基于嵌入式ARM9-Linux网络视频监控系统的设计与实现

基于ARM9和嵌入式Linux操作系统,对Linux内核gspca驱动程序进行了移植,配置了支持mjpg - streamer和uvcvideo的基础连接库,编译、移植mjpg - streamer软件包,实现了一个易搭建,易配置,通用性好,能让客户机通过浏览器实时监控远程目标的网络视频监控系统,可以用在无人值守的仓库、大规模的森林以及高速的交通工具.     

基于ARM9的机顶盒方案设计

将菲利浦公司的模拟电视信号采集处理芯片TDA8275、TDA8290和该公司的解码芯片SAA7133,同三星公司的具有PCI接口的ARM9核S3C2510A相结合,设计出具有存储电视节目和上网功能的机顶盒,具有一定的应用价值和市场前景.     

扩增引进限制性酶切位点技术检测CYP2C9基因的多态性

建立了一种简便、快速、准确的检测CYP2C9基因Arg144Cys位点和Ile359Leu位点多态性的新方法.该方法采用与模板具有1个碱基错配的引物通过PCR在扩增产物中引进限制性酶切位点,即扩增引进限制性酶切位点(amp lification-created restriction sites,ACRS),其中通过上游引物在扩增Arg144Cys位点的片断中引入AvaⅡ切点,使得野生型基因CYP2C9*1和突变型基因CYP2C9*2的PCR产物中分别含有2个和1个AvaⅡ切点;通过下游引物在扩增Ile359Leu位点的片断中引入MvaⅠ切点,使得野生型基因CYP2C9*1和突变型基因CYP2C9*3的PCR产物中分别含有1个和2个MvaⅠ切点.将来自Arg144Cys位点和Ile359Leu位点的PCR产物各取一份测序,表明错配碱基成功引入PCR产物中.将包含Arg144Cys位点的PCR产物用AvaⅡ消化,将包含Ile359Leu位点的PCR产物用MvaⅠ消化,然后通过聚丙烯酰胺凝胶电泳-银染进行基因分型.由于野生型基因和突变型基因的PCR产物中都至少含有1个酶切位点,能完全避免因限制性核酸内切酶失活或酶切不完全而导致的基因分型错误.     

高能球磨制备0.3BiFeO3-0.7SrBi2 Nb2O9陶瓷及其介电性能研究

采用高能球磨制备0.3 BiFeO3-0.7SrBi2Nb2O9陶瓷.利用XRD和SEM分析球磨时间对粉体物相结构及微观形貌的影响.结果表明:随球磨时间的增加,粉体结晶度提高,颗粒不断细化,但在22.62°处有杂峰出现,经700℃退火杂峰消失.高能球磨制备的陶瓷的介电常数较传统固相反应制备的陶瓷提高30左右.     

The protease-activated receptor-3 (PAR-3) can signal autonomously to induce interleukin-8 release

Protease-activated receptors (PARs) play a clear role in the burst of inflammatory reactions and immune responses. However, for PAR-3, the most elusive member of the PAR family, the functional role is still largely unclear. It has been claimed that PAR-3 does not signal autonomously, although the wide expression of human PAR-3 indicates its important physiological roles. We demonstrate that in HEK-293 cells, stably transfected with human PAR-3, thrombin induced calcium signaling, IL-8 gene expression and IL-8 release. We confirmed this finding using human lung epithelial and human astrocytoma cells that express endogenous PAR-3. Moreover, thrombin exposure of HEK-293 cells resulted in ERK1/2 activation coinciding with IL-8 release. The effects of thrombin were not dependent on PAR-1 activation, as confirmed by PAR-1 gene silencing. Thus, we propose that PAR-3 is able to signal autonomously to induce IL-8 release mediated by ERK1/2 phosphorylation, which contributes actively to inflammatory responses. Received 9 December 2007; received after revision 16 January 2008; accepted 18 January 2008     

The expression profile of TLR9 mRNA and CpG ODNs immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes

The potential effects of synthetic unmethylated oligodeoxynucleotides (ODN) containing CpG motifs, mimicking bacterial DNA, has never been evaluated on the immune response in the teleost fish gilthead seabream (Sparus aurata), the most important fish species in Mediterranean aquaculture. First, binding and competition studies have demonstrated that binding is saturated and promiscuous, suggesting the participation of several receptors. Moreover, leucocyte cytotoxic (NCC) activity, production of ROIs (reactive oxygen intermediates), and expression of immune-relevant genes was greatly primed by ODNs. Focusing on the mechanism, the TLR9 gene is widely distributed in seabream tissues and differently regulated in vitro by several stimuli. Moreover, and for the first time in fish, TLR9 mRNA has been detected in lymphocytes as the main cell-source. To conclude, ODNs containing GACGTT, GTCGTT (optimal for mouse and human, respectively) or AACGTT motifs are the most potent inducers of seabream immunity, whilst the involvement of TLR9 is under debate.     

基于CRISPR/Cas9的毛果杨bHLH106转录因子的功能研究

目的 采用CRISPR/Cas9基因编辑系统创制毛果杨(Populus trichocarpa)bHLH106(Basic Helix-Loop-Helix 106)基因的突变体,分析植株的表型特征,初步揭示PtrbHLH106基因在毛果杨木材形成过程中的功能。方法 基于前期对毛果杨野生型(WT)茎干的不同细胞类型(形成层、木质部和韧皮部细胞)RNA-seq数据,克隆得到一个在形成层及木质部较高表达的bHLH基因PtrbHLH106。采用CRISPR/Cas9基因编辑技术创制毛果杨PtrbHLH106的功能缺失突变体。对生长60、90、120 d的毛果杨ptrbhlh106突变体和WT植株进行表型观察;对生长120 d的植株各茎节进行石蜡切片,利用甲苯胺蓝染色观察并进行细胞统计分析。结果 获得毛果杨ptrbhlh106突变体;与WT相比,突变体植株的株高、地径无明显差异;在整个测量的生长周期中,第8茎节长度有缩短的趋势,茎节数量有增加的趋势;形成层细胞层数有增加的趋势但差异不显著,导管细胞孔径显著增大,纤维细胞数量显著减少。结论 ptrbhlh106突变体与WT植株在导管孔径和纤维细胞数量上存在差异,初步证明PtrbHLH106基因参与了调控毛果杨次生木质部的发育。     

TYJL-TR9型微机联锁系统维护探讨

本文在介绍TYJL-TR9型三取二容错计算机系统的组成、简单工作原理后,针对其存在的故障,重点提出了判断和处理故障的方法和措施。通过系统维护中的一些经验,制定相应设备维护管理办法,从而保证设备的运行质量,提高运输效率。     

丫啶－9－羧酸的合成

采用二苯胺为原料合成了丫啶－９－羧酸，研究了９－甲基丫啶和丫啶－９－羧酸的制备反应条件，提高了各步反应产率。     

抗肿瘤药物—9-氨基吖啶和对-氨基水杨酸甲酯衍生物的合成(Ⅳ)

本文选择9-氨基吖啶及对-氨基水杨酸甲酯为载体合成了8个新的植物生长控制剂衍生物,分别为9-(对-氯苯氧乙酰氨基)吖啶(Ⅰ),9-(2′,4′-二氯苯氧乙酰氨基)吖啶(Ⅱ),9-(2′,4′,5′-三氯苯氧乙酰氨基)吖啶(Ⅲ),9-(α-萘乙酰氨基)吖啶(Ⅳ),4-(对-氯苯氧乙酰氨基)水杨酸甲酯(Ⅴ),4-(2′,4′-二氯苯氧乙酰氨基)水杨酸甲酯(Ⅵ),4-(2′,4′,5′-三氯苯氧乙酰氨基)水杨酸甲酯(Ⅶ)和4-(α-萘乙酰氨基)水杨酸甲酯(Ⅷ)。给出了上述化合物的核磁共振和质谱数据,确证它们化学结构。初步抗癌试验结果表明化合物Ⅰ、Ⅱ、Ⅲ、Ⅴ和Ⅵ(在浓度100μg/ml下)对低分化鼻咽癌上皮细胞株(CNE_2)的生长有明显的抑制作用(IR>50％),其中Ⅱ和Ⅲ的抑制率在90％以上。