝在喷嘴丝道内运动规律探讨

本论文借助于实验手段揭示网络加工过程中,低弹丝在喷嘴丝道内受气流作用时的运动规律和气流在喷嘴丝道内的流动规律,借以揭示网络丝的网络结形成机理,为合理设计网络喷嘴提供依据。     

金属电解加工表面直纹成因的研究

对出现在热轧钢制件表面的电液压加工“直纹”疵病进行了实验分析，找出了其与钢中残余元素磷、铜含量之间的内在联系．     

分散式污水处理综述

阐述了分散式污水处理的概念,介绍了分散污水处理系统的类型和技术,并对污水集中处理和分散处理进行了比较。