Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Diamond synthesis from a system of chromium-carbide and Ni70Mn25Co5 alloy

In order to ascertain the mechanism of interaction between carbide and metallic catalyst and formation of diamond under high pressure and high temperature, and find a new method to synthesize diamond with special properties, it is necessary to investigate the reaction behavior of different carbides and metallic solvent_catalysts under high pressure and high temperature. A system of Cr-3C-2 powder and Ni 70Mn 25Co 5 alloy in weight ratio of 1∶6 was treated under 6 0 GPa and 1 500℃ for 20, 30 or 60 min respectively. X_ray diffraction of the samples indicated the Cr 3C 2 decomposed partially after high pressure and temperature treatment, and Cr 7C 3, Cr and diamond formed respectively. There was not any trace of graphite in the samples. The result suggested that the separated carbon atoms could form diamond directly without conversion process of graphite into diamond. The observation of SEI, WDX and EDX also showed that diamond crystals were synthesized in the system, which have perfect surfaces and shapes, with the average grain size of about 40 μm. The properties of the crystals are being investigated.     

火力发电厂汽机房屋盖结构选型分析

本文对汽机房屋盖结构的两种受力体系进行了技术经济比较,阐述了空间网架在我国的应用情况,介绍了平板型网架应用在火力发电厂汽机房屋盖中的可行性及经济性。根据工业厂房建筑中的传力路线,分析了用于汽机房屋盖结构的平板型网架考虑吊车水平刹车力作用时的内力分布特点及其应用,提出了汽机房屋盖结构使用平板型网架的观点     

应变速率对8090Al-Li合金拉伸性能的影响

用动态拉伸的方法研究了应变速率对 80 90 Al— L i合金拉伸性能的影响 ,应变速率的范围为 10 - 3～ 10 3s- 1。结果表明 ,合金的强度和塑性随应变率的增加而增加。试样的 SEM和 TEM分析发现 :准静态下的断裂为沿晶断裂 ;随着应变率的增加 ,断裂从穿晶准解理断裂过渡到由显微空穴聚集而成的韧窝断裂。高应变率下80 90 Al- L i合金强度和塑性的提高与其变形机制和晶界无沉淀区 (PFZ)的变化有关     

不锈钢堆焊试件熔合区充氢前后的断裂韧性研究

研究了氢对不锈钢堆焊层熔合区的断裂韧性的影响作用。发现不锈钢堆焊层熔合区的断裂韧性随着充氢时间的增加而下降,结果表明,氢是引起加氢反应器不锈钢堆焊层剥离的主要因素。     

16MnR钢中疲劳裂纹扩展的细观研究

采用显微疲劳试验方法和显微疲劳试验装置对低合金钢１６ＭｎＲ单边缺口试样进行了疲劳试验,研究了短裂纹萌生和扩展的特征。结果表明,短裂纹萌生及扩展分三个阶段,每一阶段的形貌都有其特点,在裂纹扩展后期,短裂纹的发展以裂纹密度不断增加并形成损伤区为主要特征,然后在损伤区选择一条主裂纹扩展。     

电聚合吡咯膜修饰的葡萄糖氧化酶电极

自制的铂丝电极经铂化处理后,将葡萄糖氧化酶与单体吡咯用循环伏安法共聚合到电极上,铂化后电极表面积的增大增加了固定的酶量,同时也增大了电极对Ｈ２Ｏ２的催化能力,使电极对葡萄糖的响应电流增大到７．５ｍＡ／（ｍｏｌ·Ｌ＾－１）,还测试了聚合液中酶浓度、ｐＨ值和聚合时间等因素对电极特性的影响。     

Construction of High Level Expression Cell Lines of Erythropoietin *

Ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ（ＥＰＯ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｒｅｇｕｌａｔｏｒｙｆａｃｔｏｒｓｄｕｒｉｎｇｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｍａｍｍａｌｉａｎｅｒｙｔｈｒｏｃｙｔｅ,ａｃｔｉｎｇｍａｉｎｌｙｏｎｔｈｅｅｒｙｔｈｒ...     

水泥黄土增强措施初探

在分析资料和室内试验的基础上,概括了提高水泥黄土强度有效措施,就水泥黄土硬化和外加剂对水泥黄土的作用机理进行了初步探讨。     

诱导法曲率对圆弧齿线双圆弧齿轮接触强度的影响

根据齿轮啮合原理,分析并比较了圆弧齿轮双圆弧齿轮与普通双圆弧齿轮啮合特性,通过推导这种新型齿轮齿面诱导法曲率,分析了接触强度得以提高的原因。同时用切齿法进行研究后得出,该齿轮还具有较高弯曲强度。