Specific anti-tumor effect induced by attenuated Salmonella typhimurium vaccine expressing extracellular region of vascular endothelial growth factor receptor 2

The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors.     

绿洲-荒漠生态系统演化因素及其非线性探讨——以新疆为例

对立统一于干旱区的绿洲—荒漠生态系统在降水等自然因素和人类活动等人为因素的影响下不断进行着演化.从长期看,这一演化过程具有不可预测性,短期内却有细微可预见性.现如今在绿洲扩展同时,荒漠也在扩展,整体趋势仍是荒漠在扩展.为维持绿洲的稳定性,促使绿洲—荒漠系统处于一种稳定的发展趋势,本文从分析绿洲-荒漠这对对立统一的生态系统入手,在探讨引起这对生态系统演化的自然因素和人为因素的基础上,确定了自然因素中的降水和人为因素中的政府调控行为为演化的主导因素,初步探索了促使这对生态系统统演化的主导因素作用所具有的非线性关系,这对维持绿洲的稳定性,抑制荒漠扩展有一定的实际意义.     

升格后院校图书馆影响电子资源利用和发展因素的探讨

阐述了学校升格后电子资源对图书馆发展的影响以及分析了目前影响升格后院校图书馆电子资源利用和发展的因素,并提出了相应的改善建议和意见。     

椭圆偏振光理论中一个问题的证明

本文从椭偏光合矢量旋转角速度Ω的表达式出发，对只有沿椭圆长、短轴方向的椭偏光合矢量间的相位差才为的难点给出了证明     

上皮生长因子预防心脏术后心包粘连的实验及临床研究

目的探讨上皮生长因子(EGF)对心脏术后心包粘连的预防作用.方法术中对犬心包内喷洒上皮生长因子3个月后,观察心包粘连情况;选先天性心脏病、房缺及室缺病人40例,术中心包内喷洒上皮生长因子,术后6个月、12个月经CT检查,判定心包粘连、增厚结果.结果在动物实验和临床研究中,实验组粘连程度显著低于对照组(P<0.01).结论 EGF心包内应用有明显预防心脏术后心包粘连的作用.     

透湿过程中织物的润湿和散湿作用

采用蒸发和吸湿法测量多种织物的透湿速率，并且仔细研究用蒸发法时，织物润湿程度在透湿过程中的变化，发现织物在透湿过程中的润湿和放湿，以及织物的表面性状是影响其放湿速率的非常重要的因素。     

偏心因子数据的计算方法研究进展

从偏心因子(acentric factor)的定义出发，分析了ω数据的确定方法和获取数据的手段，评述了国内外ω计算方法的近期进展，包括对应态蒸气压关联方程法、对应态基团贡献法(CSGC)、图论法及拓扑法等，指出了各种方法的优缺点及最新的计算发展方向。     

一阶线性非齐次微分方程求解方法的讨论和一个简化的求解方法

本文证明了一阶线性非齐次微分方程y＇+P(x)·y=f(x)三个求解方法:参数变易法、积分公式、积分因子法之间的等同关系.从参数变易法中引出一个简化的积分公式.事实上此公式恰是对用参数变易法解高阶线性非齐次微分方程时对n=1的拓广.最后以实例验证此公式的简易.     

接触载荷作用下的表面裂纹分析

用边界积分方法分析了表面裂纹在接触载荷作用下的张开位移和应力强度因子，该方法将埋在无穷大弹性介质中裂纹模拟为连续分布的位错环，根据两个位错环之间的相互作用能可以得到弹性体的应变能，对弹性体的势能取极值，可以得到关于裂纹张开位移的边界积分方程，通过把半空间的边界模拟成一个包含在无穷大弹性介质中大裂纹，该方法能用已有的边界积分方法很好的处理具有任意表面形状的表面裂纹，文中算例分析了不同倾角的表面裂纹在法向和切向接触载荷作用下，裂纹尖端的应力强度因子，其结果对于分析路面表面裂纹的扩展具有重要意义。     

延长光通信传输距离技术探讨

随着光纤传输技术的不断发展,在传输领域中光传输已占主导地位。光纤存在巨大的频带资源和优异的传输性能,是实现高速、大容量传输的最理想的传输媒质。对光纤传输的要求是传的距离越远越好。从光纤传输距离的计算方法入手,结合影响传输距离的几个因素来分析可以从哪些方面入手来延长光纤通信的传输距离。