A gelatin sponge model for studying tumor growth: quantitation of tumor cells and leukocytes in the CHO tumor

Summary A gelatin sponge model system for tumor cell inoculation and retrieval of tumor-associated leukocytes is described. Gelatin sponges pre-implanted in nude mice harboring tumorigenic Chinese hamster ovary cells (line CHO) were examined at 2 and 11 days after injection of tumor cells for tumor cell content and leukocyte accumulation after digesting the sponge matrix in collagenase solution.The data indicate a progressive influx of host cells consisting primarily of macrophages, neutrophils and lymphocytes. The total number of viable tumor cells as well as the fraction of surviving tumor cells with clonogenic potential also increased with tumor age. Blank sponges not harboring tumor cells elicited an inflammatory response in the animals which did not change appreciably with length of sponge residence. However, when the sponges were harboring tumor cells, the accumulation of host leukocytes far exceeded that which occurred in blank sponges. This observation suggests a host response directed toward the tumor which is absent in animals bearing blank sponges. Apart from providing anchorage for injected cells, the gelatin sponge, by virtue of its digestibility in collagenase, makes possible the easy retrieval and precise quantitation of tumor-associated host cells.Supported by the United States Department of Energy and National Institutes of Health Grant P41-RR01315.     

葡萄幼胚培养技术的研究(Ⅰ)——激素对胚培养的影响及培养基的筛选

选用巨峰、里扎马特和无核白葡萄的幼胚接种在附加了GA3、IAA、6-BA等的不同激素水平的MS培养基上进行培养,结果表明花后60d的巨峰和花后50d的无核白的幼胚在附加GA30.5mg.L-1、IAA2.0mg.L-1、6-BA0.5mg.L-1的培养基上分别得到了44.0±8.9%和96.0±8.9%的发育率与31.9±6.4%和37.5%的成苗率;花后60d的里扎马特幼胚在附加GA30.5mg.L-1、IAA0.5mg.L-1、6-BA0.5mg.L-1的培养基上得到了76.0±8.9%的发育率和63.5±5.5%的成苗率.筛选出了适合不同品种的最佳胚培养、萌发以及成苗培养基,为葡萄新品种的选育提供了新的方法与技术依据.     

发酵法生产番茄红素培养方法的改进及优化

研究了应用三孢布拉氏霉菌生产番茄红素的发酵工艺。针对菌种生长能力的降低,首次采用了孢子悬液直接接种的方式,并对菌种孢子的生长条件进行了优化。另外,提出了直接发酵培养的一级培养工艺,该工艺稳定、简便,不仅提高了番茄红素的产量,而且缩短了发酵培养时间。