雌激素受体ERβ配体结合区的异源表达与纯化

为研究环境化合物与雌激素受体的相互作用,在大肠杆菌中异源表达了人雌激素受体ERβ的配体结合区(hERβ-LBD)蛋白。在诱导剂(IPTG)浓度为0.2mmol.L-1,培养温度25℃,诱导时间为8h的优化表达条件下,经亲和色谱法纯化后,hERβ(LBD蛋白的产量可达236mg.L-1.     

角域内的亚纯函数

1925年 Nevanlinna 提出下述猜想:设函数 f(z)在角域{z|α≤arg(?)≤β,0<β-α≤2π}内亚纯,则(?) (1)至多除去 r 的一个测度有限的集合.本文在适当的边界条件下证明了这个猜想.     

环区原理的一个推论及其应用

本文给出环区原理在三维空间中的一个推论,它证明了满足三个条件的空间系统就满足环区原理的条件,从而存在周期轨道。利用这个推论,又得到两个有关构作空间极限环的定理。     

Nucleotide binding domains of human CFTR: a structural classification of critical residues and disease-causing mutations.

Defective function of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) causes CF, the most frequent lethal inherited disease among the Caucasian population. The structure of this chloride ion channel includes two nucleotide-binding domains (NBDs), whose ATPase activity controls channel gating. Recently, the experimental structures of mouse and human CFTR NBD1 and our model of the human CFTR NBD1/NBD2 heterodimer have provided new insights into specific structural features of the CFTR NBD dimer. In the present work, we provide a structural classification of CF-causing mutations which may complement the existing functional classification. Our analysis also identified amino acid residues which may play a critical role in interdomain interaction and are located at the NBD1-NBD2 interface or on the surface of the dimer. In particular, a cluster of aromatic amino acids, which includes F508 and straddles the two NBDs, might be directly involved in the interaction of the NBD1/NBD2 heterodimer with the channel-forming membrane-spanning domains.Received 24 May 2005; received after revision 13 June 2005; accepted 18 June 2005     

BICP0 and its RING finger domain act as ubiquitin E3 ligases <Emphasis Type="Italic">in vitro</Emphasis>

Bovine infected-cell protein 0 (BICP0) encoded by bovine herpes virus 1 (BHV-1) immediate early gene is necessary for efficient productive infection, in a large part,because it activates all 3 classes of BHV-1 genes. It also has the ability to efficiently transactivate promoters that are not derived from BHV-1. To investigate the mechanism by which BICP0 achieves these effects, we expressed and purified BICP0 and its different mutants in E. coil In vitro assays showed that both full-length BICP0 and its isolated RING finger domain induce the accumulation of polyubiquitin chains. Mutations within the RING finger region that abolish the in vitro ubiquitination activity also cause severe reductions in BICP0 activity in other assays. Based on these, we conclude that BICP0 has the potential to act as an E3 ubiquitin ligase during viral infection and its RING finger domain is necessary for this function. These strongly support the hypothesis that BICPO might influence virus infection through its ability to interact with the ubiquitin-proteasome pathway.     

电力系统动态等值中励磁系统参数聚合方法对比研究

为了减少计算量,提高分析速度,对励磁系统进行动态等值时,可采用统一的降阶标准传递函数作为等值励磁系统模型.分别使用加权平均法、离散时间最小二乘法和分段线性多项式函数(PLPF)法确定了等值励磁系统模型的参数,并将遗传算法引入等值励磁系统模型的参数辨识.重点对比了几种励磁系统聚合方法,归纳出各种方法的差异及优缺点.仿真算例表明,离散时间最小二乘法性能较好.     

结构-地基相互作用的频域计算模型

基于比例边界有限元法（SBFEM）建立了结构-无限地基相互作用分析的频域透射边界,这种透射边界精确满足Sommerfeld辐射条件.该方法的特点是可以根据精度需要从低阶到高阶逐步递推计算无限域刚度矩阵,应用简单且十分方便.针对文献中比较常用的各种无限地基计算模型的适应性进行了一定的研究分析.结合无限地基上重力坝的地震响应,对无限地基迟滞效应的影响进行了对比分析,由对比结果可以看出考虑无限地基影响时的坝体最大拉应力分布比常用的无质量地基模型的计算结果约小15%;考虑迟滞效应时最大应力计算结果比不考虑迟滞效应时的计算结果增加了约25%.     

利用频域连续性检测非合作突发通信信号

针对在非合作突发通信信号检测中,已有的各种检测方法均未能充分利用信号的频域连续性的问题,提出了一种新的检测方法,利用信号的频域连续性,在频率和带宽二维进行频域能量累积,搜索最大值并检测.理论分析和仿真均验证了此方法优于Power-Law方法 2 dB以上.     

论张志和《渔歌子》的江南话语

江南,是张志和《渔歌子》建构的核心意象。通过对历史语境的还原和文本内涵的解读,不难发现这一意象,不仅是张志葙的叙述场域,也是一种叙述话语,即江南话语,它传达出了隐逸诗人张志和在“家国同构”的认同传统下内心激烈的斗争和挣扎。     

变转速齿轮箱振动信号监测的无键相时域同步平均方法

为了克服传统时域同步平均方法应用中需要键相信号及恒定转速的要求,提出了一种不需要键相信号的变转速齿轮箱振动信号时域同步平均方法.该方法通过振动信号时频域滤波及滤波信号的瞬时相位计算来估计齿轮箱的瞬时转速,通过时原始振动信号的等角度间隔重采样来消除转速变化的影响,并用重采样信号进行了时域同步平均.变转速齿轮箱实验台的实验数据分析表明,该方法可以准确地估计变转速齿轮箱的瞬时转速,从而消除了齿轮箱转速波动对振动信号的影响.重采样信号的时域同步平均结果为齿轮监测诊断提供了有效信息.