Some of Advances on the Error Analysis in the Frequency Domain for a Digital Simulation Model

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重组人甲状旁腺激素(1-34)在大肠杆菌中的表达与纯化

将化学合成的rhPTH(1-34)基因用PCR扩增后,克隆至表达载体pET-35b( ),使rhPTH(1—34)融合于纤维素结合结构域(CBDdos)的羧基端,并得到高效表达．融合蛋白经纤维素树脂亲和层析纯化后,经Factor Xa裂解释放出rhPTH(1-34),再通过纤维素树脂亲和层析、C4反向高效液相色谱纯化得到rhPTH(1-34)纯品．每升培养液可获取3mg高纯度的rhPTH(1-34)．经质谱测定,所得样品的分子量为4117．0Da,与rhPTH(1—34)理论分子量一致．     

Horizontal gene transfer from Eukarya to Bacteria and domain shuffling: the α-amylase model

-Amylases are present in all kingdoms of the living world. Despite strong conservation of the tertiary structure, only a few amino acids are conserved in interkingdom comparisons. Animal -amylases are characterized by several typical motifs and biochemical properties. A few cases of such -amylases have been previously reported in some eubacterial species. We screened the bacterial genomes available in the sequence databases for new occurrences of animal-like -amylases. Three novel cases were found, which belong to unrelated bacterial phyla: Chloroflexus aurantiacus, Microbulbifer degradans, and Thermobifida fusca. All the animal-like -amylases in Bacteria probably result from repeated horizontal gene transfer from animals. The M. degradans genome also contains bacterial-type and plant-type -amylases in addition to the animal-type one. Thus, this species exhibits -amylases of animal, plant, and bacterial origins. Moreover, the similarities in the extra C-terminal domains (different from both the -amylase domain C and the starch-binding domain), when present, also suggest interkingdom as well as intragenomic shuffling.Received 17 October 2003; accepted 6 November 2003     

Optimization of short-pulse GPR transmit antenna

By analyzing the current distribution of Bow-Tie amerma used in short-pulse ground penetrating radar, the methods of antenna load and driving are presented in this paper to reduce strength of reflective wave both at antenna end and excitation point. The numerical simulation results show the strength oi reflective wave is smaller than -55dB comparing with the driving wave when the methods are adopted.     

A detection strategy of multi-pose face in compressed domain

In this paper, we present a strategy to implement multi pose face detection in compressed domain. The strategy extracts firstly feature vectors from DCT domain, and then uses a boosting algorithm to build classifiers to distinguish faces and non-faces. Moreover. to get more accurate results of the face detection, we present a kernel function and a linear combination to build incrementally the strong classifiers based on the weak classifiers. Through comparing and analyzing results of some experiments on the synthetic data and the natural data, we can get more satisfied results by the strong classifiers than by the weak classifies.     

同轴电缆的时域反射技术及其在工程中的应用

通过研究阶跃电压信号因电缆的阻抗变化而引起的反射规律,推导出了在多个强间断变化阻抗的情况下,阻抗的相对变化量与反射电压的关系,以及同轴电缆几何尺寸变化与电缆传输阻抗之间的关系。指出在小变形的假设下,它们之间存在着理想的线性关系。同时,举例说明了同轴电缆的时域反射技术在交通工程中的应用。     

基于流量域的校园网组网新模式

传统的互联型网络架构难以适应日趋复杂的校园网应用需求,是目前影响数字化校园建设深入发展的一个重要因素.借鉴城域网建设思路,充分考虑学校各个功能模块对网络带宽、网络安全、服务质量、策略管理等方面的要求,积极探索按流量域划分的校园网建设新模式,使各功能子网既有机联系又相对独立,杜绝冲突与碰撞,可以为广大师生员工提供更高性能、更高安全、更灵活易管理的网络应用平台,同时为数字化校园的实现打下更为坚实的基础.     

利用克立金方法预测湿地水质的变化趋势

将空间域上的克立金方法延伸到时空域中,得出当时间信息量大于空间信息量时区域化变量的无偏最优估计形式,进而给出时间域上克立金的估计形式,最后利用该方法对某湿地近几年水体中BOD-DO的未来的趋势进行了预测,实例说明该方法是一种比较稳健的预测方法.     

一种基于自相关函数的小波域数字水印算法

几何攻击是对数字水印技术最具威胁的攻击手段之一，而基于自相关函数的水印算法被认为是一种有效的解决手段，却有其局限性。研究发现，对用于评价几何攻击起很重要作用的水印的自相关峰值（以下简称AC峰值），也可从小波域提取出来。这里介绍一种小波域基于ACF的数字水印算法，该算法在考虑到AC峰值和噪声能见度的影响的同时，把一个周期性水印信号嵌入小波域。仿真实验结果表明，算法能产生更显著的AC峰值，从而提高了抗几何攻击的鲁棒性。     

二元函数极限求法中一种误解的说明

排除了对一个重要极限在求二元函数极限应用中正确性的疑虑，并在此基础上把它的结果推广到了含无理式的二元函数的极限运算上去．