全长人PPAR-γ的表达、纯化与结构确证

目的建立表达、纯化结构完整的全长人PPAR-γ的方法。方法构建pReceiver-B01-PPAR-γ质粒并转化E．coliBL21（DE3）细胞,诱导表达重组蛋白,优化细胞生长条件;利用亲和色谱和尺寸排阻色谱纯化重组蛋白;胶内酶解重组蛋白,用离子阱质谱仪分析二维AgilentHPLC-Chip纯化的酶解片段;基于MS／MS搜索IPI、Swiss．Prot、NCBInr和MSDB数据库,鉴定重组蛋白。结果在优化的细胞生长条件（TB介质、37℃、0．8mMIPTG、诱导3h）,从每升TB中可获得280mg重组蛋白;两步纯化后,获得176mg、纯度为95％的均质重组PPAR-γ蛋白;经质谱分析和搜索蛋白质数据库,获得均匀地分布在整个PPAR-γ多肽链中的33个阳性肽段,覆盖率为60％,表明重组蛋白为结构完整的全长人PPAR-γ。配体结合活性位点S289、H323、H449和Y473无突变,保证全长人PPAR-γ与配体结合的生物学活性。结论本文所建立的方法能成功用于大量表达结构完整的全长人PPAR-γ,有利于进一步的结构、功能研究和活性配体的筛选。     

Histone methylation and ubiquitination with their cross-talk and roles in gene expression and stability

Methylation of lysine residues of histones is associated with functionally distinct regions of chromatin, and, therefore, is an important epigenetic mark. Over the past few years, several enzymes that catalyze this covalent modification on different lysine residues of histones have been discovered. Intriguingly, histone lysine methylation has also been shown to be cross-regulated by histone ubiquitination or the enzymes that catalyze this modification. These covalent modifications and their cross-talks play important roles in regulation of gene expression, heterochromatin formation, genome stability, and cancer. Thus, there has been a very rapid progress within past several years towards elucidating the molecular basis of histone lysine methylation and ubiquitination, and their aberrations in human diseases. Here, we discuss these covalent modifications with their cross-regulation and roles in controlling gene expression and stability. Received 24 September 2008; received after revision 21 November 2008; accepted 28 November 2008     

用GEP实现复杂函数的自动建模

GEP是一种新颖的遗传算法,在函数建模的应用中取得良好的结果.给出计算有效基因长度的伪代码,结合GRCM方法阅读基因,快速计算出染色体的适应值.在算法中增加了参数估计模块,用GEP得到较好模型后,用参数估计模块进行参数优化,试验显示这种混合的GEP方法比传统的最小二乘法、神经网络以及遗传程序设计等方法具有更好的性能.     

DNA methylation of fly genes and transposons

The use of anti-5-methylcytosine antibodies in affinity columns allowed the identification of methylated sequences in the genome of Drosophila melanogaster adults. In view of the presence of transposable elements amongst the identified sequences, it has been suggested that DNA methylation is involved in transposon control in the fly genome. On the contrary, a reanalysis of these data furnishes several intriguing elements that could raise new questions about the role that DNA methylation plays in the fly genome. The aim of the present paper is to discuss some features that emerge from the analysis of the identified methylated sequences. Received 26 January 2006; received after revision 8 May 2006; accepted 2 June 2006     

论戏曲之"教化"——焦循关于"花雅之争"的侧重

焦循的戏曲理论著作<花部农谭>、<剧说>,尤为赞赏花部戏曲的重要价值,在中国戏曲批评史上产生了重要影响.焦循认为戏曲应具有示范作用,应重视观赏与演出紧密结合,并能同诗歌一样"言志",而某些优秀的花部戏曲确实符合他的审美标准.     

中国汉画像石与古埃及石刻艺术的比较

汉画像石艺术是我国汉代独特的艺术形式，对我国后来的艺术产生过很大的影响。古埃及石刻艺术在世界上也独具艺术魅力。总结他们的艺术表现手法及特征，我们发现，虽然我国汉代画像石刻艺术与古埃及石刻艺术相差千年，但他们的表现手法及艺术特征有很多极其相似之处；而且形成这些艺术的社会背景、宗教思想也有着一些相似的地方。当然它们的不同之处也有很多，本文就此进行分析比较。     

情感表达的跨文化多模态感知研究

视频和音频2个模态的信息在交际过程中起着至关重要的作用,该文研究关注的问题是情感表达中不同文化背景的听者对情感的感知(解码)与这2个模态(面部表情和情感声音)的关系。发音人为中国人,录制她的5个语句的7种情感(6种基本情感+中性情感)的音频和面部表情,制作3类刺激:只有音频信息(A-only)、只有面部视频信息(V-only)以及视频和音频同步信息(AV-Congruent)。听辨人为10名不会日语的中国人和10名不会汉语的日本人。对听辨结果分析发现,面部视频信息的加入有益于情感的感知,视频和音频信息在跨文化的情感解码中起着不同的作用,与情感的唤醒度有密切的关系,相关距离空间分布模式说明听辨结果具有稳定性,跨文化的情感感知存在普遍的心理基础。