超高压高CO2气藏偏差系数图版扩展与应用

Standing-Katz图版法确定天然气偏差系数是目前最常用、最基础的方法之一,但存在人工读值误差大、在超高压（拟对比压力大于15）和高CO₂含量天然气使用受限等问题。设计单组分偏差系数测定实验,结合Standing-Katz图版曲线变化规律,将图版拟对比压力扩展到30,拟对比温度扩展到3.6,并将图版数字化;基于不同CO₂含量偏差系数测定实验结果,推导了考虑不同CO₂含量的天然气拟临界压力、拟临界温度计算新模型,与常规非烃校正模型相比,新模型计算精度更高,适用范围更广。进一步将新临界参数校正模型嵌入到偏差系数扩展图版中,得到不同CO₂含量的偏差系数扩展图版,该图版在南海西部超高温高压、高CO₂含量天然气偏差系数计算中误差较小（不超过3%）,应用效果良好。     

HQ70钢疲劳强度统计特性研究

在对HQ70钢疲劳寿命统计特性研究的基础上,利用疲劳寿命分布与疲劳强度分布函数间的转换关系,对HQ70钢疲劳强度分布特性进行研究,并作出疲劳寿命强度概率分布的P-s-N三维空间曲面.确认疲劳强度分布并非通常假设的正态分布,而是偏态分布,其特征函数的概率分布为标准正态分布.     

含铝高速钢中奥氏体非均匀长大的机制

M2AI和M2高速钢经一次和二次淬火后,奥氏体晶粒和未溶碳化物的定量研究表明,在高温r/f比值相同的条件下,由于淬火前原始组织的差别,可发生正常(均匀)和异常(非均匀)两类长大。根据异常粗晶形成初期的组织观察,提出了“连续-非连续”再结晶模式和“合并—推移”式的生长机制。提出了减轻或消除M2AI钢混晶的工艺原则。     

泥沙输移对长江中游水位抬升的影响

通过对长江干流宜昌至汉口河段及与洞庭湖区泥沙输移变化的综合分析,得到了三口分流分沙逐年减少是造成洞庭湖区淤积速率减小和螺山至汉口河段淤积速率增大的主要原因,也是造成长江干流监利至螺山河段水位逐年抬升、洞庭湖区的洪水调节能力大幅度下降的主要原因等结论．按照因势利导的治理原则,荆江及洞庭湖的近期治理策略应是淤积围垸减少河道淤积、疏浚河道、加固干堤．     

2,3-二羟基哌嗪与几种氨基化合物的缩合反应研究

由乙二胺与乙二醛的缩合物２,３－二羟基哌嗪,分别与甲酰胺、脲和乙二胺反应,制得２,４,７,９,１１,１４－六氮杂三环［８,４,０,０３,８］十四烷六盐酸盐四水合物（１）,２,５,７,９－四氮杂双环［４,３,０］壬－８－酮二盐酸盐－水合物（２）和２,５,７,１０－四氮杂双环［４,４,０］癸烷（３）。化合物１为未见文献报道的新化合物。化合物２的合成工艺条件优化后,反应得率与文献［１］相比有一定提高。化合物３的合成,由于将反应分为低温和高温两个缩合阶段,使反应得率提高到７７．５％。     

Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Diamond synthesis from a system of chromium-carbide and Ni70Mn25Co5 alloy

In order to ascertain the mechanism of interaction between carbide and metallic catalyst and formation of diamond under high pressure and high temperature, and find a new method to synthesize diamond with special properties, it is necessary to investigate the reaction behavior of different carbides and metallic solvent_catalysts under high pressure and high temperature. A system of Cr-3C-2 powder and Ni 70Mn 25Co 5 alloy in weight ratio of 1∶6 was treated under 6 0 GPa and 1 500℃ for 20, 30 or 60 min respectively. X_ray diffraction of the samples indicated the Cr 3C 2 decomposed partially after high pressure and temperature treatment, and Cr 7C 3, Cr and diamond formed respectively. There was not any trace of graphite in the samples. The result suggested that the separated carbon atoms could form diamond directly without conversion process of graphite into diamond. The observation of SEI, WDX and EDX also showed that diamond crystals were synthesized in the system, which have perfect surfaces and shapes, with the average grain size of about 40 μm. The properties of the crystals are being investigated.     

应变速率对8090Al-Li合金拉伸性能的影响

用动态拉伸的方法研究了应变速率对 80 90 Al— L i合金拉伸性能的影响 ,应变速率的范围为 10 - 3～ 10 3s- 1。结果表明 ,合金的强度和塑性随应变率的增加而增加。试样的 SEM和 TEM分析发现 :准静态下的断裂为沿晶断裂 ;随着应变率的增加 ,断裂从穿晶准解理断裂过渡到由显微空穴聚集而成的韧窝断裂。高应变率下80 90 Al- L i合金强度和塑性的提高与其变形机制和晶界无沉淀区 (PFZ)的变化有关     

Construction of High Level Expression Cell Lines of Erythropoietin *

Ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ（ＥＰＯ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｒｅｇｕｌａｔｏｒｙｆａｃｔｏｒｓｄｕｒｉｎｇｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｍａｍｍａｌｉａｎｅｒｙｔｈｒｏｃｙｔｅ,ａｃｔｉｎｇｍａｉｎｌｙｏｎｔｈｅｅｒｙｔｈｒ...     

水泥黄土增强措施初探

在分析资料和室内试验的基础上,概括了提高水泥黄土强度有效措施,就水泥黄土硬化和外加剂对水泥黄土的作用机理进行了初步探讨。