淋巴细胞白血病患者CD3基因表达模式的变化

目的:了解T细胞受体信号传导分子CD3γδεζ基因在T和B细胞白血病病人中的表达特点.方法:采用SYBR Green Ⅰ荧光定量PCR和相对定量分析法检测32例淋巴细胞肿瘤病人:急性B淋巴细胞白血病(B-ALL)12例、慢性B淋巴细胞白血病(B-CLL)9例、急性T淋巴细胞白血病(T-ALL)11例及10例健康人外周血...     

浅谈数字图书馆员信息素质的要求与培养

论述了数字图书馆的特点及图书馆员角色的转变,指出数字图书馆对图书馆员的信息素质要求,提出了培养数字图书馆员的信息素质的一些建议。     

Cardiovascular development: towards biomedical applicability

Advances in our understanding of cardiac development have fuelled research into cellular approaches to myocardial repair of the damaged heart. In this collection of reviews we present recent advances into the basic mechanisms of heart development and the resident and non-resident progenitor cell populations that are currently being investigated as potential mediators of cardiac repair. Together these reviews illustrate that despite our current knowledge about how the heart is constructed, caution and much more research in this exciting field is essential. The current momentum to evaluate the potential for cardiac repair will in turn accelerate research into fundamental aspects of myocardial biology.     

从方法到策略———略谈网络时代阅读教学观念的转变

针对当今蓬勃发展的网络阅读,应从传统的方法论研究上升到阅读策略的运用.分析了定向、激活、质疑和监控四大策略的内涵和操作.倡导学生阅读宜从重接受理解转到重批判建构,从而为思想积淀和知识创新提供切实可行的方法与途径.     

激光诱变生物效应机理探讨

提出在激光生物工程中应如何预选激光参数,并对激光诱变生物效应的机理进行了探讨.     

Introns in higher plant genes

The intron is an important component of eukaryotic gene. Extensive studies have been conducted to get a better understanding of its structure and function. This paper presents a brief review of the structure and function of introns in higher plant genes. It is shown that higher plant introns possess structural properties shared by all eukaryotic introns, however, they also exhibit a striking degree of diversity. The process of intron splicing in higher plant genes involves interaction between multiple cis-acting elements and trans-acting factors, such as 5′ splicing site, 3′ splicing site and many protein factors. The process of intron splicing is an important level at which gene expression is regulated. Especially alternative splicing of intron can regulate time and space of gene expression. In addition, some introns in higher plant genes also regulate gene expression by affecting the pattern of gene expression, enhancing the level of gene expression and driving the gene expression.     

基于多维伪F统计量的基因表达动态聚类分析方法研究

K-均值聚类分析算法是一种广泛应用于基因表达数据聚类分析中的迭代变换算法，它通过指定类别数K-基于给定的聚类目标函数，并采用迭代更新的方法，使得最终的聚类结果的目标函数值为极小值，达到较优的聚类效果。针对K-均值聚类分析算法存在参数依赖性强，且在整个聚类过程中类的数目无法改变的缺点，引入动态调整聚类个数的思想和多维伪F统计量，提出了一种基于多维伪F统计量的基因表迭动态K-均值聚类算法。实验结果表明该算法可以动态调整聚类个数，给出最佳聚类数目，从而获得较好的聚类质量．     

水稻基因表达谱分析寡核苷酸芯片制备的研究

 首先有目的地搜集了50个水稻花序相关基因,进行了探针的设计、筛选及合成纯化,用点样仪以微阵列的形式将其点于醛基化的玻璃片上;将3个不同生长阶段的水稻花序材料的总RNA经荧光标记反转录后与寡核苷酸芯片进行杂交.用ScanArray3000对获得的表达谱进行扫描分析显示,芯片图像背景均匀,信号清晰.用ImaGene4.0软件对表达谱分析表明,候选基因在水稻花序3个不同发育阶段的材料中,表达水平有显著差异.为进一步进行水稻寡核苷酸芯片的制备及应用奠定了基础.     

5种细胞周期调控基因在小鼠生殖细胞发育过程中的表达研究

 以成熟(10周龄以上)的昆明种正常小鼠的精巢和卵巢为材料,利用地高辛标记的基因探针进行组织切片上的DNA-mRNA分子原位杂交,研究了PCNA,cdc2,cyclin D1,p2 1和 p16 5种细胞周期调控基因在生殖细胞发育过程中的表达.结果表明:PCNA基因在睾丸组织的精原细胞和精母细胞中有强杂交信号,而在雌性生殖细胞及滤泡细胞的发育过程都没有杂交信号;cyclin D1,cdc2,p2 1,p16基因在生殖细胞的发育过程中都没有,表明这些基因并没有参与小鼠生殖细胞的生长和分化调控.这些事实表明在生殖细胞发育过程中,控制细胞增殖和增殖抑制的基因与培养细胞有不同的机制,它们可能采用了不同的调控系统.