微生物膜研究方法和潜在的控制技术

在自然界中,微生物的生物膜模式对其生命循环具有十分重要的意义,已成为当前有关微生物学研究的热点之一,对其研究已引起越来越多的科学工作者的兴趣.然而,由于缺乏针对微生物膜结构的复杂性、多变性和相关的生理生化活性的高效的方法使研究工作受到限制.本文结合微生物膜研究的最新进展,介绍了几种研究微生物膜的发生装置以及检测技术,并概述了几种潜在的控制和去除微生物膜的方法.     

育肥猪棉酚中毒的诊治报告

综合来看，主要应该以预防为主，在此基础上进行严格的去毒措施，在治疗的过程中治疗原则应该以止泻、消炎、保肝、增强解毒功能和机体抵抗力为主。做到及早确诊，以便采取合理的防治措施。本文的是笔者多年的从医心得。     

银染巢式RT-PCR检测胃淋巴结微转移方法

以Ｋｅｒａｔｉｎ１９ｃＤＮＡ的套式引物建立了巢式ＲＴ－ＰＣＲ扩增Ｋｅｒａｔｉｎ１９ｍＲＮＡ的体系及ＰＡＧＥ－银染检测扩增产物的方法；对扩增体系进行优化后，分析了扩增特异性及检测敏感性，并对临床样品作了初步检测．结果表明：该扩增体系具有较好的特异性，其敏感性可达１０－６μｇＲＮＡ，即可从１０５个淋巴细胞中检出１个胃癌细胞；对临床样品检测的结果与病理检查结果相符．显示该法具有较好的可靠性．     

环氧树脂包埋块半薄切片进行免疫组化染色的方法

为应用环氧树脂包埋的电镜制样切片进行光镜免疫组化观察，探讨脱树脂试剂的浓度、新鲜度、作用时间及切片的厚度对Ｅｐｏｎｅ－８１２环氧树脂包埋的人体子宫平滑肌组织经氧化醇钠脱树胆处理后，进行ＡＢＣ法α－肌动球蛋白免疫组化染色效果的影响。结果显示新鲜配制的过饱和氧化醇钠，作用５ｍｉｎ共脱树脂效果良好且不掉片，免疫组化染色显色明显、清晰。表明脱树脂试剂的浓度、新鲜度和作用时间是实验成功的关键因素。切片厚度未     

PTEN和FHIT在口腔鳞癌中的表达及意义

目的：检测抑癌基因VIEN、FHIT在正常口腔黏膜上皮和口腔鳞癌中的表达情况，并探讨其与肿瘤组织学类型的关系。方法：采用SP法免疫组化方法检测62例口腔鳞癌中和12例正常口腔黏膜中FHIT、PTEN的蛋白表达。结果：在正常口腔黏膜中PTEN均为强阳性表达(12／12)，在口腔鳞癌中24．2％(15／62)表现为PTEN蛋白表达的缺乏或减少；而FHIT在正常口腔黏膜中也均为强阳性表达(12／12)；在口腔鳞癌中17．7％(11／62)表现为FHIT蛋白表达缺乏或减少。结论：PTEN、FHIT在口腔鳞癌的发生过程中起着一定的作用。     

Spontaneous formation of tubular microstructure vesicle from PTA／SDS system

Spontaneous vesicle formation and transformation of vesicle to tubular microstructure have been observed in aqueous mixtures of tri-(n-dodecyl-dimethyl-hydroxy-propylammonium chloride) phosphate (PTA) and sodium dedecyl sulfate (SDS), which is supported by negative-staining transmission electron microscopy (TEM), scan electron microscopy (SEM) and dynamic light scattering (DLS). Spherical vesicles aggregate to tubular microstructures with their diameters and lengths respectively of about 100 and 7000 nm in the freshly prepared sample at the molar ratio of PTA/SDS=3/7 and a total surfactant concentration of 0.01 mol/L. The diameter, the length and the quantity of tubular structures all increase with the increase of the total concentration of surfactant and the sample aging.     

The expression of Quox-1 gene in the embryo development ofAndrias davianus

Two set ofAndrias davidanus fertilized eggs and its seedlings were got by means of artificial reproduction. The sections of the embryo ofAndrias davidianus were made by histological method. The expression of homologous sequences of Quox-1 gene has been studied by immunohistochemical method with special antibody. Results show that the expression of homologous sequences of Quox-1 gene was detected in the epidermis of blastula and gastula. The highly expression was investigated in the epidermis at the neural tube phrase and the tail bud stage. Deng Fengjiao: born in October, 1954, Associate Professor     

Ultrastructural localization of microfibrillar fibulin-1 and fibulin-2 during heart development indicates a switch in molecular associations

The microfibrillar proteins fibulin-1 and fibulin-2 were previously identified as prominent components of the endocardial cushion tissue (ECT) during heart development and shown to persist in adult valves and septa. Immunogold staining has now been used to compare their localization in embryonic (days 9–11) and adult mouse heart with that of fibronectin and the chondroitin sulphate proteoglycan versican. All four proteins were deposited in the ECT, which consists of a hyaluronan-rich, mainly unstructured matrix, but were barely detectable in myocardial basement membranes or within endocardial cells. Digestion with hyaluronate lyase selectively released the fibulins and versican but not fibronectin from the ECT. Yet neither of the two fibulins bound to hyluronan in solid-phase assays, in contrast to versican. In the adult heart valve, all four proteins could be detected close to cross-striated collagen fibrils or microfibrils, but only versican was lost upon exposure to hyaluronate lyase. The data indicate that fibulins are associated with the hyaluronan-matrix of ECT through a bridge of versican, but that this association changes upon valve development to another supramolecular, presumably microfibrillar organization based on fibronectin and/or fibrillins. Received 3 April 1998; accepted 8 April 1998     

人胚组织块切前整体染色法

本文介绍了人胚组织块切前整体染色方法。此法比常规石蜡切片法优越,它减少了操作过程,具有省时、省工、节省药品的特点。