人胎儿骨髓间充质干细胞的分离及生物学鉴定

通过原代细胞培养,从引产胎儿骨髓组织中分离干细胞,然后进行生物学鉴定,旨在体外建立培养胎儿骨髓干细胞的有效方法,为进一步研究干细胞奠定基础．本研究对四个月的引产胎儿骨髓组织进行原代细胞培养,采用贴壁筛选法,在含有15％胎牛血清的L-DMEM／IMDM(1:1)混和培养液中培养,7 d后细胞可长满瓶底．显微镜下观察,细胞形态均一,呈长梭形．传代后,在8代以内的细胞贴壁能力较强,生长速度较快．将其命名为BMMS-03．在第3代时,对培养的细胞进行了于细胞标志物的生物学鉴定,采用流式细胞仪对经免疫荧光染色的细胞进行检测．结果显示:97．2％的细胞呈CD105阳性反应,66．0％的细胞呈CD106阳性反应, 9．2％的细胞呈CD34阳性反应．阴性对照组阳性反应为0．5％．生物学鉴定的初步结果提示,从胎儿骨髓组织中分离培养成功的细胞为骨髓间充质干细胞,其细胞形态学特征、CD105 、CD106 和CD34-的检测结果均符合间充质干细胞的特征．本研究成功地建立了体外培养胎儿骨髓间充质干细胞的有效方法,所获得的间充质干细胞纯度较高,增殖较快,适用于干细胞生物学和组织工程学的研究．     

复合光伏阵列的优化匹配研究

为了提高光伏发电效率,降低独立光伏发电系统成本,将强光特性的单晶硅电池和弱光特性的非晶硅电池复合应用在发电系统中。通过对太阳电池的I-V特性分析,构建复合光伏阵列输出功率与辐照强度、温度之间的关系,通过辐射量、日照时数等气象参数对定容量的独立光伏发电系统进行了光伏复合组件的优化研究。     

CdCl2诱导秋粘虫细胞(Sf9)凋亡的初步研究

以秋粘虫（Spodoptera frugiperda Sf9）的细胞为材料，用不同浓度的CdCl2，通过Giemsa染色的形态学方法，研究其细胞凋亡．结果表明，在重金属离子的胁迫作用下，不同浓度的重金属离子致使细胞死亡率不同；其急性损伤域为136μmol／L；20μmol／L CdCl2作用6h后，Sf9细胞出现了凋亡小体．     

酵母粉对果蝇精子发生之影响

目的探讨酵母粉对果蝇精子发生各时期的影响。方法野生型黑腹雄蝇按2-3-3法处理,置于每管酵母粉含量为0,5 mg/vial,10 mg/vial,15 mg/vial培养基中培养,统计精子发生各阶段精细胞数目。结果随着酵母粉量的增加,精子发生在各阶段细胞数量增加,在不同处理组中雄蝇在其精子发生各阶段中,以第3天形成精细胞数量最多,其中10 mg/vial和15 mg/vial最为显著。酵母粉量的增加,在精细胞阶段促进精细胞数量增加最为显著,成熟精子阶段也有增加,精母细胞阶段精细胞数量呈增加后逐渐减少趋势。结论酵母粉可促进果蝇的精子发生,特别在精细胞阶段,促使精细胞数量增加,高峰期明显提前并持续延长形成时间。     

连续/离散混合型制造系统的生产过程虚拟仿真建模

离散事件仿真建模被广泛应用于制造系统的设计及运行控制,但是很多制造系统兼有离散系统和连续系统的特点。提出一种将离散事件与连续系统结合起来的仿真建模方法,以支持动态展现这类制造系统的生产过程。该方法的主要思想是,主机内部的机构运动主要采用连续系统进行仿真建模,而主机之间的加工顺序则利用离散系统来构建其仿真模型,然后由统一的仿真管理器管理。论文详细描述了虚拟仿真引擎中的仿真时钟推动机制,并用这一方法建立了面向啤酒灌装生产线生产过程的虚拟仿真模型,说明了这一方法的可行性。     

Surface molecules regulating rolling and adhesion to endothelium of neutrophil granulocytes and MDA-MB-468 breast carcinoma cells and their interaction

The extravasation of leukocytes and tumor cells is a multi-step process with the involvement of various adhesion molecules depending on the three steps rolling, adhesion, and diapedesis. We have developed an in vitro model, by which we investigated the rolling and adhesion of neutrophil granulocytes and MDA-MB-468 human breast carcinoma cells to lung endothelial cells under physiological flow-conditions. We found that norepinephrine had an inhibitory function on the fMLP-promoted adhesion of neutrophil granulocytes due to a down-regulation of β2-integrin. Furthermore, neutrophil granulocytes serve as linking cells for the interaction of the MDA-MB-468 cells with the endothelium, which are both β2-integrin negative, but express the β2-integrin ligand ICAM-1. In addition, we show here that N-cadherin is up-regulated on the endothelial cells and on neutrophil granulocytes in response to fMLP. This up-regulation resulted in a significant increase of adherent MDA-MB-468 cells, which are also N-cadherin positive. Received 3 September 2007; received after revision 17 October 2007; accepted 22 October 2007     

Cardiovascular development: towards biomedical applicability

Advances in our understanding of cardiac development have fuelled research into cellular approaches to myocardial repair of the damaged heart. In this collection of reviews we present recent advances into the basic mechanisms of heart development and the resident and non-resident progenitor cell populations that are currently being investigated as potential mediators of cardiac repair. Together these reviews illustrate that despite our current knowledge about how the heart is constructed, caution and much more research in this exciting field is essential. The current momentum to evaluate the potential for cardiac repair will in turn accelerate research into fundamental aspects of myocardial biology.     

From endoderm to pancreas: a multistep journey

The formation of the vertebrate pancreas is a complex process that typifies the basic steps of embryonic development. It involves the establishment of competence, specification, signaling from neighboring tissues, morphogenesis, and the elaboration of tissue-specific genetic networks. A full analysis of this multistep process will help us to understand classic principles of embryonic development. Furthermore, this will provide the blueprint for experimental programming of pancreas formation from embryonic stem cells in the context of diabetes cell-therapy. Although in the past decade many studies have contributed to a solid foundation for understanding pancreatogenesis, important gaps persist in our knowledge of early pancreas formation. This review will summarize the current understanding of the early mechanisms coming into play to pattern the "pre-pancreatic" region within the endoderm and, gradually, specify the pancreatic tissue.     

高效液相色谱测量啤酒中氨基酸

本文建立了测量啤酒氨基酸的有效方法,该方法使用高效液相色谱采用梯度洗脱测定啤酒中的氨基酸,结果回收率为99.2%,RSD为2.01%,表明本方法精密度高,稳定性好,可作为检测啤酒氨基酸有效方法。     

发酵型梨汁酒酿造工艺研究

梨是人们日常生活中的一种主要水果,将梨汁进行酿造,得到的梨汁酒既有酒的醇香又有梨的保健功能,对发酵型梨汁酒酿造工艺进行了研究,起始糖度20％左右,最佳发酵温度20℃,发酵液pH为5,发酵时间7d,该条件下发酵的梨汁酒颜色金黄,透光率高,口感较好．同时也对梨汁酒工业化做了展望．