高压静电场处理油菜种子的品质影响及模型探讨

研究了高压静电场处理种子在大田生长条件下的长期效应 .结果表明 ,较低电压的处理可提高硫甙含量显著降低含油量 ,而较高电压的处理可降低硫甙含量 ,极显著降低芥酸含量 .采用平面类脂双层模型分析外加高压静电场对植物细胞跨膜电位的影响 ,并探讨其微观机理 ,一定程度上可以解释相关的实验结果     

Relationship between the exposure dose of high intensity focused ultrasound and the heated necrosis element

Heated necrosis element of tissues is the basis for treating tumor by high intensity focused ultrasound (HIFU), so study on the relationship between the HIFU exposure dose and the heated necrosis element of tissues is of great significance for both HIFU clinical applications and basic research. Using Pennes and Rayleigh formulae the relationship between the exposure dose of an annular focused transducer and the heated necrosis element (I.e. Biological focused region) in a bovine liver in vitro at a depth of 2 cm is simulated in an exposure dose range with radiation sound intensity of 7000～25400 W/cm2 and exposure time of 0～20 s. The theoretical results appear to be in quite good agreement with the experimental ones. It is shown that on the basis of existing theories the heated necrosis element produced by HIFU exposure can be predicted. The deviation of the theory from the experiments under higher exposure dose has also been discussed.     

Difference schemes on non-uniform mesh and their application

High order accurate schemes are needed to simulate the multi-scale complex flow fields to get fine structures in simulation of the complex flows with large gradient of fluid parameters near the wall, and schemes on non-uniform mesh are desirable for many CFD (computational fluid dynamics) workers. The construction methods of difference approximations and several difference approximations on non-uniform mesh are presented. The accuracy of the methods and the influence of stretch ratio of the neighbor mesh increment on accuracy are discussed. Some comments on these methods are given, and comparison of the accuracy of the results obtained by schemes based on both non-uniform mesh and coordinate transformation is made, and some numerical examples with non-uniform mesh are presented.     

高科技企业的价值链管理

分析了波特基本价值链在应用上的局限性 ,结合高科技企业的特点对波特价值链的构成要素和要素排列方式进行改进 ,构造了高科技企业的价值链模型 ,并从三个方面阐述了高科技企业价值链管理区别于传统企业的主要特征     

重组人尿激酶原氨基末端片段的制备及活性测定

尿激酶原的N-末端135个氨基酸片段包含了表皮生长因子结构域和环柄结构域，参与了尿激酶原和其受体的结合以及尿激酶原诱导的化学趋化活性．利用RT-PCR，从人脐带静脉内皮细胞中克隆ATF基因．将ATF基因插入pET-29a( )中构建表达质粒pET-29a( )／ATF，并转化至大肠杆菌BL21(DE3)中，经IPTG诱导表达及纯化后，从每升培养液中获得15mg rhATF，其纯度超过95％．活性测定结果表明rhATF能够阻断尿激酶原与尿激酶受体的结合，并能抑制尿激酶对纤溶酶原以及纤溶酶对尿激酶原的激活．     

基于编队卫星的SAR高方位向分辨率成像

针对编队卫星被动工作方式下提高SAR方位向分辨率的构型,在建立信号回波模型和详细分析提高方位向分辨率原理的基础上,提出了对基线的要求及频谱合成算法。该算法充分利用了编队卫星SAR系统中不同接收机的视角信息,有效地完成了多组不同多普勒位置的信号相干合成,实现了系统分辨率的提高。计算机仿真结果表明该算法具有良好的性能。     

耐高温耐高酒度醋酸菌选育研究

从高温季节发酵良好的醋醅中,分离筛选出N 9、F17两株醋酸菌,在培养温度35℃,培养基酒度6%(V/V)条件下,产酸量均高于A S1.41号.以N 9、F17为出发菌株,经紫外线、硫酸二乙酯逐级诱变,分别获得编号为HN 15、HF6两株耐高温耐高酒度醋酸菌株.在培养温度40℃,培养基酒度12%(V/V)条件下,HN 15酒精转化率为75%,HF6酒精转化率为70%.     

HLA RTI数据描述码扩展

为高层体系结构(High Level Architecture，HLA)运行支撑环境(Run—Time Infrastructure，RTI)引入一个新的扩展，该扩展使用数据描述码在消息上附加特征属性，使RTI能够对数据应用更丰富的使用策略。文章第1、2部分提出了基于数据描述码过滤数据，以及构建HLA仿真系统面向方面框架(Aspect Oriented Framework，AOF)的技术，第3、4部分详细说明了数据描述码扩展和实验原型的设计。数据描述码扩展可用于提高仿真系统的运行效率，改进大规模HLA仿真系统的架构设计，并且不改变RTI和联邦成员的编程接口。     

高效液相色谱法快速检测海藻糖

旨在建立一种利用高效液相色谱法检测海藻糖的方法.实验采用Brevibacterium helvolum菌转化液化淀粉生产海藻糖.在反应混合物中,麦芽糖对海藻糖的检测有显著影响.通过改变乙腈和水的比例,麦芽糖和海藻糖最终被完全分开.高效液相色谱条件为：碳水化合物分析柱;洗脱剂为V（乙腈）：V（水）＝80：20;体积流量1 mL/min;进样量20 μL;柱温为室温.麦芽糖和海藻糖的保留时间分别为11.3 min和13.8 min.