疣荔枝螺消化系统甲硫氨酸脑啡肽的免疫组织化学定位

采用免疫组织化学的方法（SABC法）对疣荔枝螺消化系统各器官中甲硫氨酸脑啡肽（met—Enk）进行了定位研究,疣荔枝螺的吻、唾液腺、食道、食道腺、胃、小肠、肝脏、直肠中均有met—Enk存在．其巾唾液腺、肝脏呈met—Enk强阳性,吻、食道、食道腺、胃、小肠、直肠呈met-Enk阳性．在各器官的不同组织中met—Enk阳性义有不同：腺体和消化道的上皮组织和结缔组织呈强阳性,消化道的肌肉组织呈阳性或弱阳性．met—Enk在消化系统各器官及不同组织中的分布密度不同可能与各部位功能不同有关．     

Immunohistochernical localization of IAA in graft union of explanted internode grafting

The dynamic changes of IAA in graft union of explanted internode autografting of Cucumber (Cucumis sativus Linn.) have been investigated using the immunohistochemical localization technique. It is shown that the efficiency of IAA fixation using lyophilization-gas fixation is higher than that using liquid chemical fixation. In contrast to few silver particles and no significant changes during the development of graft union cultured in hormone-free medium, more silver particles in graft union and significant changes of IAA related to graft union development have been found when graft union was cultured in medium supplemented with appropriate hormones. The fixation procedure of plant hormones and the roles of IAA in graft union are discussed.     

Application of FLUPD-DD-PCR to the study of mRNA expression of glioma cells cultured under the condition of serum starvation

Fluorescently labeled universal primer directed differential display polymerase chain reaction technique (FLUPD-DD-PCR), an improved DD-PCR, is reported for the study of mRNA expression of glioma cells cultured with the serum starvation. The fluorescently labeled universal primer (FLUP) facilitates analysis of PCR products on an auto-sequencer. Compared with the traditional DD-PCR, FLUPD-DD-PCR has advantages of separation of target bands and measurement of mRNA expression quantitatively by adopting Cy5-labeled universal primer. And then the silver staining method has been used to recover the bands for use as template in re-pcr. In this study, 4 differential ESTs, of which 3 are novel ESTs, have been obtained. This work indicates some special novel genes, which can be induced to express under serum absent condition, are involved in coping with serum starvation stress on glioma cells growth.     

青海玉树多目涡虫咽部组织结构的显微观察

利用连续石蜡切片(包括水平切、矢状切和横切)技术,H.E染色和光镜观察的方法,对青海玉树多目涡虫咽部的组织结构进行了初步的研究,结果表明:咽壁包括三层结构,外层由单层柱状上皮构成,似合胞体,上皮细胞游离面可见三层嗜酸性带状结构,其中,外层带呈刷状;咽壁的中层分为内侧发达的环肌和外侧较薄的纵肌两部分;邻接咽腔的内层上皮在游离面呈指状突起,未见上皮细胞间界限.     

青海玉树多目涡虫精巢组织结构的显微观察

利用连续石蜡切片(水平切、矢状切、横切)及H.E染色方法,对青海玉树多目涡虫精巢的组织结构进行了初步研究.结果表明:在光学显微镜下,多目涡虫精巢的组织结构与哺乳类睾丸中曲细精管的结构类似,但也有一些差别.提示:精巢的组织结构在动物的系统演化过程中是比较保守的,通过对不同进化类群动物精巢的组织结构进行比较研究,也许有助于对动物系统演化的探究.     

两种不同示踪剂用于蛋白质与抗原分子微阵列信号检测的对比研究

以氧化型琼脂糖凝胶膜为基片，制作蛋白质与抗原分子微阵列，建立了检测固相人IgG,游离IgG和自身抗体分子的免疫学测定模式，分别以辣根过氧化物酶－二氨基联苯胺一双氧水（HRPDAB－H2O2）和胶体金－硝酸银－对苯二酚2种示踪系统作为蛋白质与抗原分子微阵列信号显示剂，并对其作用的原理与特点进行讨论，检测结果发现基于胶体金的免疫金银染色法（IGSS）比固相酶免疫测定法（EIA）敏感，2种示踪系统对固相人IgG的最低检出量分别为0．05ng和0．5ng；而对血清游离IgG的最低检出量IGSS法比EIA法至少高出10倍，两法用于自身抗体的测定亦表现出较好的重复性，在14例HCV感染者中，自身抗体的总体检出率分别为38．6％和31．4％，结果具有显著的同步性，故同时辅以2种示踪剂并用于阵列芯片的联合测试，有助于提高自身抗体的总体检出水平。     

Replication pattern of double minutes derived from an insect cell line

Summary The DNA replication pattern of double minutes derived from an established cell line ofAedes albopictus is described. Although the vast majority of double minutes replicate semiconservatively once during the S phase, some double minutes appear to exhibit different pattern(s). Two theories are suggested as possible explanations of our findings.This work was supported by a grant from the Whitehall Foundation, Inc. awarded to A.B.M.     

快速高通量测定三角褐指藻油脂含量

为了实现三角褐指藻细胞内油脂含量的快速高通量测定,探索了尼罗红荧光染色法检测其油脂含量的检测条件.采用96孔板并配合酶标仪考察三角褐指藻的预处理液的种类及其用量、染色液尼罗红的用量、染色温度、染色时间以及细胞浓度对荧光强度的影响,确立最优染色条件,并研究荧光强度与油脂含量之间的对应关系.结果表明,TritonX-100终浓度为0.05%时,通透效果最佳,每100μL藻悬液中加3μL尼罗红染液(0.1mg/mL),温度40℃,时间10min,细胞浓度低于3×106个/mL时,荧光强度(y)与油脂含量(x)之间呈现良好的线性关系:y=927.37x+15.913,R2=0.9247.方法优化后,90min即内可完成对96个样品的处理及分析,极大提高了分析的通量.     

一种分析有丝分裂期蛋白的新方法

介绍了一种分析细胞有丝分裂任一时期蛋白质组成及各时期蛋白动态变化的方法，该法将细胞的显微分离，微量蛋白电泳和银染结合起来，通过显微分离可以获得无期它时期细胞污染的任一时期细胞，通过微量蛋白电泳和银染，可以分析其蛋白组成，并通过显微分离100个小麦根尖末期细胞证明了其可行性。     

细菌芽孢染色方法比较的研究

芽孢是微生物的重要结构,对于微生物的分类有着十分重要的作用。目前,在芽孢染色上虽有不少方法,但仍存在染色效果不明显的缺点。对三种芽孢染色法进行了比较研究,结果发现：方法三着色强,观察清晰,染色效果更理想,而且该方法快速、有效、简便易行,更适于在实验教学中应用。